Validated gas chromatographic-negative ion chemical ionization mass spectrometric method for delta(9)-tetrahydrocannabinol in sweat patches.

abstract：:We describe a time-resolved immunofluorometric assay (IFMA) for corticotropin in unextracted human plasma, based on the use of two monoclonal antibodies: europium-labeled antibody 1A12 and antibody 2A3 coated onto microtiter wells. We compared the results of this assay with those of an immunoradiometric assay (IRMA) p...

journal_title：Clinical chemistry

authors： Dobson S,White A,Hoadley M,Lovgren T,Ratcliffe J

更新日期：1987-10-01 00:00:00

abstract：:A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after ...

journal_title：Clinical chemistry

authors： Egberts J,Soederhuizen W,Gebhardt DO

更新日期：1983-02-01 00:00:00

abstract：BACKGROUND:Coffee consumption has been associated with several risk factors for coronary heart disease, including increased cholesterol, increased blood pressure, and increased plasma total homocysteine (tHcy). tHcy is determined by several B-vitamins. However, reports about the association between coffee intake and B-...

journal_title：Clinical chemistry

authors： Ulvik A,Vollset SE,Hoff G,Ueland PM

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:MALDI-TOF mass spectrometry (MS) is set to make inroads into clinical chemistry because it offers advantages over other analytical platforms. These advantages include low acquisition and operating costs, ease of use, ruggedness, and high throughput. When coupled with innovative front-end strategies and appli...

journal_title：Clinical chemistry

authors： Duncan MW,Nedelkov D,Walsh R,Hattan SJ

更新日期：2016-01-01 00:00:00

abstract：:Direct-injection analysis for phenylalanine in plasma by isocratic "high-performance" liquid chromatography (HPLC) is described. For chromatographic separation I use an internal-surface reversed-phase silica support. The method involves ultraviolet detection, requires as little as 10 microL of plasma (routinely 20 mic...

journal_title：Clinical chemistry

authors： Atherton ND

更新日期：1989-06-01 00:00:00

abstract：BACKGROUND:Iron may promote coronary atherosclerotic disease (CAD) by increasing lipid peroxidation. Studies on biochemical or genetic markers of body iron stores as risk factors for CAD have yielded conflicting results. METHODS:We studied 849 individuals with a clear-cut definition of the CAD phenotype, i.e., with (C...

journal_title：Clinical chemistry

authors： Bozzini C,Girelli D,Tinazzi E,Olivieri O,Stranieri C,Bassi A,Trabetti E,Faccini G,Pignatti PF,Corrocher R

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:New genetic forms of hereditary hemochromatosis (HH) or hereditary hyperferritinemia (HF) have been identified over the last few years, and abnormalities of various genes may interact in a single patient. This study aimed to develop a rapid automated method for sequencing the main genes involved. METHODS:We...

journal_title：Clinical chemistry

authors： Cunat S,Giansily-Blaizot M,Bismuth M,Blanc F,Dereure O,Larrey D,Quellec AL,Pouderoux P,Rose C,Raingeard I,Renard E,Schved JF,Aguilar-Martinez P,CHU Montpellier AOI 2004 Working Group.

更新日期：2007-12-01 00:00:00

abstract：:The core antigen component of hepatitis B virus (HBV), HBcAg, may be used as a marker of viral replication. We describe a radioimmunoassay for HBcAg in serum, and compare results with those by other similar methods. Samples are centrifuged over a sucrose gradient containing 2-mercaptoethanol, and the resulting pellets...

journal_title：Clinical chemistry

authors： Quiroga JA,González O,Carreño V,Mora I,Porres JC,Gutiez J,Hernández-Guío C

更新日期：1985-06-01 00:00:00

abstract：:We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wid...

journal_title：Clinical chemistry

authors： Wolf BA,Daft MC,Koenig JW,Flye MW,Turk JW,Scott MG

更新日期：1989-01-01 00:00:00

abstract：:We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tra...

journal_title：Clinical chemistry

authors： Duncan P,Gochman N,Bayse D,Burnett R,Carey N,Carter R,Fellows WD,Garber C,Kessler G,McComb R,Miller W,Nast P,Ryan W,Schaffer R,Tejeda B,Vanderlinde R,Widdowson G

更新日期：1982-02-01 00:00:00

abstract：:I describe an assay for serum estriol during pregnancy, which does not require prior extraction or chromatography of the serum. Fifty microliters of 20-fold diluted serum can be assayed to detect as little as 20 pg of estriol. For a 20 mug/liter serum estriol concentration the CV was 16%; for 100 mug/liter concentrati...

journal_title：Clinical chemistry

authors： Standefer J

更新日期：1976-04-01 00:00:00

abstract：:I present the general pattern relationship between (a) plasma fibrinogen and cholinesterase activity and (b) plasma fibrinogen and hemoglobin, in 250 and 310 hospitalized adults, respectively. Although responses of cholinesterase were often unrelated to fibrinogen, when its activity in plasma was depressed, above-norm...

journal_title：Clinical chemistry

authors： Rice EW

更新日期：1977-01-01 00:00:00

abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...

journal_title：Clinical chemistry

authors： Lindau-Shepard BA,Pass KA

更新日期：2010-03-01 00:00:00

abstract：:We report the results of a laboratory and clinical evaluation of a commercial kit procedure (Immuno Nuclear Corp.) for measuring the mid-region of the parathyrin molecule. The estimated dose at 50% binding averaged 130 pmol/L, and the minimum detectable concentration was 14 pmol/L. The within-assay CV was less than or...

journal_title：Clinical chemistry

authors： Haver VM,Rinker AD,Ko BM,Tietz NW

更新日期：1986-11-01 00:00:00

abstract：:Four double-monoclonal time-resolved immunofluorometric assays (TrIFMAs) have been developed for the specific determination of pregnancy-associated plasma protein A/proeosinophil major basic protein (PAPP-A/ proMBP) complex in first-trimester maternal serum samples. The assays have a functional sensitivity of < 4 mIU/...

journal_title：Clinical chemistry

authors： Qin QP,Christiansen M,Oxvig C,Pettersson K,Sottrup-Jensen L,Koch C,Nørgaard-Pedersen B

更新日期：1997-12-01 00:00:00

abstract：BACKGROUND:5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of 5ARD. MET...

journal_title：Clinical chemistry

authors： Chan AO,But BW,Lee CY,Lam YY,Ng KL,Tung JY,Kwan EY,Chan YK,Tsui TK,Lam AL,Tse WY,Cheung PT,Shek CC

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:Lectin-like oxidized LDL receptor 1 (LOX-1) is implicated in atherothrombotic diseases. Activation of LOX-1 in humans can be evaluated by use of the LOX index, obtained by multiplying the circulating concentration of LOX-1 ligands containing apolipoprotein B (LAB) times that of the soluble form of LOX-1 (sLO...

journal_title：Clinical chemistry

authors： Inoue N,Okamura T,Kokubo Y,Fujita Y,Sato Y,Nakanishi M,Yanagida K,Kakino A,Iwamoto S,Watanabe M,Ogura S,Otsui K,Matsuda H,Uchida K,Yoshimoto R,Sawamura T

更新日期：2010-04-01 00:00:00

abstract：BACKGROUND:Few data compare cardiac troponin T (cTnT) and cardiac troponin I (cTnI) in a general population. We sought to evaluate the distribution and association between cTnT, cTnI, and cardiovascular risk factors in a large general population cohort. METHODS:High-sensitivity cTnT and cTnI were measured in serum fro...

journal_title：Clinical chemistry

authors： Welsh P,Preiss D,Shah ASV,McAllister D,Briggs A,Boachie C,McConnachie A,Hayward C,Padmanabhan S,Welsh C,Woodward M,Campbell A,Porteous D,Mills NL,Sattar N

更新日期：2018-11-01 00:00:00

abstract：:To assess the distribution of serum concentrations of the atherogenic lipoprotein(a) [Lp(a)] in an obese population and the possible effects of weight reduction, we determined Lp(a) in 52 white, moderately obese subjects (16 men and 36 women). The subjects were participating in a weight-reduction program of diet, exer...

journal_title：Clinical chemistry

authors： Corsetti JP,Sterry JA,Sparks JD,Sparks CE,Weintraub M

更新日期：1991-07-01 00:00:00

abstract：:Erythrocyte protoporphyrin content is increased in several human and veterinary disorders in which heme synthesis is disrupted. The nature of the defect determines whether zinc protoporphyrin, unchelated (free) protoporphyrin, or both are present in the erythrocytes. Extraction of the protoporphyrin into acetone/water...

journal_title：Clinical chemistry

authors： Hart D,Piomelli S

更新日期：1981-02-01 00:00:00

abstract：BACKGROUND:The Dutch project "Calibration 2000" aims at harmonization of laboratory results via calibration by development of matrix-based secondary reference materials. We considered the selection, preparation, and characterization of 34 potential reference materials (PRMs). METHODS:Sixteen PRMs were prepared either ...

journal_title：Clinical chemistry

authors： Cobbaert C,Weykamp C,Baadenhuijsen H,Kuypers A,Lindemans J,Jansen R

更新日期：2002-09-01 00:00:00

abstract：:We have studied factors affecting biological variation in total plasma alkaline phosphatase in a population of 32 329 apparently healthy subjects four years old or older. Quantification of the bone and liver isoenzymes after thermal denaturation made it possible to specify the contributions of each isoenzyme to variat...

journal_title：Clinical chemistry

authors： Schiele F,Henny J,Hitz J,Petitclerc C,Gueguen R,Siest G

更新日期：1983-04-01 00:00:00

abstract：:In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted...

journal_title：Clinical chemistry

authors： Smith AM,Macdonald DJ

更新日期：1983-12-01 00:00:00

abstract：:A total of 1357 polypeptides [946 acidic (isoelectric focusing) and 411 basic (nonequilibrium pH-gradient electrophoresis)] from human HeLa cells have been separated and catalogued with use of high-resolution two-dimensional gel electrophoresis. Of these polypeptides, 1266 were detected by labeling cells with [35S]met...

journal_title：Clinical chemistry

authors： Bravo R,Celis JE

更新日期：1982-04-01 00:00:00

abstract：BACKGROUND:Single-nucleotide polymorphisms (SNPs) in inflammation-related genes have been linked to an increased risk of ischemic stroke. Most of these SNP results have not been replicated, however, and metaanalyses of the effects of inflammation-related genes are rare. We investigated 49 SNPs in 34 genes previously re...

journal_title：Clinical chemistry

authors： Greisenegger S,Zehetmayer S,Bauer P,Endler G,Ferrari J,Lang W,Janisiw M,Steiner L,Cheng S,Lalouschek W,Mannhalter C

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:The "unit" for "enzymic activity" (U = 1 micromol/min) was recommended by the International Union of Biochemistry and Molecular Biology (IUB) in 1961 and is widely used in medical laboratory reports. The general trend in metrology, however, is toward global standardization through defining units coherent wit...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：2002-03-01 00:00:00

abstract：BACKGROUND:The next-generation, high-sensitivity cardiac troponin assays can measure quantifiable concentrations of cTn in a majority of individuals, but there are few studies assessing these assays for risk stratification. The present study was undertaken to determine if a research hs-cTnI assay can be useful for pred...

journal_title：Clinical chemistry

authors： Kavsak PA,Wang X,Ko DT,MacRae AR,Jaffe AS

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:A substantial proportion of the variability in the absorption and clearance of cyclosporin A (CsA) after oral administration has been attributed to variability in liver cytochrome P-450 3A4 (CYP3A4) activity and intestinal P-glycoprotein (P-gp) concentration. A polymorphism in the CYP3A4 promoter region, ter...

journal_title：Clinical chemistry

authors： von Ahsen N,Richter M,Grupp C,Ringe B,Oellerich M,Armstrong VW

更新日期：2001-06-01 00:00:00

abstract：:By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values fo...

journal_title：Clinical chemistry

authors： Bernstein LH,Good IJ,Holtzman GI,Deaton ML,Babb J

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:Intravenous and subcutaneous microdialysis was performed to compare the free concentrations and pharmacokinetics of L-3, 4-dihyroxyphenylalanine (L-dopa) in blood and tissue in healthy subjects and in patients with Parkinson disease. METHODS:Nine healthy volunteers and 10 patients with Parkinson disease, st...

journal_title：Clinical chemistry

authors： Dizdar N,Kullman A,Norlander B,Olsson JE,Kagedal B

更新日期：1999-10-01 00:00:00