解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Although the amino acid sequences and the structures of pyruvate kinase (PYK) isozymes are highly conserved, allosteric regulations differ. This suggests that amino acids with low conservation play important roles in the allosteric mechanism. The current work exploits a 'natural screen'- the 122 point mutations identi...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500531705
更新日期:2006-01-01 00:00:00
abstract::Accumulating evidence indicates that there is a critical role of the ubiquitin/proteasome pathway in the regulation of apoptosis. Among the important molecules that couple these two fundamental cellular activities are members of the inhibitor of apoptosis (IAP) protein family. In addition to their well-studied ability...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500389013
更新日期:2005-12-01 00:00:00
abstract::The extensive post-transcriptional modification of U2 snRNA is required for spliceosome assembly and pre-mRNA splicing in vertebrates. However, the rare modification of U2 snRNA in yeast implies a different mechanism for regulating spliceosome biogenesis in single-celled eukaryotes. To understand the evolutionary patt...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500306983
更新日期:2005-10-01 00:00:00
abstract::Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15 N-labeled proteins from 15 N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500217859
更新日期:2005-09-01 00:00:00
abstract::Transketolase (TK) is a homodimer, the simplest representative of thiamine diphosphate (ThDP)-dependent enzymes. It was first ThDP-dependent enzymes the crystal structure of which has been solved and revealed the general fold for this class of enzymes and the interactions of the non-covalently bound coenzyme ThDP with...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500167203
更新日期:2005-07-01 00:00:00
abstract::The last 15 years of effort in understanding bacterial DNA replication and repair has identified that the donut shaped beta2 sliding clamp is harnessed by very functionally different DNA polymerases throughout the lifecycle of the bacterial cell. Remarkably, the sites of binding of these polymerases, in most cases, ap...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500138246
更新日期:2005-06-01 00:00:00
abstract::Bovine Spongiform Encephalopathy (BSE) is typical of the dementias that affect both animals and man; Scrapie in sheep, Creutzfeldt-Jakob disease in man. Global efforts have been made to determine the nature of the active agents in these diseases. At present the 'protein only hypothesis' of Prusiner holds. It was a sur...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500097152
更新日期:2005-04-01 00:00:00
abstract::Recently the crystal structure of creatine kinase from Torpedocalifornica was determined to 2.1 A. The dimeric structure revealed two different forms in the unit cell: one monomer was bound to a substrate, MgADP, and the other monomer was bound to a transition-state analogue complex composed of MgADP, nitrate and crea...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500091999
更新日期:2005-04-01 00:00:00
abstract::Hydrogen peroxide is a major regulator of plant programmed cell death (PCD) but little is known about the downstream genes from the H(2)O(2)-signaling network that mediate the cell death. To address this question, a novel system for studying H(2)O(2)-induced programmed cell death in Arabidopsis thaliana was used. The ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500090793
更新日期:2005-03-01 00:00:00
abstract::The rat organic anion transporter 3 (rOAT3) has recently been identified as the third isoform of the OAT family. The mechanisms that regulate rOAT3's functions remain to be elucidated. rOAT3 contributes for moving a number of negatively charged organic compounds between cells and their extracellular milieu. Caveolin (...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540500104750
更新日期:2005-02-01 00:00:00
abstract::Transforming growth factorbeta1 (TGFbeta1) is considered to be the principal contributor to liver fibrosis. So in this study the ribozymes against TGFbeta1 were designed. The in vitro cleavage activities of the ribozymes were assayed through incubation of (32)p-labeled target RNAs and (32)p-labeled ribozymes in differ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400024470
更新日期:2005-01-01 00:00:00
abstract::The physiological role of neuroglobin and cytoglobin, two vertebrate globins discovered in the last 5 years, is not yet clearly understood. In this work, we review the structural information on these globins and its implication on the possible protein function, obtained by electron paramagnetic resonance and resonance...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540500037877
更新日期:2004-11-01 00:00:00
abstract::Large inter-individual variation exists in the response to vitamin E supplementation, and this may influence the outcome of human studies. It is our hypothesis that genetic heterogeneity is an important determinant of vitamin E homeostasis. Therefore we have performed an in silico search for single nucleotide polymorp...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400020346
更新日期:2004-10-01 00:00:00
abstract::Cyclic AMP metabolism is essential for the survival of the social amoebae Dictyostelium discoideum. Three distinct adenylyl cyclases are expressed and required for the normal development of this simple eukaryote. The adenylyl cyclase expressed during aggregation, ACA, is related to the mammalian and Drosophila G prote...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540400013887
更新日期:2004-09-01 00:00:00
abstract::The activation and incorporation of selenium into selenocysteine containing selenoproteins has been well established in an Escherichia coli model system but there is little specific information concerning the transport and intracellular trafficking of selenium in biological systems in general. A selenium transport rol...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400010818
更新日期:2004-08-01 00:00:00
abstract::Selenium is an essential component of several enzymes and proteins in a number of methane-producing archae. Information concerning accessory proteins that function in selenium transport processes, however, is limited. A novel selenium-binding protein with a potential transport role and a selenocysteine lyase that serv...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540400008911
更新日期:2004-07-01 00:00:00
abstract::Availability of the human genome data has enabled the exploration of a huge amount of biological information encoded in it. There are extensive ongoing experimental efforts to understand the biological functions of the gene products encoded in the human genome. However, computational analysis can aid immensely in the ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540400006105
更新日期:2004-06-01 00:00:00
abstract::Treatment of microsomes (preferably enriched with endoplasmic reticulum) isolated from bovine pulmonary artery smooth muscle tissue with the O2*- -generating system (hypoxanthine (HPX) plus xanthine oxidase (XO)), markedly stimulated matrix metalloproteinase-2 (MMP-2) activity and also enhanced Ca2+ ATPase activity an...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654041000171335
更新日期:2004-05-01 00:00:00
abstract::The purpose of this study was to confirm protein-protein interaction between cyclooxygenase-1 (COX-1) and caveolins. The interaction of cyclooxygenase-1 and caveolins in the cultured human embryonic kidney (HEK 293) cells was investigated using immuno-precipitation and Western blot analysis. In HEK 293 cells, high lev...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540410001699312
更新日期:2004-04-01 00:00:00
abstract::Mutations in the breast cancer susceptibility gene BRCA1 predispose individuals to breast and ovarian cancers. Cofactor of BRCA1 (COBRA1), a novel protein, was isolated as a BRCA1-interacting protein. However, the role of COBRA1 in breast cancer is poorly understood. In this study, we demonstrate that COBRA1 mRNA was ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540410001699826
更新日期:2004-03-01 00:00:00
abstract::Cancers have a clonal origin, yet their chromosomes and genes are non-clonal or heterogeneous due to an inherent genomic instability. However, the cause of this genomic instability is still debated. One theory postulates that mutations in genes that are involved in DNA repair and in chromosome segregation are the prim...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540410001667902
更新日期:2004-02-01 00:00:00
abstract::How should a protein be designed to serve in processes as diverse as chromosome condensation, sister chromatid cohesion, DNA recombination, gene dosage regulation, and perhaps even gene silencing or transcriptional regulation--which occur in both mitosis and meiosis? Such a protein or protein complex needs to bear DNA...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540310001639661
更新日期:2003-12-01 00:00:00
abstract::Peroxynitrite is an inorganic toxin of physiological interest, formed from the diffusion-controlled reaction of superoxide and nitrogen monoxide with a rate constant of (1.6 +/- 0.3) x 10(10) M(-1) s(-1). On the basis of three experiments we conclude that homolysis of the O-O bond in peroxynitrous acid is unlikely: (1...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540310001628690
更新日期:2003-10-01 00:00:00
abstract::Reactive oxygen and nitrogen species (ROS and RNS) have been extensively recognized as important signaling molecules implicated in physiological processes such as gene expression, cell differentiation and immune activation. Nevertheless, continuous production of these species may produce oxidative and/or nitrosative s...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540310001628717
更新日期:2003-10-01 00:00:00
abstract::From a liver cDNA library prepared from a nonvenomous striated snake, Elaphe quadrivirgata, we isolated a cDNA encoding a novel protein, PLIalpha-like protein (PLIalpha-LP), having approximately 70% sequence identities with the alpha-type phospholipase A2 (PLA2) inhibitors (PLIalpha(s)) previously purified from the ve...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540310001620995
更新日期:2003-09-01 00:00:00
abstract::In many organisms the processes of DNA replication and recombination are closely linked. For instance, in bacterial and eukaryotic systems, replication forks can become stalled or damaged, in many cases leading to the formation of double stranded breaks. Replication restart is an essential mechanism in which the recom...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540310001612237
更新日期:2003-08-01 00:00:00
abstract::Cocaine is a powerful central nervous stimulant and among the most abused of drugs. Despite decades of efforts, however, no effective pharmacological treatments are available against cocaine addiction or toxic effects. Classical receptor-antagonist therapeutic approaches have not yielded significant effects, although ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/1521654031000138541
更新日期:2003-07-01 00:00:00
abstract::Recent successes in identifying the genes and associated proteins underlying several familial neurodegenerative conditions have not always resulted in accounts as to why the associated patterns of neuronal damage are so specific and limited. Here, with reference to Huntington's disease, we present a general scheme to ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/1521654031000153012
更新日期:2003-06-01 00:00:00
abstract::Mitochondria, the energy-producing organelles of the eukaryotic cell, originate from an endosymbiotic alpha-proteobacterium. These organelles are believed to have arisen only once in evolutionary history, but despite their common ancestry, mitochondrial DNAs vary extensively throughout eukaryotes in genome architectur...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/1521654031000137380
更新日期:2003-04-01 00:00:00
abstract::We have adapted the Java Molecular Viewer (JMV) to virtual reality display environments, through a number of extensions to the Java 3D code. Phylogenetic information derived from multiple alignments (temporal information) can be overlaid onto molecule structures (spatial information). The number of sequences included ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654031000136255
更新日期:2003-04-01 00:00:00
abstract::The pathogenetic mechanism of the most extensively investigated A3243G mutated tRNALeu(UUR) gene, which causes the MELAS encephalomyopathy, maternally inherited diabetes, or chronic progressive external ophlthalmoplegia, is still unresolved, despite the numerous investigations on the topic. Previous evidences presente...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654031000110190
更新日期:2003-03-01 00:00:00
abstract::The yeast Saccharomyces cerevisiae is an excellent model for gaining insights into the molecular basis of human mitochondrial disorders, particularly those resulting from impaired mitochondrial metabolism. Yeast is a very well characterized system and most of our current knowledge about mitochondrial biogenesis in hum...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1002/tbmb.718540876
更新日期:2003-02-01 00:00:00
abstract::The human nuclear estrogen receptors (i.e., ERalpha and ERbeta), as other transcriptional factors, regulate cellular processes inducing genomic events. In addition, the binding of 17beta-estradiol to ERs induces different membrane initiating non-genomic signaling. The non-genomic effects of ER are independent of trans...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/1521654031000081256
更新日期:2003-01-01 00:00:00
abstract::We investigated signaling pathways leading to tumor necrosis factor (TNF) alpha-induced intercellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression in chondrosarcoma cells, and determined the functional significance of their expression by examining Jurkat T cell adhesion. TNFalpha ...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540215674
更新日期:2002-11-01 00:00:00
abstract::The beta/alpha-barrel motif was once considered to be a single protein domain. In recent years, however, it has been shown to consist of smaller substructures displaying the ability to fold autonomously. Here we review the current status of experimental findings concerning the motif's folding behavior in the light of ...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540214924
更新日期:2002-10-01 00:00:00
abstract::Role of nitric oxide (NO) on the expression of organic anion transporter 2 (OAT2) located in sinusoidal domain of hepatocytes has been investigated. Effect of NO generated in vivo in rat and delivered in vitro to hepatocytes was determined. Lipopolysaccharide (LPS) and spermine NONOate (SPER/NO) were selected as the N...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540214534
更新日期:2002-09-01 00:00:00
abstract::Heart disease is the most common cause of morbidity and mortality in Western society and the incidence is projected to increase significantly over the next few decades as our population ages. Heart failure occurs when the heart is unable to pump blood at a rate to commensurate with tissue metabolic requirements and re...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540214312
更新日期:2002-08-01 00:00:00
abstract::Xanthomonas campestris pv. campestris, a plant-associated pathogenic bacterium, is the causal agent of foliar spots and blights in crucifers. The major outer membrane protein, Omp37, of 37 kDa, has been identified, purified to homogeneity, and its characterization has also been carried out. Native Omp37 behaved as a t...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540213826
更新日期:2002-07-01 00:00:00
abstract::The functional effect of the interaction of E2F1 and hepatitis B virus X protein (HBx) on the promoter of human p53 gene was studied using chloramphenicol acetyl transferase (CAT) assay. E2F1 activated the p53 promoter through E2F1 binding site. As previously reported, HBx repressed the p53 promoter through E-box. Whe...
journal_title:IUBMB life
pub_type: 杂志文章
doi:10.1080/15216540213466
更新日期:2002-06-01 00:00:00
abstract::The existence of a genetic component in mycobacterial disease susceptibility is no longer in doubt and the investigations now being conducted aim to determine which genes are involved, to what extent, and in which disease phenotype they are relevant. In certain rare instances of susceptibility to poorly pathogenic myc...
journal_title:IUBMB life
pub_type: 杂志文章,评审
doi:10.1080/15216540212644
更新日期:2002-04-01 00:00:00