Abstract:
:The last 15 years of effort in understanding bacterial DNA replication and repair has identified that the donut shaped beta2 sliding clamp is harnessed by very functionally different DNA polymerases throughout the lifecycle of the bacterial cell. Remarkably, the sites of binding of these polymerases, in most cases, appear to be the same shallow pocket on the beta dimer. In every case, binding of beta2 by the polymerase enhances their processivity of DNA synthesis. This binding site is also the same point of interaction between beta2 and the clamp loader complex, which binds beta2, opens and places it onto the DNA strand and then vacates the site. Beta2 may also be involved in the initiation of DNA replication again via contact through this same site. While much of the research effort has focused on Escherichia coli and Bacillus subtilis, conservation of this complex system is becoming apparent in diverse bacteria.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Wijffels G,Dalrymple B,Kongsuwan K,Dixon NEdoi
10.1080/15216540500138246keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
413-9issue
6eissn
1521-6543issn
1521-6551pii
U4056710213N2107journal_volume
57pub_type
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