解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract:BACKGROUND:Cardiovascular mortality is markedly higher in Russia than in Western Europe and the US. Little is known about indicators of atherosclerotic risk in the Russian population. To our knowledge, this is the first study of apolipoprotein (apo) AI and B in Russia based on the WHO-IFCC standard. METHODS:We measure...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.023853
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:Our previously developed assay for detection of the factor V Leiden mutation (G1691A) based on a nucleic acid photo-cross-linking technology used two allele-specific capture probes and six fluorescein-modified signal-generating reporter probes. We wished to improve the sensitivity and performance of the meth...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.023556
更新日期:2004-02-01 00:00:00
abstract:BACKGROUND:Determinations of certain forms of prostate-specific antigen (PSA) have been shown to increase the specificity for prostate cancer (PCa). One such variant, proteolytically active PSA, is a potentially useful tumor marker, but it is not specifically recognized by antibodies. Using phage display libraries, we ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.026146
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.016311
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:Test repetition could be a readily modifiable component of laboratory utilization. Laboratory test repetition has not been rigorously studied at a population-based level. Our objective was to determine the prevalence of, and charges associated with, repetition of eight common laboratory tests. METHODS:We pe...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.021220
更新日期:2003-12-01 00:00:00
abstract::It has been estimated that approximately 37% of the US population judged to be at high risk for developing coronary artery disease (CAD), based on the National Cholesterol Education Program guidelines, have increased plasma lipoprotein(a) [Lp(a)], whereas Lp(a) is increased in only 14% of those judged to be at low ris...
journal_title:Clinical chemistry
pub_type:
doi:10.1373/clinchem.2003.023689
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.023341
更新日期:2003-11-01 00:00:00
abstract:BACKGROUND:Laboratory tests can be an important source of blood loss in hospitals, especially for newborns and patients in intensive care. The aim of this study was to quantify blood loss for laboratory diagnostic tests in a large number of patients in a teaching hospital. METHODS:We estimated blood loss by multiplyin...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.10.1651
更新日期:2003-10-01 00:00:00
abstract:BACKGROUND:Paraoxonase may protect lipoproteins and cell membranes from peroxidation, and alterations in the activity of this enzyme have been associated with some chronic diseases. Serum paraoxonase appears to be mainly under genetic control, but some studies suggest that environmental factors may also modulate its ac...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.9.1491
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:C-reactive protein (CRP) is a widely recognized indicator of inflammation and is known to play an important role in atherogenesis. Recent prospective studies have demonstrated that increased CRP concentrations within the reference interval are a strong predictor of myocardial infarction, stroke, sudden cardi...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/49.8.1258
更新日期:2003-08-01 00:00:00
abstract:BACKGROUND:The aims of this study were to measure the N-terminal fragment of pro-brain natriuretic peptide (proBNP) in plasma in medical conditions commonly found in primary care and to evaluate the utility of these measurements in identifying impaired cardiac function in elderly patients with symptoms associated with ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.8.1337
更新日期:2003-08-01 00:00:00
abstract:BACKGROUND:Two mutations in HFE, G845A (amino acid substitution C282Y) and C187G (H63D), are associated with hereditary hemochromatosis. We developed and validated a novel method, linked linear amplification (LLA), for detection of these two mutations. METHODS:Two segments of HFE were amplified by a multiplex LLA reac...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.7.1050
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:D-2-Hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.7.1133
更新日期:2003-07-01 00:00:00
abstract:BACKGROUND:Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive a...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.6.861
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.6.935
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:External quality assurance (EQA) programs for diagnostic tests based on nucleic acid amplification have not been widely implemented in clinical laboratories and remain limited to few tests. Development of specific EQA programs based on application-based proficiency testing for any diagnostic molecular target...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.5.782
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:Clinical diagnoses were correlated with results of a Co(II)-albumin binding assay in 167 patients treated at an emergency department of a health maintenance organization. METHODS:Patients were evaluated as being nonischemic or potentially ischemic through standard coronary disease indicators [creatine kinas...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:10.1373/49.4.581
更新日期:2003-04-01 00:00:00
abstract:BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.3.389
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Uremic syndrome is the consequence of the retention of solutes usually cleared by the healthy kidneys. p-Cresol can be considered a prototypic protein-bound uremic toxin. It is conceivable, analogous with drugs, that the non-protein-bound fraction of p-cresol exerts toxicity. This aspect had never been evalu...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.3.470
更新日期:2003-03-01 00:00:00
abstract:BACKGROUND:Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS:Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.2.269
更新日期:2003-02-01 00:00:00
abstract:BACKGROUND:An abnormal IgLkappa:IgLlambda ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLkappa:IgLlambda ratio in clinical sample...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.1.51
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Some of the genetic variants of hemoglobin (Hb) and their chemically modified species are known to affect the measurement of Hb A(1c). The purpose of this study was to characterize Hb species in the blood sample of a 74-year-old German male with an exceptionally low Hb A(1c) value. METHODS:Hemolysates from ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.1.137
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS:After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleo...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:Because cyclosporin A (CsA) and glucocorticoids inhibit the production of interleukin-2 (IL-2) and other cytokines, quantitative analysis of cytokine mRNA might constitute a pharmacodynamic measure for immunosuppressive drug effects. We investigated whether immunosuppressive drugs influence cytokine mRNA exp...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:The analytical and clinical performance of homogeneous LDL-cholesterol assays has been reported, but their reactions with subfractions of LDL and VLDL have not been described in detail. METHODS:We evaluated reaction selectivity of two homogeneous LDL-cholesterol assays, LDLk (Kyowa Medex) and LDLd (Daiichi ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:The management of critically ill infants and neonates includes frequent determination of arterial blood gas, electrolyte, and hematocrit values. An objective of attached point-of-care patient monitoring is to provide clinically relevant data without the adverse consequences associated with serial phlebotomy....
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:
更新日期:2002-11-01 00:00:00
abstract:BACKGROUND:To allow multianalyte binding assays, we have developed a novel polystyrene microtiter plate containing 24 main wells, each divided into 7 subwells. We explored its clinical potential by developing a PCR-chemiluminescent immunoassay (PCR-CLEIA) for simultaneous detection and typing of seven high oncogenic ri...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Rapid intraoperative parathyroid hormone (PTH) measurements are an important prerequisite for minimally invasive parathyroidectomy, serving as a feasible marker for "cure" because of the short half-life of PTH. Because automated analysis may facilitate monitoring, two automated PTH assays were compared with ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:The detection of monoclonal free light chains (FLCs) is an important diagnostic aid for a variety of monoclonal gammopathies and is especially important in light-chain diseases, such as light-chain myeloma, primary systemic amyloidosis, and light-chain-deposition disease. These diseases are more prevalent in...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:The Dutch project "Calibration 2000" aims at harmonization of laboratory results via calibration by development of matrix-based secondary reference materials. We considered the selection, preparation, and characterization of 34 potential reference materials (PRMs). METHODS:Sixteen PRMs were prepared either ...
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:
更新日期:2002-09-01 00:00:00
abstract:BACKGROUND:Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays to...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:KLK5 is a newly discovered human kallikrein gene. Many kallikrein genes have been found to be differentially expressed in various malignancies, and prostate-specific antigen (PSA; encoded by the KLK3 gene) is the best tumor marker for prostate cancer. Like the genes that encode PSA and other kallikreins, the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:In malignancy, gene mutations frequently occur in tumor suppressor genes such as p53 and are sporadically located. We describe a homogeneous method for amplification and mutation scanning, and apply the method to the p53 gene. METHODS:Using a series of overlapping fluorescein-labeled oligonucleotides comple...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-08-01 00:00:00
abstract:BACKGROUND:At concentrations <500 microg/L, serum alpha-fetoprotein (AFP) has low specificity in the diagnosis of hepatocellular carcinoma (HCC), but monosialylated AFP (msAFP) is more specific for HCC. We describe two strategies for quantitative analysis of msAFP and explore their diagnostic accuracy in cases of HCC w...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:Plasma creatinine concentration and calculated creatinine clearance are of limited value as glomerular filtration rate (GFR) markers in patients with decompensated liver cirrhosis. We assessed plasma cystatin C as an indicator of GFR in such patients. METHODS:We studied 36 patients with decompensated liver ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND:Because reference values for vitamin B(12) concentrations and vitamin B(12)-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B(12) status. The aim of the present study was to describe ranges of biochemical indices ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract:BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the feasibility of infrared (IR) spectroscopy for the simultaneous quantification of serum LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) concentrations. METHODS:Serum samples (n = 90) were obtained. Duplicate aliquots (5 microL) of the serum specimens were dried...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:The "unit" for "enzymic activity" (U = 1 micromol/min) was recommended by the International Union of Biochemistry and Molecular Biology (IUB) in 1961 and is widely used in medical laboratory reports. The general trend in metrology, however, is toward global standardization through defining units coherent wit...
journal_title:Clinical chemistry
pub_type: 历史文章,杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Apolipoprotein (apo) E is a component of two major classes of plasma lipoproteins, apo B- (apo E-LpB) and non-apo B-containing (apo E-Lp-non-B) lipoproteins. The factors that affect total apo E in particles [lipoprotein E (LpE), apo E-Lp-non-B, and apo E-LpB], are incompletely characterized. METHODS:We stud...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00