BioNano genome mapping of individual chromosomes supports physical mapping and sequence assembly in complex plant genomes.

abstract：:We describe the site-directed integration (SDI) system for Agrobacterium-mediated transformation to precisely integrate a single copy of a desired gene into a predefined target locus by recombinase-mediated cassette exchange (RMCE). The system requires the selection of a transformed line with an integrated copy of a t...

journal_title：Plant biotechnology journal

authors： Nanto K,Yamada-Watanabe K,Ebinuma H

更新日期：2005-03-01 00:00:00

abstract：:There is a pressing need to develop novel antibacterial agents given the widespread antibiotic resistance among pathogenic bacteria and the low specificity of the drugs available. Endolysins are antibacterial proteins that are produced by bacteriophage-infected cells to digest the bacterial cell wall for phage progeny...

journal_title：Plant biotechnology journal

authors： Stoffels L,Taunt HN,Charalambous B,Purton S

更新日期：2017-09-01 00:00:00

abstract：:Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

journal_title：Plant biotechnology journal

authors： Wu CS,Chaw SM

更新日期：2014-04-01 00:00:00

abstract：:Flavonoids are widely distributed in plants and play important roles in human and animal health and nutrition. Model plants with discernible flavonoid phenotypes, such as Arabidopsis seed patterning lines, are valuable tools that can provide avenues for understanding flavonoid and proanthocyanidin accumulation pattern...

journal_title：Plant biotechnology journal

authors： Gao P,Li X,Cui D,Wu L,Parkin I,Gruber MY

更新日期：2010-12-01 00:00:00

abstract：:Downy mildew (Plasmopara halstedii (Farl.) Berlese et de Toni) is a serious foliar pathogen of cultivated sunflower (Helianthus annuus L.). Genetic resistance is conditioned by several linked downy mildew resistance gene specificities in the HaRGC1 cluster of TIR-NBS-LRR resistance gene candidates (RGCs) on linkage gr...

journal_title：Plant biotechnology journal

authors： Slabaugh MB,Yu JK,Tang S,Heesacker A,Hu X,Lu G,Bidney D,Han F,Knapp SJ

更新日期：2003-05-01 00:00:00

abstract：:Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....

journal_title：Plant biotechnology journal

authors： Furtado A,Henry RJ,Pellegrineschi A

更新日期：2009-04-01 00:00:00

abstract：:Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...

journal_title：Plant biotechnology journal

authors： Gutierrez L,Mauriat M,Guénin S,Pelloux J,Lefebvre JF,Louvet R,Rusterucci C,Moritz T,Guerineau F,Bellini C,Van Wuytswinkel O

更新日期：2008-08-01 00:00:00

abstract：:The tomato PROCERA gene encodes a DELLA protein, and loss-of-function mutations derepress growth. We used CRISPR/Cas9 and a single guide RNAs (sgRNA) to target mutations to the PROCERA DELLA domain, and recovered several loss-of-function mutations and a dominant dwarf mutation that carries a deletion of one amino acid...

journal_title：Plant biotechnology journal

authors： Tomlinson L,Yang Y,Emenecker R,Smoker M,Taylor J,Perkins S,Smith J,MacLean D,Olszewski NE,Jones JDG

更新日期：2019-01-01 00:00:00

abstract：:Pollen germination and pollen tube growth are important physiological processes of sexual reproduction of plants and also are involved in signal transduction. Our previous study reveals that ZmSTK1 and ZmSTK2 are two receptor-like cytoplasmic kinases (RLCK) homologs in Zea mays as members of receptor-like protein kina...

journal_title：Plant biotechnology journal

authors： Fan M,Zhang C,Shi L,Liu C,Ma W,Chen M,Liu K,Cai F,Wang G,Wei Z,Jiang M,Liu Z,Javeed A,Lin F

更新日期：2018-08-01 00:00:00

abstract：:Recently, CRISPR-Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed CRISPR-LbCas12a (LbCpf1), which is derived from Lachnospiraceae bacterium ND2006, to edit a citrus genome for the first time. First, LbCas12a was used to modify the CsPDS gene successfully...

journal_title：Plant biotechnology journal

authors： Jia H,Orbović V,Wang N

更新日期：2019-10-01 00:00:00

abstract：:Squalene is a linear intermediate to nearly all classes of triterpenes and sterols and is itself highly valued for its use in wide range of industrial applications. Another unique linear triterpene is botryococcene and its methylated derivatives generated by the alga Botryococcus braunii race B, which are progenitors ...

journal_title：Plant biotechnology journal

authors： Jiang Z,Kempinski C,Kumar S,Kinison S,Linscott K,Nybo E,Janze S,Wood C,Chappell J

更新日期：2018-06-01 00:00:00

abstract：:Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistanc...

journal_title：Plant biotechnology journal

authors： Peng A,Chen S,Lei T,Xu L,He Y,Wu L,Yao L,Zou X

更新日期：2017-12-01 00:00:00

abstract：:Although plant biotechnology has been widely investigated for the production of clinical-grade monoclonal antibodies, no antibody products derived from transgenic plants have yet been approved by pharmaceutical regulators for clinical testing. In the Pharma-Planta project, the HIV-neutralizing human monoclonal antibod...

journal_title：Plant biotechnology journal

authors： Ma JK,Drossard J,Lewis D,Altmann F,Boyle J,Christou P,Cole T,Dale P,van Dolleweerd CJ,Isitt V,Katinger D,Lobedan M,Mertens H,Paul MJ,Rademacher T,Sack M,Hundleby PA,Stiegler G,Stoger E,Twyman RM,Vcelar B,Fischer

更新日期：2015-10-01 00:00:00

abstract：:Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

journal_title：Plant biotechnology journal

authors： Czerednik A,Busscher M,Angenent GC,de Maagd RA

更新日期：2015-02-01 00:00:00

abstract：:Sugarcane (Saccharum spp.) is a highly energy-efficient crop primarily for sugar and bio-ethanol production. Sugarcane genetics and cultivar improvement have been extremely challenging largely due to its complex genomes with high polyploidy levels. In this study, we deeply sequenced the coding regions of 307 sugarcane...

journal_title：Plant biotechnology journal

authors： Yang X,Song J,Todd J,Peng Z,Paudel D,Luo Z,Ma X,You Q,Hanson E,Zhao Z,Zhao Y,Zhang J,Ming R,Wang J

更新日期：2019-02-01 00:00:00

abstract：:The capacity of the bread wheat (Triticum aestivum) genome to tolerate introgression from related genomes can be exploited for wheat improvement. A resistance to powdery mildew expressed by a derivative of the cross-bread wheat cv. Tähti × T. militinae (Tm) is known to be due to the incorporation of a Tm segment into ...

journal_title：Plant biotechnology journal

authors： Abrouk M,Balcárková B,Šimková H,Komínkova E,Martis MM,Jakobson I,Timofejeva L,Rey E,Vrána J,Kilian A,Järve K,Doležel J,Valárik M

更新日期：2017-02-01 00:00:00

abstract：:To date, transgenic approaches to biofortify subsistence crops have been rather limited. This is particularly true for the starchy root crop cassava (Manihot esculenta Crantz). Cassava has one of the highest rates of CO(2) fixation and sucrose synthesis for any C3 plant, but rarely reaches its yield potentials in the ...

journal_title：Plant biotechnology journal

authors： Ihemere U,Arias-Garzon D,Lawrence S,Sayre R

更新日期：2006-07-01 00:00:00

abstract：:Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...

journal_title：Plant biotechnology journal

authors： Gámez-Arjona FM,Li J,Raynaud S,Baroja-Fernández E,Muñoz FJ,Ovecka M,Ragel P,Bahaji A,Pozueta-Romero J,Mérida Á

更新日期：2011-12-01 00:00:00

abstract：:Tomato (Solanum lycopersicum) fruit ripening is regulated co-operatively by the action of ethylene and a hierarchy of transcription factors, including RIPENING INHIBITOR (RIN) and NON-RIPENING (NOR). Mutations in these two genes have been adopted commercially to delay ripening, and accompanying textural deterioration,...

journal_title：Plant biotechnology journal

authors： Osorio S,Carneiro RT,Lytovchenko A,McQuinn R,Sørensen I,Vallarino JG,Giovannoni JJ,Fernie AR,Rose JKC

更新日期：2020-01-01 00:00:00

abstract：:Maize kernels do not contain enough of the essential sulphur-amino acid methionine (Met) to serve as a complete diet for animals, even though maize has the genetic capacity to store Met in kernels. Prior studies indicated that the availability of the sulphur (S)-amino acids may limit their incorporation into seed stor...

journal_title：Plant biotechnology journal

authors： Xiang X,Wu Y,Planta J,Messing J,Leustek T

更新日期：2018-05-01 00:00:00

abstract：:Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

journal_title：Plant biotechnology journal

authors： Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

更新日期：2013-02-01 00:00:00

abstract：:Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

journal_title：Plant biotechnology journal

authors： Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

更新日期：2014-05-01 00:00:00

abstract：:Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-Arg dipeptide...

journal_title：Plant biotechnology journal

authors： Ponndorf D,Ehmke S,Walliser B,Thoss K,Unger C,Görs S,Daş G,Metges CC,Broer I,Nausch H

更新日期：2017-05-01 00:00:00

abstract：:Modified fatty acids (mFA) have diverse uses; for example, cyclopropane fatty acids (CPA) are feedstocks for producing coatings, lubricants, plastics and cosmetics. The expression of mFA-producing enzymes in crop and model plants generally results in lower levels of mFA accumulation than in their natural-occurring sou...

journal_title：Plant biotechnology journal

authors： Yu XH,Cahoon RE,Horn PJ,Shi H,Prakash RR,Cai Y,Hearney M,Chapman KD,Cahoon EB,Schwender J,Shanklin J

更新日期：2018-04-01 00:00:00

abstract：:The importance and urgency of providing humans and animals with quality proteins are reflected in the growing scientific and industrial interest in augmenting the nutritive value of the world's protein sources. Such nutritive value is determined by the protein content in 'essential amino acids', those that cannot be s...

journal_title：Plant biotechnology journal

authors： Beauregard M,Hefford MA

更新日期：2006-09-01 00:00:00

abstract：:Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...

journal_title：Plant biotechnology journal

authors： Redillas MC,Jeong JS,Kim YS,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2012-09-01 00:00:00

abstract：:Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system ...

journal_title：Plant biotechnology journal

authors： Zhang J,Yin K,Sun J,Gao J,Du Q,Li H,Qiu JL

更新日期：2018-02-01 00:00:00

abstract：:Expression of industrial enzymes in transgenic plants offers an alternative system to fungal fermentation for large-scale production. Very high levels of expression are required to make the enzymes cost-effective. We tested several parameters to determine the best method for achieving high levels of expression for a f...

journal_title：Plant biotechnology journal

authors： Hood EE,Bailey MR,Beifuss K,Magallanes-Lundback M,Horn ME,Callaway E,Drees C,Delaney DE,Clough R,Howard JA

更新日期：2003-03-01 00:00:00

abstract：:Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known...

journal_title：Plant biotechnology journal

authors： Kumar V,Chattopadhyay A,Ghosh S,Irfan M,Chakraborty N,Chakraborty S,Datta A

更新日期：2016-06-01 00:00:00

abstract：:Grain size and plant architecture are critical factors determining crop productivity. Here, we performed gene editing of the MIR396 gene family in rice and found that MIR396e and MIR396f are two important regulators of grain size and plant architecture. mir396ef mutations can increase grain yield by increasing grain s...

journal_title：Plant biotechnology journal

authors： Miao C,Wang D,He R,Liu S,Zhu JK

更新日期：2020-02-01 00:00:00