Plasma lipoprotein peroxidation potential: a test to evaluate individual susceptibility to peroxidation.

abstract：:Serum amyloid A (SAA), a sensitive acute-phase protein, is the precursor of AA fibrils in reactive amyloidosis. However, SAA is poorly immunogenic, and development and standardization of immunoassays of this protein have been difficult. We established an automated polyclonal/monoclonal microparticle capture enzyme imm...

journal_title：Clinical chemistry

authors： Wilkins J,Gallimore JR,Tennent GA,Hawkins PN,Limburg PC,van Rijswijk MH,Moore EG,Pepys MB

更新日期：1994-07-01 00:00:00

abstract：BACKGROUND:We used a difference in bias approach to evaluate the commutability of 4 frozen serum pools for 8 direct methods for measurement of HDL and LDL cholesterol (HDLC and LDLC). METHODS:Freshly collected nonfrozen sera from 138 diseased and 37 nondiseased patients and 4 frozen pools from the CDC Lipid Standardiz...

journal_title：Clinical chemistry

authors： Korzun WJ,Nilsson G,Bachmann LM,Myers GL,Sakurabayashi I,Nakajima K,Nakamura M,Shamburek RD,Remaley AT,Miller WG

更新日期：2015-08-01 00:00:00

abstract：:Abnormal creatine kinase (CK) isoenzyme patterns were observed in the serum of a 64-year-old woman with severe heart disease. Agarose electrophoresis revealed the presence of all the usual CK isoenzymes (MM, MB, and BB) plus an extra band between MM and MB. Total serum CK activity was within the normal range. Within 2...

journal_title：Clinical chemistry

authors： Csako G,Papadopoulos NM,Jett GK,McIntosh CL

更新日期：1982-10-01 00:00:00

abstract：BACKGROUND:Hypoxic ischemic encephalopathy (HIE) is a major cause of permanent neurological disabilities in full-term newborns. We measured activin A in urine collected immediately after birth in asphyxiated full-term newborns, and assessed the ability of the measurements to predict the occurrence of perinatal encephal...

journal_title：Clinical chemistry

authors： Florio P,Luisi S,Moataza B,Torricelli M,Iman I,Hala M,Hanna A,Petraglia F,Gazzolo D

更新日期：2007-03-01 00:00:00

abstract：:Inherited qualitative abnormalities of fibrinogen have been documented in more than 100 families. These dysfibrinogenemias usually are clinically silent, but in some cases are associated with bleeding, thrombosis, or defective wound healing. Abnormalities of the fibrinogen molecule may impair any of the major steps in...

journal_title：Clinical chemistry

authors： Bithell TC

更新日期：1985-04-01 00:00:00

abstract：BACKGROUND:Carcinoembryonic antigen (CEA) is one of the most widely used tumor markers worldwide. Its main application is mostly in gastrointestinal cancers, especially in colorectal malignancy. Although in use for almost 30 years, the clinical value of CEA in colorectal cancer is still not clear. METHODS:The literatu...

journal_title：Clinical chemistry

authors： Duffy MJ

更新日期：2001-04-01 00:00:00

abstract：BACKGROUND:Multiple genes that are associated with the risk of developing diabetes or the risk of diabetes complications have been identified by candidate gene analysis and genomewide scanning. These molecular markers, together with clinical data and findings from proteomics, metabolomics, pharmacogenetics, and other m...

journal_title：Clinical chemistry

authors： Malandrino N,Smith RJ

更新日期：2011-02-01 00:00:00

abstract：:The identification of nicotinamide-N1-oxide as a metabolite in the urine of a schizophrenic patient prompted a study of the relative metabolism of nicotinic acid and nicotinamide in mental patients and healthy volunteers. Metabolites quantified included N1-methyl-2-pyridone-5-carboxamide, N1-methyl-4-pyridone-3-carbox...

journal_title：Clinical chemistry

authors： Mrochek JE,Jolley RL,Young DS,Turner WJ

更新日期：1976-11-01 00:00:00

abstract：BACKGROUND:Hemoglobin-based oxygen carriers (HBOCs) are being developed as potential substitutes for the oxygen-carrying functions of erythrocytes, but athletes may obtain and experiment with HBOCs as an illicit means of enhancing oxygen transport. An electrophoretic technique has been developed to screen for the prese...

journal_title：Clinical chemistry

authors： Varlet-Marie E,Ashenden M,Lasne F,Sicart MT,Marion B,de Ceaurriz J,Audran M

更新日期：2004-04-01 00:00:00

abstract：:A "sandwich" enzyme immunoassay for ferritin in human serum is described, in which horseradish peroxidase-labeled antibody and a very sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazolin-6-sulfonate), are used. The solid phase is polypropylene test tubes treated with glutaraldehyde; immunologic reactions are devel...

journal_title：Clinical chemistry

authors： Revenant MC

更新日期：1983-04-01 00:00:00

abstract：:Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled ...

journal_title：Clinical chemistry

authors： Kienhuis CB,Heuvel JJ,Ross HA,Foekens JA,Benraad TJ

更新日期：1992-05-01 00:00:00

abstract：BACKGROUND:Recurrent pregnancy loss, (RPL) affecting 1%-2% of couples, is defined as ≥3 consecutive pregnancy losses before 20-week' gestation. Women with RPL are routinely screened for etiological factors, but routine screening of male partners is not currently recommended. Recently it has been suggested that sperm qu...

journal_title：Clinical chemistry

authors： Jayasena CN,Radia UK,Figueiredo M,Revill LF,Dimakopoulou A,Osagie M,Vessey W,Regan L,Rai R,Dhillo WS

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:Because cyclosporin A (CsA) and glucocorticoids inhibit the production of interleukin-2 (IL-2) and other cytokines, quantitative analysis of cytokine mRNA might constitute a pharmacodynamic measure for immunosuppressive drug effects. We investigated whether immunosuppressive drugs influence cytokine mRNA exp...

journal_title：Clinical chemistry

authors： Härtel C,Fricke L,Schumacher N,Kirchner H,Müller-Steinhardt M

更新日期：2002-12-01 00:00:00

abstract：:Five genetic defects have been established as risk factors for venous thrombosis. Three are protein C, protein S, and antithrombin deficiencies, defects in the anticoagulant pathways of blood coagulation. Together they can be found in approximately 15% of families with inherited thrombophilia. Their laboratory diagnos...

journal_title：Clinical chemistry

authors： Bertina RM

更新日期：1997-09-01 00:00:00

abstract：:We compared measurements of apoprotein A-I (apoA-I) in plasma and in heparin-MnCl2 supernates analyzed by radial immunodiffusion. The apoA-I values were similar when the samples were fresh [n = 41, mean (SD), mg/L: plasma, 1393.4(349); heparin-MnCl2 supernates, 1364.9(332), p less than 0.01], but were greater than 8% ...

journal_title：Clinical chemistry

authors： Jiang XR,Bachorik PS

更新日期：1986-06-01 00:00:00

abstract：BACKGROUND:Protein tyrosine phosphatase 1B (PTPN1) dephosphorylates insulin receptors and attenuates insulin signaling. Polymorphisms in the coding sequence of PTPN1 have been variably associated with type 2 diabetes (T2D). We hypothesized that variations within the PTPN1 promoter might contribute to the development of...

journal_title：Clinical chemistry

authors： Meshkani R,Taghikhani M,Al-Kateb H,Larijani B,Khatami S,Sidiropoulos GK,Hegele RA,Adeli K

更新日期：2007-09-01 00:00:00

abstract：:We describe an improved procedure for the preparation of plasma or serum for determination of theophylline by reverse phase high-performance liquid chromatography. Quantitative results are available in less than 30 min from receipt of sample. The chromatogram is complete in 8 to 16 min, which includes the use of an in...

journal_title：Clinical chemistry

authors： Nelson JW,Cordry AL,Aron CG,Bartell RA

更新日期：1977-01-01 00:00:00

abstract：:We have improved the protocol for RNA quantification by using RNase protection. Instead of precipitation and extraction with phenol and chloroform, we use a faster and more reliable precipitation based on guanidinium thiocyanate (GdSCN). The internal standard is produced by in vitro transcription of a DNA template con...

journal_title：Clinical chemistry

authors： Petersen NE,Larsen LK,Nissen H,Jensen LG,Jensen A,Hyltoft Petersen P,Hørder,Gregersen N,Kristiansen K

更新日期：1995-11-01 00:00:00

abstract：BACKGROUND:We have developed a silicon-based biosensor that generates a visual signal in response to nucleic acid targets. METHODS:In this system, capture oligonucleotide probes are immobilized on the surface of the biosensor. Interaction of the capture probes with a complementary target and a biotinylated detector ol...

journal_title：Clinical chemistry

authors： Ostroff RM,Hopkins D,Haeberli AB,Baouchi W,Polisky B

更新日期：1999-09-01 00:00:00

abstract：:Ammonia concentrations in plasma may increase because of contamination and deterioration of blood components during specimen handling and storage. Using replicate specimens from healthy volunteers, we studied influences of specimen processing and storage procedures on ammonia measurements made with a self-contained re...

journal_title：Clinical chemistry

authors： Howanitz JH,Howanitz PJ,Skrodzki CA,Iwanski JA

更新日期：1984-06-01 00:00:00

abstract：:Antiserum performance in a nephelometric system can be characterized by parameters derived from measuring reaction rates. The characterization process is derived from a series of dose-response curves (elicited nephelometric response vs antigen concentration) generated from various dilutions of the antiserum being test...

journal_title：Clinical chemistry

authors： Hudson GA,Ritchie RF,Haddow JE

更新日期：1981-11-01 00:00:00

abstract：:This new assay procedure for diaphorase eliminates problems of high blank rates and nonlinear kinetics associated with other methods. The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm. ...

journal_title：Clinical chemistry

authors： Boethling RS,Weaver TL

更新日期：1979-12-01 00:00:00

abstract：:A new, highly sensitive immunoradiometric thyrotropin (TSH) assay involving solid-phase-coupled monoclonal antibodies (Boots-Celltech Sucrosep IRMA-TSH) has been evaluated in a wide variety of patients with thyroidal and nonthyroidal illnesses and the results compared with those obtained by conventional diagnostic TSH...

journal_title：Clinical chemistry

authors： Bassett F,Eastman CJ,Ma G,Maberly GF,Smith HC

更新日期：1986-03-01 00:00:00

abstract：BACKGROUND:Plasma amino acid (PAA) profiles are used in routine clinical practice for the diagnosis and monitoring of inherited disorders of amino acid metabolism, organic acidemias, and urea cycle defects. Interpretation of PAA profiles is complex and requires substantial training and expertise to perform. Given previ...

journal_title：Clinical chemistry

authors： Wilkes EH,Emmett E,Beltran L,Woodward GM,Carling RS

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:New genetic forms of hereditary hemochromatosis (HH) or hereditary hyperferritinemia (HF) have been identified over the last few years, and abnormalities of various genes may interact in a single patient. This study aimed to develop a rapid automated method for sequencing the main genes involved. METHODS:We...

journal_title：Clinical chemistry

authors： Cunat S,Giansily-Blaizot M,Bismuth M,Blanc F,Dereure O,Larrey D,Quellec AL,Pouderoux P,Rose C,Raingeard I,Renard E,Schved JF,Aguilar-Martinez P,CHU Montpellier AOI 2004 Working Group.

更新日期：2007-12-01 00:00:00

abstract：:We describe conditions for determining carboxypeptidase N (EC 3.4.17.3) activity by liquid chromatography. Serum (10 microL) is mixed with the artificial substrates hippuryl-L-arginine (30 mmol/L) and hippuryl-L-lysine (100 mmol/L) in 50 mmol/L 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution...

journal_title：Clinical chemistry

authors： Hendriks D,Scharpé S,van Sande M

更新日期：1985-12-01 00:00:00

abstract：:We measured immunoglobulins in the sera of 33 patients on days 1, 3, 6, 10, and 17 and three to four weeks after surgical operations (mostly hysterectomy or appendectomy) or (six patients) after spinal injury. In the absence of infection or blood transfusion, IgG usually decreased slightly and transiently after hyster...

journal_title：Clinical chemistry

authors： Fuller JM,Keyser JW

更新日期：1975-05-01 00:00:00

abstract：BACKGROUND:Our previously developed assay for detection of the factor V Leiden mutation (G1691A) based on a nucleic acid photo-cross-linking technology used two allele-specific capture probes and six fluorescein-modified signal-generating reporter probes. We wished to improve the sensitivity and performance of the meth...

journal_title：Clinical chemistry

authors： French C,Li C,Strom C,Sun W,Van Atta R,Gonzalez B,Wood M

更新日期：2004-02-01 00:00:00

abstract：:A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After ...

journal_title：Clinical chemistry

authors： Rong H,Tørring O,Sääf M,Sjöstedt U,Sjöberg HE,Bucht E

更新日期：1994-09-01 00:00:00

abstract：BACKGROUND:A sensitive gas chromatography-negative ion chemical ionization mass spectrometry (GC/MS-NICI) method was developed and validated for the measurement of Delta(9)-tetrahydrocannabinol (THC) in human sweat patches. METHODS:THC-d(0) and THC-d(3) were added to worn blank sweat patches (PharmChek; PharmChem Inco...

journal_title：Clinical chemistry

authors： Saito T,Wtsadik A,Scheidweiler KB,Fortner N,Takeichi S,Huestis MA

更新日期：2004-11-01 00:00:00