Abstract:
BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT exists as 2 isoforms: IRT1 and IRT2, with IRT2 being more closely aligned with pancreatic disease, including CF. Assay standardization between programs is a continuing problem because the IRT assays currently in use variously recognize either 1 or both isoforms. Here we report the development of a multiplexed assay for both forms of IRT simultaneously. METHODS:Using 2 different Luminex bead sets, we developed assays for each IRT isoform separately and then combined them. Using the sum of IRT1 and IRT2 values (IRT1+IRT2), we compared the results with a CF kit currently in use. RESULTS:In a sample set consisting of 16 cases confirmed positive for CF, we established a cutoff at >97 microg/L total IRT. Seven of 8 carriers with 1 CF mutation screen-positive by the standard method were also screen-positive by IRT1+IRT2. Of 32 cases screen-positive by standard IRT, 11 were screen-negative by IRT1+IRT2. None of these 11 cases had CF mutations identified by the screening program. CONCLUSIONS:These data indicate that the multiplex method with specificity for 2 isoforms of IRT has performance comparable to that of a standard IRT method and the advantage of improved standardization by detection of the 2 isoforms.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Lindau-Shepard BA,Pass KAdoi
10.1373/clinchem.2009.132480subject
Has Abstractpub_date
2010-03-01 00:00:00pages
445-50issue
3eissn
0009-9147issn
1530-8561pii
clinchem.2009.132480journal_volume
56pub_type
杂志文章abstract:INTRODUCTION:Commercially available SARS-CoV-2 serological assays based on different viral antigens have been approved for the qualitative determination of anti-SARS-CoV-2 antibodies. However, there are limited published data associating the results from commercial assays with neutralizing antibodies. METHODS:67 speci...
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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更新日期:2008-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1977-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:2014-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章,多中心研究
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.079012
更新日期:2007-04-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND:Bilirubin has antioxidative and cytoprotective properties. Low plasma concentrations of bilirubin are reportedly associated with the development of coronary and cerebrovascular disease, and bilirubin concentrations are strongly correlated with the enzyme activity of the hepatic uridine diphosphate glucuronos...
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pub_type: 临床试验,杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-11-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2003-04-01 00:00:00
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更新日期:2006-01-01 00:00:00
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doi:
更新日期:1997-08-01 00:00:00
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doi:
更新日期:2002-06-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1977-09-01 00:00:00
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doi:
更新日期:1976-02-01 00:00:00
abstract::This highly sensitive method for determination of beta 2-microglobulin (beta 2-m) in human urine or serum is based on direct agglutination by beta 2-m of latex particles on which an antibody against beta 2-m is adsorbed. The agglutination is quantified by counting the remaining unagglutinated particles, or by turbidim...
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doi:
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journal_title:Clinical chemistry
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