Abstract:
:In this study, the effects of the accumulation of cysteine protease inhibitors on the food preferences of adult female western flower thrips, Frankliniella occidentalis (Pergande), were investigated. Representative members of the cystatin and thyropin gene families (stefin A, cystatin C, kininogen domain 3 and equistatin) were expressed in potato (Solanum tuberosum) cv. Impala, Kondor and Line V plants. In choice assays, a strong time- and concentration-dependent deterrence from plants expressing stefin A and equistatin was observed. Cystatin C and kininogen domain 3 were not found to be active. All tested inhibitors were equally or more active than stefin A at inhibiting the proteolytic activity of thrips, but, in contrast with stefin A, they were all expressed in potato as partially degraded proteins. The resistance of cysteine protease inhibitors against degradation in planta by endogenous plant proteases may therefore be relevant in explaining the observed differences in the deterrence of thrips. The results demonstrate that, when given a choice, western flower thrips will select plants with low levels of certain cysteine protease inhibitors. The novel implications of the defensive role of plant cysteine protease inhibitors as both deterrents and antimetabolic proteins are discussed.
journal_name
Plant Biotechnol Jjournal_title
Plant biotechnology journalauthors
Outchkourov NS,de Kogel WJ,Schuurman-de Bruin A,Abrahamson M,Jongsma MAdoi
10.1111/j.1467-7652.2004.00088.xsubject
Has Abstractpub_date
2004-09-01 00:00:00pages
439-48issue
5eissn
1467-7644issn
1467-7652pii
PBI088journal_volume
2pub_type
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