Molar absorptivities of beta-NADH and beta-NAD at 260 nm.

Abstract:

:To determine the molar absorptivities of beta-NADH and beta-NAD at 260 nm, we purified the reduced form of the coenzyme by repeated anion-exchange chromatography. A NADH preparation was so obtained for which the 260 nm/340 nm absorbance ratio was 2.265. When calculated with epsilon 340 beta-NADH = 6.22 times 10-3 for beta-NADH at 260 nm and 25 degrees C, a molar absorptivity of 14.1 times 10-3 liter - mol minus 1 - cm minus 1 resulted from this quotient. By use of the lactate dehydrogenase or glycerol-3-phosphate dehydrogenase assay, respectively, and referring to the new absorption coefficient for NADH at 260 nm, the molar absorptivity of beta-NAD at 260 nm and 25 degrees C was established to be 17.4 times 10-3 liter - mol minus 1 - cm minus 1. The values observed are lower than those reported thus far.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Haid E,Lehmann P,Ziegenhorn J

subject

Has Abstract

pub_date

1975-06-01 00:00:00

pages

884-7

issue

7

eissn

0009-9147

issn

1530-8561

journal_volume

21

pub_type

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