Single-reagent polarization fluoroimmunoassay for amphetamine in urine.

abstract：:We have assessed the technical performance and robustness of NycoCard CRP Whole Blood, a near-patient test for C-reactive protein (CRP), when used in realistic daily routine situations in general practice clinics (GPC). Thirteen GPCs participated, five of them with technician staff. From 898 patients, split-sample mea...

journal_title：Clinical chemistry

authors： Dahler-Eriksen BS,Lassen JF,Petersen PH,Lund ED,Lauritzen T,Brandslund I

更新日期：1997-11-01 00:00:00

abstract：BACKGROUND:Tacrolimus is metabolized predominantly to 13-O-demethyltacrolimus in the liver and intestine by cytochrome P450 3A (CYP3A). Patients with high concentrations of CYP3A5, a CYP3A isoenzyme polymorphically produced in these organs, require higher doses of tacrolimus, but the exact mechanism of this association...

journal_title：Clinical chemistry

authors： Kamdem LK,Streit F,Zanger UM,Brockmöller J,Oellerich M,Armstrong VW,Wojnowski L

更新日期：2005-08-01 00:00:00

abstract：:Single-strand conformation polymorphism (SSCP) analysis is widely used to prescreen mutations in p53 gene. However, standardization of SSCP to detect p53 mutations has rarely been pursued so far. We have developed complete conditions for a temperature-controlled nonradioactive SSCP for mutation detection in amplified ...

journal_title：Clinical chemistry

authors： Welsh JA,Castrén K,Vähäkangas KH

更新日期：1997-12-01 00:00:00

abstract：:A liquid-chromatographic technique with a post-column enzymatic reaction and fluorescence detection was validated for analysis of individual bile acids in the serum of rats. Extraction recoveries averaged 91.1% (SD 6.9%) for all bile acids. The assay was sensitive (minimum detection of 16.8 pmol per 100-microL injecti...

journal_title：Clinical chemistry

authors： Thompson MB,Blair PC,Morris RW,Neptun DA,Deyo DF,Popp JA

更新日期：1987-10-01 00:00:00

abstract：:Peroxidation of lipids is believed to play a key role in various degenerative diseases. However, few simple tests are able to detect individual susceptibility or resistance to peroxidation. Measurement of the basal concentrations of lipid peroxides in plasma is not satisfactory because they are so low. Therefore, we d...

journal_title：Clinical chemistry

authors： Arshad MA,Bhadra S,Cohen RM,Subbiah MT

更新日期：1991-10-01 00:00:00

abstract：:We divided patients with brain lesions into three groups: (a) patients with primary or metastatic brain cancer, (b) brain infarctions, and (c) brain contusion(s). We analyzed each patient's sera for creatine kinase isoenzyme BB (CK-BB), using a monoclonal antibody kit (Impres-BB; International Immunoassay Laboratories...

journal_title：Clinical chemistry

authors： Schwartz JG,Bazan C 3rd,Gage CL,Prihoda TJ,Gillham SL

更新日期：1989-04-01 00:00:00

abstract：:To evaluate a theoretical model for calculating free thyroid hormones, based on the law of mass action, we compared values for both calculated and measured free thyroxin and free triiodothyronine in a group of normal subjects. To determine whether the concentrations of circulating free hormones were also predictable f...

journal_title：Clinical chemistry

authors： Fresco G,Curti G,Biggi A,Fontana B

更新日期：1982-06-01 00:00:00

abstract：BACKGROUND:Recent publications have explored the possibility of using fingerprints to confirm drug use, but none has yet dealt with environmental contamination from fingertips. Here we explored the possibility of establishing an environmental cutoff for drug testing from a single fingerprint. METHODS:Fingerprint sampl...

journal_title：Clinical chemistry

authors： Ismail M,Stevenson D,Costa C,Webb R,de Puit M,Bailey M

更新日期：2018-06-01 00:00:00

abstract：:To determine the molar absorptivities of beta-NADH and beta-NAD at 260 nm, we purified the reduced form of the coenzyme by repeated anion-exchange chromatography. A NADH preparation was so obtained for which the 260 nm/340 nm absorbance ratio was 2.265. When calculated with epsilon 340 beta-NADH = 6.22 times 10-3 for ...

journal_title：Clinical chemistry

authors： Haid E,Lehmann P,Ziegenhorn J

更新日期：1975-06-01 00:00:00

abstract：BACKGROUND:The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. Its diagnosis is based on clinical and laboratory criteria, defined by strict guidelines. The original clinical and laboratory criteria for the identification of APS patients were publi...

journal_title：Clinical chemistry

authors： Devreese K,Hoylaerts MF

更新日期：2010-06-01 00:00:00

abstract：:We describe a rapid, precise, and sensitive radiometric assay for human lactoferrin. In this typical "sandwich"-type assay, anti-human lactoferrin is adsorbed onto a polystyrene sphere and bound lactoferrin is detected by the subsequent binding of 125I-labeled anti-human lactoferrin. The assay is accurate for lactofer...

journal_title：Clinical chemistry

authors： Freeman KL,Anderson DC,Hughes B,Buffone GJ

更新日期：1985-03-01 00:00:00

abstract：:Zinc protoporphyrin is the predominent fluorescent porphyrin accumulating in erythrocytes as a result of chronic lead absorption or iron-deficiency anemia. Although diagnostic concentrations or thresholds for it in erythrocytes have been inferred, normal adult averages have not been established. We quantitatively asse...

journal_title：Clinical chemistry

authors： Zygowicz ER,Hollebone BR,Perkins HM

更新日期：1980-09-01 00:00:00

abstract：:We have designed a re-usable dialysis cell and a complex dialysis buffer, with which undiluted serum samples can be dialyzed with minimal changes in their serum matrix. Dialysate thyroxin (free T4) is then measured by a sensitive RIA for T4. The range of reportability was 2-128 ng/L, the normal range was 8-27 ng/L, an...

journal_title：Clinical chemistry

authors： Nelson JC,Tomei RT

更新日期：1988-09-01 00:00:00

abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：:We report the adaptation of four radioassays to the prototype of an automated radioimmunoassay system ("Centria," Union Carbide). The system consists of three integrated modules: (a) an automated pipettor, which dispenses samples and reagents; (b) the key module, an incubator/separator, in which centrifugal force is u...

journal_title：Clinical chemistry

authors： Ertingshausen G,Shapiro SI,Green G,Zborowski G

更新日期：1975-08-01 00:00:00

abstract：BACKGROUND:The kidney performs a multitude of essential functions to maintain homeostasis. In clinical medicine, glomerular filtration rate (GFR) provides the best index of overall kidney function, and proteinuria adds additional information on renal and nonrenal prognosis. Several novel biomarkers of kidney injury and...

journal_title：Clinical chemistry

authors： Ferguson MA,Waikar SS

更新日期：2012-04-01 00:00:00

abstract：:Reference values were determined for 23 plasma free amino acids from measurements done in 148 healthy children ranging from 0 to 18 years of age. Amino acid analysis was performed by ion-exchange chromatography. We propose a graphic form of presenting the age-specific distribution of plasma amino acid concentrations w...

journal_title：Clinical chemistry

authors： Lepage N,McDonald N,Dallaire L,Lambert M

更新日期：1997-12-01 00:00:00

abstract：:We devised a method for assaying serum lactate dehydrogenase isoenzyme 1 (LD-1) activity specifically by preincubation with alpha-chymotrypsin and guanidine. Cleavage of phenylalanine bonds in the loop of A and B subunits of LD-3, LD-4, and LD-5 isoenzymes (residues 117-119) by incubation with alpha-chymotrypsin for a...

journal_title：Clinical chemistry

authors： Shirahase Y,Watazu Y,Kaneda N,Uji Y,Okabe H,Karmen A

更新日期：1992-11-01 00:00:00

abstract：BACKGROUND:Cystic fibrosis is a life-threatening genetic disorder that has been associated with mutations in the CFTR [cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)] gene. Hundreds of CFTR mutations have been detected to date. Current CFTR genotyping assays target a s...

journal_title：Clinical chemistry

authors： Abou Tayoun AN,Tunkey CD,Pugh TJ,Ross T,Shah M,Lee CC,Harkins TT,Wells WA,Tafe LJ,Amos CI,Tsongalis GJ

更新日期：2013-10-01 00:00:00

abstract：:Investigating the feasibility and validity of determining plasma ferritin concentration in blood obtained by finger prick, we studied 29 adults (ages 21-49 years) and 35 children (ages 14-66 months). Blood was sampled simultaneously in the same subject from both the antecubital vein (venous blood) and by finger pricki...

journal_title：Clinical chemistry

authors： Mejía LA,Viteri FE

更新日期：1983-05-01 00:00:00

abstract：BACKGROUND:Increased concentrations of cell-free DNA have been found in several disorders and have been interpreted as evidence of increased rates of cell death or turnover. Evidence from in vitro and animal experiments suggests that DNA may play a role in the pathogenesis of rheumatoid arthritis (RA). METHODS:We meas...

journal_title：Clinical chemistry

authors： Zhong XY,von Mühlenen I,Li Y,Kang A,Gupta AK,Tyndall A,Holzgreve W,Hahn S,Hasler P

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:Although lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) levels are associated with cardiovascular events, Lp-PLA(2) is physically linked to LDL cholesterol (LDL-C). Whether measures of Lp-PLA(2) mass or activity continue to predict risk after LDL-C reduction by statin therapy is uncertain. METHODS:Lp...

journal_title：Clinical chemistry

authors： Ridker PM,MacFadyen JG,Wolfert RL,Koenig W

更新日期：2012-05-01 00:00:00

abstract：:A new method (enzyme-ligand immunoassay, ELIA) is described for the estimation of estrogen (ER) and progesterone (PR) receptors in microsamples of human breast cancer tissue. The technique, based on the nonisotopic measurement of receptor-bound estradiol and progesterone, involves three steps: (a) simultaneous saturat...

journal_title：Clinical chemistry

authors： Krambovitis E,Hatzidakis G,Hatzoglou A,Romain S,Durand A,Stefanakis A,Castanas E

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:Microalbuminuria (MA) is recognized as an important risk factor for cardiovascular and renal complications in diabetes. We sought to evaluate how screening for MA is conducted and how urine albumin (UA) results are interpreted in primary care internationally. METHODS:General practitioners (GPs) received a c...

journal_title：Clinical chemistry

authors： Aakre KM,Thue G,Subramaniam-Haavik S,Bukve T,Morris H,Müller M,Lovrencic MV,Plum I,Kallion K,Aab A,Kutt M,Gillery P,Schneider N,Horvath AR,Onody R,Oosterhuis W,Ricos C,Perich C,Nordin G,Sandberg S

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:Age-adjusted morbidity and mortality rates from coronary heart disease (CHD) are higher in men than in women. Androgens are suspected to be responsible for the male disadvantage. The genomic effect of androgens is mediated by the androgen receptor (AR), which has a polymorphic CAG repeat in exon 1. The numbe...

journal_title：Clinical chemistry

authors： Hersberger M,Muntwyler J,Funke H,Marti-Jaun J,Schulte H,Assmann G,Lüscher TF,von Eckardstein A

更新日期：2005-07-01 00:00:00

abstract：BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...

journal_title：Clinical chemistry

authors： Grenache DG,Parvin CA,Gronowski AM

更新日期：2003-06-01 00:00:00

abstract：:The present assay was developed for quantifying midazolam in plasma of patients hospitalized in intensive-care units or undergoing anesthesia and receiving many other drugs as well. Plasma samples are alkalinized with NaOH and midazolam is extracted into n-hexane. The organic phase is evaporated and reconstituted in n...

journal_title：Clinical chemistry

authors： Ha HR,Funk B,Maitre PO,Zbinden AM,Follath F,Thomson DA

更新日期：1988-04-01 00:00:00

abstract：BACKGROUND:The bleeding time (BT) test predicts a higher bleeding complication rate in populations at risk for inherited or acquired platelet dysfunction, but it is of limited assistance in evaluating individual patients. There are no reports of clinical outcomes after discontinuation of the BT test. METHODS:Interview...

journal_title：Clinical chemistry

authors： Lehman CM,Blaylock RC,Alexander DP,Rodgers GM

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:KLK5 is a newly discovered human kallikrein gene. Many kallikrein genes have been found to be differentially expressed in various malignancies, and prostate-specific antigen (PSA; encoded by the KLK3 gene) is the best tumor marker for prostate cancer. Like the genes that encode PSA and other kallikreins, the...

journal_title：Clinical chemistry

authors： Yousef GM,Scorilas A,Kyriakopoulou LG,Rendl L,Diamandis M,Ponzone R,Biglia N,Giai M,Roagna R,Sismondi P,Diamandis EP

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:The applicability of microarray-based transcriptome massive analysis is often limited by the need for large amounts of high-quality RNA. RNA arbitrarily primed PCR (RAP-PCR) is an unbiased fingerprinting PCR technique that reduces both the amount of initial material needed and the complexity of the transcrip...

journal_title：Clinical chemistry

authors： Grau M,Solé X,Obrador A,Tarafa G,Vendrell E,Valls J,Moreno V,Peinado MA,Capellá G

更新日期：2005-01-01 00:00:00