Abstract：
BACKGROUND:An abnormal IgLkappa:IgLlambda ratio has long been used as a clinical criterion for non-Hodgkin B-cell lymphomas. As a first step toward a quantitative real-time PCR-based multimarker diagnostic analysis of lymphomas, we have developed a method for determination of IgLkappa:IgLlambda ratio in clinical samples. METHODS:Light-up probe-based real-time PCR was used to quantify IgLkappa and IgLlambda cDNA from 32 clinical samples. The samples were also investigated by routine immunohistochemical analysis and flow cytometry. RESULTS:Of 32 suspected non-Hodgkin lymphoma samples analyzed, 28 were correctly assigned from real-time PCR measurements assuming invariant PCR efficiencies in the biological samples. Four samples were false negatives. One was a T-cell lymphoma, one was a diffuse large B-cell lymphoma, and one was reanalyzed and found lymphoma-positive by in situ calibration, which takes into account sample-specific PCR inhibition. Twelve of the samples were fine-needle aspirates, and these were all correctly assigned. CONCLUSIONS:This work is a first step toward analyzing clinical samples by quantitative light-up probe-based real-time PCR. Quantitative real-time PCR appears suitable for high-throughput testing of cancers by measuring expression of tumor markers in fine-needle aspirates.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Ståhlberg A,Aman P,Ridell B,Mostad P,Kubista Mdoi
10.1373/49.1.51subject
Has Abstractpub_date
2003-01-01 00:00:00pages
51-9issue
1eissn
0009-9147issn
1530-8561journal_volume
49pub_type
杂志文章abstract：BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...
journal_title：Clinical chemistry
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doi：10.1373/49.6.935
更新日期：2003-06-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1980-10-01 00:00:00
abstract：:alpha 1-Antitrypsin (A1AT) deficiency is a relatively common autosomal recessive disease, resulting most often from a single base pair (1 bp) substitution called the Z mutation. Previous genetic tests for carriers and affected patients have relied on quantitative binding of radioactive probes to an amplified gene prod...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nea...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：BACKGROUND:Endothelial nitric oxide synthase (eNOS) could be a candidate gene for coronary artery disease (CAD). This study investigated the relationship of the eNOS Glu(298)-->Asp and T(786)-->C polymorphisms with the presence and severity of CAD in the Italian population. METHODS:We enrolled 415 unrelated individual...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/49.3.389
更新日期：2003-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1999-12-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1994-11-01 00:00:00
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journal_title：Clinical chemistry
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doi：10.1373/clinchem.2016.268698
更新日期：2017-08-01 00:00:00
abstract：:Parathyroid hormone-related protein (PTHrP) was measured in plasma by a new immunoradiometric assay (IRMA) from Nichols Institute. The assay is specific for PTHrP and shows excellent parallelism when measuring keratinocyte fluid, samples with high PTHrP content, and PTHrP-supplemented plasma. A precision profile estab...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.119396
更新日期：2009-10-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:We describe a liquid-chromatographic assay for AZT in human plasma and urine. This assay involves the use of two internal standards, allowing reference of AZT peaks to the appropriate internal standard, the choice depending on the range of concentrations encountered. This method is isocratic, specific, sensitive enoug...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:The herbicide paraquat (1,1'-dimethyl-4,4'-bipyridylium dichloride; PQ), is a poison known to cause delayed mortality due to lung and kidney injuries. High-resolution proton nuclear magnetic resonance (1H NMR) spectroscopy has been extensively applied in evaluating nephrotoxicity by the characteristic perturbations in...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1998-06-01 00:00:00
abstract：:We review the following labels used as substitutes for radioisotopes in immunoassay systems: bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performi...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1981-07-01 00:00:00
abstract：:Measurement of acetaminophen by analysis of the second derivative of its spectrum is specific and sensitive. The method of extraction and the use of just one phosphate buffer as reagent makes this method very convenient. Readings are reliable from 10 to 1500 mg/L. A turnaround time of 20 min makes this method well sui...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values fo...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
abstract：:A simple computer-based screening technique has been developed for classifying human expired air components into 16 chemical classes, based on empirical formulas. The sort procedure was developed to simplify the screening of the composition of expired air samples by sorting all components into chemical classes and cla...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Direct equilibrium dialysis and direct ultrafiltration free thyroxine (T(4)) assays rely on semipermeable membranes to exclude T(4)-binding serum proteins from dialysates and ultrafiltrates. The presence of these proteins in dialysates or ultrafiltrates will yield spuriously high free T(4) values when free T...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.085316
更新日期：2007-05-01 00:00:00
abstract：:Three groups of 10 age- and sex-matched nondiabetic volunteers took 0, 750, or 1500 mg of vitamin C each day for 12 weeks. Glycohemoglobin (GHb) was measured by HPLC, electrophoresis, affinity chromatography, and immunoassay at baseline (-4 weeks and -1 day), during supplementation (6 weeks and 12 weeks), and after su...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:This rapid radioimmunoassay for aldosterone is performed directly on 100 microL of unprocessed plasma, with 125I-labeled aldosterone as the labeled antigen. Our use of steroid-free plasma in preparing the standard curve resulted in an overestimate of aldosterone; this problem was overcome by adding to such plasma a mi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：:We used gas chromatography-mass spectrometry to analyze serum specimens from a patient whose metabolic ketoacidosis resulted from sniffing a toluene-containing substance; we also analyzed a sample of the abused substance itself and a serum specimen from a normal subject. The blood samples from the patient showed abnor...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:We compared the activities of thyroid-stimulating antibodies (TSAb) measured in cultures of human thyrocytes and the values for thyrotropin-receptor antibodies (TRAb) as measured with a commercial kit based on use of radiolabeled receptors. Sera were obtained from patients with Graves' disease before, during, and afte...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.132480
更新日期：2010-03-01 00:00:00
abstract：:We describe a rapid polarization fluoroimmunoassay for screening for the presence of barbiturates in urine. The single reagent is prepared by pre-mixing antiserum with a fluorescein-labeled barbiturate derivative as tracer. Use of a mixture of two sheep antisera, raised against different barbiturate immunogens, result...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:A new, highly sensitive immunoradiometric thyrotropin (TSH) assay involving solid-phase-coupled monoclonal antibodies (Boots-Celltech Sucrosep IRMA-TSH) has been evaluated in a wide variety of patients with thyroidal and nonthyroidal illnesses and the results compared with those obtained by conventional diagnostic TSH...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-03-01 00:00:00
abstract：:The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the cal...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
abstract：BACKGROUND:Concerns exist regarding how the 99th percentile upper reference limit (URL) of cardiac troponin (cTn) is determined and whether it should be derived from normal healthy individuals. CONTENT:The 99th percentile URL of cTn is an important criterion to standardize the diagnosis of myocardial infarction (MI) f...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1093/clinchem/hvaa158
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2003.016311
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identi...
journal_title：Clinical chemistry
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更新日期：2015-01-01 00:00:00
