Abstract：
:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin immunoassays; assessed their ability to inhibit Na,K-ATPase catalytic activity; and measured their binding to proteins in serum. As assayed with ACS:180, Stratus, RIA, On-Line, and TDx digoxin assays, oleandrin at 100 micromol/L in digoxin-free serum gave apparent digoxin values of 0, 0.83, 2.24, 2.37, and 5.34 nmol/L, respectively, whereas oleandrigenin at that concentration gave results of 0, 0.52, 0.77, 4.94, and 1.40 nmol/L. Study of Na,K-ATPase inhibition showed IC50 values (micromol/L) of 0.22 for ouabain, 0.62 for oleandrin, 1.23 for oleandrigenin, and 2.69 for digoxin. At 25 degrees C, 96% of oleandrin and 48% of oleandrigenin were bound to serum proteins. Because detection of oleandrin and oleandrigenin by digoxin immunoassays is variable between assays as well as between congeners, assessment of cross-reactivity is warranted for each assay. The inhibition of Na,K-ATPase by oleandrin and oleandrigenin confirms that they likely exert their toxic effects through inhibition of sodium pump activity. In cases of digitalis-like poisoning with suspicion of oleander ingestion, a combination of digoxin immunoassays may be useful to effectively rule out the presence of oleander.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Jortani SA,Helm RA,Valdes R Jrsubject
Has Abstractpub_date
1996-10-01 00:00:00pages
1654-8issue
10eissn
0009-9147issn
1530-8561journal_volume
42pub_type
杂志文章abstract：BACKGROUND:Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/49.6.861
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1991-10-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-12-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1985-07-01 00:00:00
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doi：10.1373/clinchem.2004.047399
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journal_title：Clinical chemistry
pub_type： 共识发展会议,杂志文章,评审
doi：
更新日期：1990-08-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1997-07-01 00:00:00
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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doi：
更新日期：1993-10-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2005-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-02-01 00:00:00
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更新日期：2010-02-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.059568
更新日期：2006-02-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-10-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1998-01-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：1994-07-01 00:00:00
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doi：
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doi：
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
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doi：
更新日期：1983-07-01 00:00:00
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pub_type： 杂志文章,meta分析
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更新日期：2019-02-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-03-01 00:00:00
