Abstract:
:Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15 N-labeled proteins from 15 N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concentration or chromatographic purification of the protein. We review methodological aspects of cell-free in vitro protein synthesis based on an Escherichia coli cell extract, in particular with regard to the production of 15 N-labeled proteins for analysis by NMR spectroscopy.
journal_name
IUBMB Lifejournal_title
IUBMB lifeauthors
Ozawa K,Dixon NE,Otting Gdoi
10.1080/15216540500217859keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
615-22issue
9eissn
1521-6543issn
1521-6551pii
X3V1751203806263journal_volume
57pub_type
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