Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared.

abstract：:Comparison of two methods for determining a substance is a common procedure in clinical chemistry. Almost without exception, normal theory statistics are used with the tacit assumption that the data under test show gaussian distributions. Experience has led us to question this assumption for populations of differences...

journal_title：Clinical chemistry

authors： Wu GT,Twomey SL,Thiers RE

更新日期：1975-03-01 00:00:00

abstract：BACKGROUND:Low concentrations of cardiac troponin (cTn) have been recommended for rapid rule-out of acute myocardial infarction (AMI). We examined the Beckman Coulter Access high-sensitivity cardiac troponin I (hs-cTnI) assay to identify a single test threshold that can safely rule out AMI. METHODS:This analysis used ...

journal_title：Clinical chemistry

authors： Greenslade J,Cho E,Van Hise C,Hawkins T,Parsonage W,Ungerer J,Tate J,Pretorius C,Than M,Cullen L

更新日期：2018-05-01 00:00:00

abstract：:Problems encountered in the uroporphyrinogen I synthase assay were found to be due to the Triton reagent, slit-width and excitation wavelength settings of the spectrophotofluorometer, and leg-phase in the enzyme reaction. Of Triton X-100 from four suppliers, only three were found suitable. Differences in enzyme activi...

journal_title：Clinical chemistry

authors： Chamberlain BR,Buttery JE

更新日期：1980-08-01 00:00:00

abstract：:Currently, we can make the following generalizations about the major long-term complications of diabetes. First, they occur commonly after 10 to 15 years of diabetes. Second, the complications of renal and retinal disease, but not of atherogenic and neurological disease, appear to be related to the severity of hypergl...

journal_title：Clinical chemistry

authors： Santiago JV

更新日期：1986-10-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：:A potential genetic marker for hypertriglyceridemia and coronary disease, an apolipoprotein-CIII gene allele displaying a single nucleotide substitution, was found in 71% of Arabs with primary hypertriglyceridemia. Over 96% of the unrelated adults possessing this allele showed hypertriglyceridemia. The same allele was...

journal_title：Clinical chemistry

authors： Taş S

更新日期：1989-02-01 00:00:00

abstract：:For normal and above-normal concentrations of angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity in plasma, results of a manual fluorometric method [with hippuryl-histidyl-leucine (HHL), 5 mmol/L, as substrate] correlated well with those of an automated spectrophotometric method [with 3-(2-furylacryloyl)-L-phen...

journal_title：Clinical chemistry

authors： Gorski TP,Campbell DJ

更新日期：1991-08-01 00:00:00

abstract：:Creatine kinase (CK) isoenzymes MM, MB, and BB are located primarily in the cell cytosol, and increased CKMB in plasma is the hallmark of myocardial infarction. However, whether CK is released with reversible ischemic injury remains controversial. Here, we assessed plasma CK activity--cytosolic and mitochondrial CK--i...

journal_title：Clinical chemistry

authors： Ishikawa Y,Saffitz JE,Mealman TL,Grace AM,Roberts R

更新日期：1997-03-01 00:00:00

abstract：:To correct the results of a radioimmunoassay for beta-thyrotropin (TSH) subunit by eliminating the proportion ascribable to intact TSH, we have devised a method that experimentally reproduces the conditions under which the interference develops. Beta-TSH subunit was assayed in several preparations containing known con...

journal_title：Clinical chemistry

authors： Dotti C,Ceda GP,Denti L,Cavalieri S,Borasi G,Valenti G

更新日期：1987-05-01 00:00:00

abstract：:Lyophilized serum specimens were manufactured in such a way that fixed quantitative interspecimen relationships were established. The specimens were analyzed for about twenty constituents by several thousand laboratories in 1971, 1972, and 1973. Most of the consensus mean values derived from these analyses were found ...

journal_title：Clinical chemistry

authors： Grannis GF

更新日期：1976-07-01 00:00:00

abstract：:We have developed a sensitive procedure for determination of serum copper by use of the color reagent 4-(3,5-dibromo-2-pyridylazo)-N-ethyl-N-sulfopropylaniline. After mixing serum sample and reagent, and incubating at 37 degrees C for 5 min, we measure the absorbance of the resulting chelate complex at 580 nm (molar a...

journal_title：Clinical chemistry

authors： Abe A,Yamashita S,Noma A

更新日期：1989-04-01 00:00:00

abstract：BACKGROUND:Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnos...

journal_title：Clinical chemistry

authors： Kaleta EJ,Clark AE,Cherkaoui A,Wysocki VH,Ingram EL,Schrenzel J,Wolk DM

更新日期：2011-07-01 00:00:00

abstract：BACKGROUND:Identifying markers of chronic kidney disease (CKD) that occur early in the disease process and are specific to loss of kidney function rather than other underlying causes of disease may allow earlier, more accurate identification of patients who will develop CKD. We therefore sought to identify diagnostic b...

journal_title：Clinical chemistry

authors： Mohammadi-Shemirani P,Sjaarda J,Gerstein HC,Treleaven DJ,Walsh M,Mann JF,McQueen MJ,Hess S,Paré G

更新日期：2019-03-01 00:00:00

abstract：:Demonstrable quality of laboratory services entails two parts. First, one needs a quality policy statement, identification of user needs, choice of measurement procedures, reference measurement system to provide traceability, control materials, and proficiency testing with materials having reference-measurement-assign...

journal_title：Clinical chemistry

authors： Dybkaer R

更新日期：1994-07-01 00:00:00

abstract：:A radioimmunoassay for barbiturates is shown to be equally sensitive to phenobarbital and its major urinary metabolite, p-hydroxyphenobarbital, in serum and urine. Interference by the metabolite can be essentially eliminated by selectively extracting phenobarbital into chloroform. The extraction efficiency of the meth...

journal_title：Clinical chemistry

authors： Viswanathan CT,Booker HE,Welling PG

更新日期：1977-05-01 00:00:00

abstract：BACKGROUND:High circulating concentrations of N-terminal fragments of A- and B-type natriuretic peptides (NT-proANP and NT-proBNP) identify patients with impaired cardiac function. ProANP-derived peptides are particularly sensitive to increased preload of the heart and proBNP-derived peptides to increased afterload; th...

journal_title：Clinical chemistry

authors： Ala-Kopsala M,Ruskoaho H,Leppäluoto J,Seres L,Skoumal R,Toth M,Horkay F,Vuolteenaho O

更新日期：2005-04-01 00:00:00

abstract：BACKGROUND:HDL is quantitatively the most important lipoprotein in most species and mechanistic evidence points toward a role for HDL in normal immune function. We tested the hypothesis that concentrations of HDL cholesterol are associated with risk of autoimmune disease. METHODS:From 2 studies of the general populati...

journal_title：Clinical chemistry

authors： Madsen CM,Varbo A,Nordestgaard BG

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Coffee consumption has been associated with several risk factors for coronary heart disease, including increased cholesterol, increased blood pressure, and increased plasma total homocysteine (tHcy). tHcy is determined by several B-vitamins. However, reports about the association between coffee intake and B-...

journal_title：Clinical chemistry

authors： Ulvik A,Vollset SE,Hoff G,Ueland PM

更新日期：2008-09-01 00:00:00

abstract：:In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay ...

journal_title：Clinical chemistry

authors： Bugari G,Poiesi C,Beretta A,Ghielmi S,Albertini A

更新日期：1990-01-01 00:00:00

abstract：:Because of uncertainty as to the molecular weight of transferrin, a previous comparison [Von der Heul et al., Clin. Chim. Acta 38, 347 (1972)] between transferrin content of serum and total iron-binding capacity cannot be definitive. We found a conversion factor for expressing the maximum amount of iron bound by 1 mg ...

journal_title：Clinical chemistry

authors： Tsung SH,Rosenthal WA,Milewski KA

更新日期：1975-07-01 00:00:00

abstract：:Erythromycin, phenazopyridine, methenamine mandelate, acetazolamide, and ascorbic acid reportedly interfere with the Porter-Silber reaction in the determination of urinary 17-hydroxycorticosteroids. Sodium bisulfite, added to the urine after hydrolysis with glucuronidase, removed almost all nonsteroidal interferences ...

journal_title：Clinical chemistry

authors： Furuya E

更新日期：1977-11-01 00:00:00

abstract：:A precolumn derivatization method was optimized for rapid and specific analysis of total urinary hydroxyproline by HPLC. After an overnight hydrolysis, urine samples dried and reconstituted with the internal standard cysteic acid (in sodium hydrogen carbonate, pH 9.3) were derivatized with N,N-diethyl-2,4-dinitro-5-fl...

journal_title：Clinical chemistry

authors： Paroni R,De Vecchi E,Fermo I,Arcelloni C,Diomede L,Magni F,Bonini PA

更新日期：1992-03-01 00:00:00

abstract：:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct ...

journal_title：Clinical chemistry

authors： Peter F,Growcock G,Strunc G

更新日期：1978-09-01 00:00:00

abstract：BACKGROUND:Paraoxonase may mitigate oxidative damage and thus lower risk of macrovascular disease. METHODS:DNA samples from 2252 participants in the Coronary Artery Risk Development in Young Adults (CARDIA) study were genotyped for the L55M and Q192R polymorphisms of the PON1 (paraoxonase 1) gene, and paraoxonase acti...

journal_title：Clinical chemistry

authors： Thyagarajan B,Jacobs DR Jr,Carr JJ,Alozie O,Steffes MW,Kailash P,Hayes JH,Gross MD

更新日期：2008-04-01 00:00:00

abstract：BACKGROUND:Several compounds in the choline oxidation pathway are associated with insulin resistance and prevalent diabetes; however, prospective data are scarce.We explored the relationships between systemic and urinary choline-related metabolites and incident type 2 diabetes in an observational prospective study amon...

journal_title：Clinical chemistry

authors： Svingen GF,Schartum-Hansen H,Pedersen ER,Ueland PM,Tell GS,Mellgren G,Njølstad PR,Seifert R,Strand E,Karlsson T,Nygård O

更新日期：2016-05-01 00:00:00

abstract：:We evaluated and compared five commercial radioimmunoassay kits with a standard counter-immunoelectrophoretic assay for the measurement of prostatic acid phosphatase in serum. Four of the five radioimmunoassays performed as described by the supplier with respect to sensitivity, stability, precision, linearity, analyti...

journal_title：Clinical chemistry

authors： Wright GL Jr,Schellhammer PF,Brassil DN,Sieg SM,Leffell MS

更新日期：1981-10-01 00:00:00

abstract：:Hemoglobinometry according to the International Committee of Standardization in Hematology (ICSH) suffers from imprecision related to high sample dilution and from potential errors owing to sample turbidity. We have evaluated a new instrument, "HemoCue," that measures hemoglobin at two wavelengths as azide methemoglob...

journal_title：Clinical chemistry

authors： von Schenck H,Falkensson M,Lundberg B

更新日期：1986-03-01 00:00:00

abstract：:Each of 65 laboratories analyzed 10 whole-blood samples for erythrocyte protoporphyrin by one or more of several analytical procedures. These procedures were of two types: (a) extraction of protoporphyrin from the erythrocytes into ethyl acetate/acetic acid, re-extraction into hydrochloric acid, and fluorometric measu...

journal_title：Clinical chemistry

authors： Jackson KW

更新日期：1978-12-01 00:00:00

abstract：BACKGROUND:The goal of this study was to establish a plasma microRNA profile by use of next-generation sequencing that could aid in assessment of patient prognosis in nasopharyngeal carcinoma (NPC). METHODS:Two panels of NPC patients and healthy controls (HCs) were recruited for this study. We used deep sequencing to ...

journal_title：Clinical chemistry

authors： Wang HY,Yan LX,Shao Q,Fu S,Zhang ZC,Ye W,Zeng YX,Shao JY

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:Lead is an environmental pollutant, and human exposure is assessed by monitoring lead concentrations in blood. Because the main source of environmental exposure has been the use of leaded gasoline, its phase-out has led to decreased lead concentrations in the general population. Therefore, validated analytic...

journal_title：Clinical chemistry

authors： Patriarca M,Castelli M,Corsetti F,Menditto A

更新日期：2004-08-01 00:00:00