Evaluation of "HemoCue," a new device for determining hemoglobin.

abstract：:We evaluated superoxide dismutase (EC 1.15.1.1) and glutathione peroxidase (EC 1.11.1.9) activities in the erythrocytes of lead-exposed and nonexposed workers from distinct industries localized in the State of São Paulo, Brazil. Concentrations of either lead or protoporphyrin IX, or both, in blood were used to indicat...

journal_title：Clinical chemistry

authors： Monteiro HP,Abdalla DS,Arcuri AS,Bechara EJ

更新日期：1985-10-01 00:00:00

abstract：:This relatively simple, rapid method for quantification of homovanillic acid (HVA) and vanillylmandelic acid (VMA) involves solvent extraction and capillary gas chromatography. With use of this extraction method, the overall analytical recovery from an aqueous solution is 92.5% for HVA and 79.2% for VMA. 3,4-Dihydroxy...

journal_title：Clinical chemistry

authors： Tuchman M,Crippin PJ,Krivit W

更新日期：1983-05-01 00:00:00

abstract：:Considerable evidence has accrued in the last two decades to support the hypothesis that alterations in serotonergic neuronal function in the central nervous system occur in patients with major depression. These findings include the following: (a) reduced cerebrospinal fluid (CSF) concentrations of 5-hydroxyindoleacet...

journal_title：Clinical chemistry

authors： Owens MJ,Nemeroff CB

更新日期：1994-02-01 00:00:00

abstract：:We describe a liquid-chromatographic procedure for simultaneously determining eight steroids in serum. We used a Zorbax ODS column and a mobile phase of methanol/isopropanol/water (44/10/46, by vol), which well resolves the steroids cortisone, cortisol, corticosterone, 11-deoxycortisol, 11-deoxycorticosterone, androst...

journal_title：Clinical chemistry

authors： Wei JQ,Zhou XT,Wei JI

更新日期：1987-08-01 00:00:00

abstract：:Transcutaneous pCO2 electrode response time was optimized by use of a new electrode filling solution composed of NaCl/NaHCO3 electrolyte buffer (100 and 20 mmol/L, respectively) in an equivolume mixture of glycerol and water. The 95% response time to a step change in pCO2 was 49.9 +/- 2.8 s (mean +/- SD) when there wa...

journal_title：Clinical chemistry

authors： Kost GJ,Chow JL,Kenny M

更新日期：1982-07-01 00:00:00

abstract：:We detected increased concentrations of lipoperoxidation products, as malondialdehyde, in the serum of an infant with classic hemolytic-uremic syndrome. The concentrations declined when peritoneal dialysis was initiated and eventually returned to normal after clinical recovery. Our observation adds to the existing bod...

journal_title：Clinical chemistry

authors： Brown RE,Alade SL,Knight JA,Evans BJ

更新日期：1988-11-01 00:00:00

abstract：BACKGROUND:Heparinized plasma samples allow more rapid analysis than serum samples, but preliminary studies showed lower cardiac troponin T (cTnT) results in plasma. We undertook a multicenter study to characterize this effect for cTnT and cardiac troponin I (cTnI). METHODS:Blood samples were collected with and withou...

journal_title：Clinical chemistry

authors： Gerhardt W,Nordin G,Herbert AK,Burzell BL,Isaksson A,Gustavsson E,Haglund S,Müller-Bardorff M,Katus HA

更新日期：2000-06-01 00:00:00

abstract：:Phenobarbital and phenytoin are extracted and concentrated in a single step, without solvent evaporation, followed by on-column methylation and gas-chromatographic quanitation. A similar extraction step for the assay of theophylline by high-pressure liquid chromatography is described. The extraction step can be comple...

journal_title：Clinical chemistry

authors： Shihabi ZK

更新日期：1978-09-01 00:00:00

abstract：:We report here the evaluation of the Abbott TDx assay with a monoclonal antibody for selectively quantifying cyclosporine (CsA) in whole blood. Over the clinically relevant concentration ranges, results with this assay demonstrated within- and between-run CVs of less than 2.5% and 5%, respectively; sensitivity of 25 m...

journal_title：Clinical chemistry

authors： Yatscoff RW,Copeland KR,Faraci CJ

更新日期：1990-11-01 00:00:00

abstract：BACKGROUND:Adequate detection of designer steroids in the urine of athletes is still a challenge in doping control analysis and requires knowledge of steroid metabolism. In this study we investigated whether uPA(+/+)-SCID mice carrying functional primary human hepatocytes in their liver would provide a suitable alterna...

journal_title：Clinical chemistry

authors： Lootens L,Meuleman P,Pozo OJ,Van Eenoo P,Leroux-Roels G,Delbeke FT

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Cardiovascular mortality is markedly higher in Russia than in Western Europe and the US. Little is known about indicators of atherosclerotic risk in the Russian population. To our knowledge, this is the first study of apolipoprotein (apo) AI and B in Russia based on the WHO-IFCC standard. METHODS:We measure...

journal_title：Clinical chemistry

authors： Averina M,Nilssen O,Brenn T,Brox J,Arkhipovsky VL,Kalinin AG

更新日期：2004-02-01 00:00:00

abstract：BACKGROUND:Oxidation of lipids in lipoproteins and cells may initiate and enhance the early development of cardiovascular disease. METHOD AND RESULTS:We assayed F(2)-isoprostanes, oxidation products of arachidonic acid, by gas chromatography-mass spectrometry in a biracial cohort of 2850 young healthy adult men and wo...

journal_title：Clinical chemistry

authors： Gross M,Steffes M,Jacobs DR Jr,Yu X,Lewis L,Lewis CE,Loria CM

更新日期：2005-01-01 00:00:00

abstract：:Cerebrospinal fluid (CSF) from uninfected patients and from patients with bacterial and viral meningitis was analyzed by gas-liquid chromatography, with use of a flame ionization detector, and by gas chromatography-mass spectrometry. The resulting profiles were consistent and reproducible. Hydroxy acids were the compo...

journal_title：Clinical chemistry

authors： Perlman S,Carr SA

更新日期：1984-07-01 00:00:00

abstract：:Since 1991, the Ontario Laboratory Proficiency Testing Program has assessed the analytical performance of creatine kinase (CK; EC 2.7.3.2) isoenzyme-2, using fresh human serum supplemented with purified human CK isoenzymes. In Ontario, the 142 laboratories licensed to analyze CK-2 use a variety of methods: electrophor...

journal_title：Clinical chemistry

authors： Henderson AR,Stark JA,McQueen MJ,Patten RL,Krishnan S,Wood DE,Webb S

更新日期：1994-02-01 00:00:00

abstract：BACKGROUND:A simple point-of-care method for measuring leukocyte counts in a doctor's office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils ...

journal_title：Clinical chemistry

authors： Rundström G,Jonsson A,Mårtensson O,Mendel-Hartvig I,Venge P

更新日期：2007-02-01 00:00:00

abstract：:I describe a gas-chromatographic procedure involving a nitrogen detector for "free" (or "unbound") phenytoin in the concentration range 0.2-4.0 mg/L in ultrafiltrates of plasma. The CV (n = 10) for the assay was 2.9% for a 0.49 mg/L concentration, 1.5% for 2.02 mg/L. The sensitivity was 0.05 mg/L. The ultrafiltration ...

journal_title：Clinical chemistry

authors： Joern WA

更新日期：1981-03-01 00:00:00

abstract：BACKGROUND:Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnos...

journal_title：Clinical chemistry

authors： Kaleta EJ,Clark AE,Cherkaoui A,Wysocki VH,Ingram EL,Schrenzel J,Wolk DM

更新日期：2011-07-01 00:00:00

abstract：:We report results for adjusted ionized calcium (at pH 7.4) and actual ionized calcium (at actual pH) in capillary blood from 183 patients with disorders of calcium metabolism (primary hyperparathyroidism, secondary hyperparathyroidism of malabsorption, primary hypoparathyroidism, Paget's disease, acromegaly, hypercalc...

journal_title：Clinical chemistry

authors： Thode J,Holmegaard SN,Transbøl I,Fogh-Andersen N,Siggaard-Andersen O

更新日期：1990-03-01 00:00:00

abstract：BACKGROUND:Mass spectrometric assays could potentially replace protein immunoassays in many applications. Previous studies have demonstrated the utility of liquid chromatography-multiple-reaction monitoring-mass spectrometry (LC-MRM/MS) for the quantification of proteins in biological samples, and many examples of the ...

journal_title：Clinical chemistry

authors： Hoofnagle AN,Becker JO,Oda MN,Cavigiolio G,Mayer P,Vaisar T

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND:Blood concentrations of the calcineurin inhibitors (CNIs) cyclosporine and tacrolimus are currently measured to monitor immunosuppression in transplant patients. The measurement of calcineurin (CN) phosphatase activity has been proposed as a complementary pharmacodynamic approach. However, determining CN act...

journal_title：Clinical chemistry

authors： Carr L,Gagez AL,Essig M,Sauvage FL,Marquet P,Gastinel LN

更新日期：2014-02-01 00:00:00

abstract：:We examined the variability of lipid and lipoprotein concentrations in plasma from a population sample from the Jerusalem Lipid Research Clinic study. Coefficients of variation of about 8% for plasma cholesterol, 11% to 15% for low- and high-density-lipoprotein cholesterol, and about 30% for triglyceride were reported...

journal_title：Clinical chemistry

authors： Friedlander Y,Kark JD,Stein Y

更新日期：1985-07-01 00:00:00

abstract：:The IFCC-recommended procedure for aspartate aminotransferase (EC 2.6.1.1) was adapted to the centrifugal analyzer and evaluated during five years. The main hindrance to widespread use of the recommended method is the need for pre-incubation with pyridoxal phosphate. The present method includes a 10-min pre-incubation...

journal_title：Clinical chemistry

authors： Bruns DE,Savory J,Titheradge AC,Cross RE,Wills MR

更新日期：1981-01-01 00:00:00

abstract：:A novel noninstrumented technology (ChemTrak AccuMeter) for the quantitative measurement of analytes in biological fluids was reported at the 1990 Oak Ridge Conference (Clin Chem 1990;36:1591-7). This instrument-free technology has been adapted for the quantitative measurement of high-density lipoprotein (HDL). An in ...

journal_title：Clinical chemistry

authors： Liu VY,Lin TY,Schrier W,Allen M,Singh P

更新日期：1993-09-01 00:00:00

abstract：BACKGROUND:Reliable detection of neuroblastoma cells in bone marrow (BM) is critical because BM involvement influences staging, risk assessment, and evaluation of therapeutic response in neuroblastoma patients. Standard cytomorphologic examination of BM aspirates is sensitive enough to detect single tumor cells. Conseq...

journal_title：Clinical chemistry

authors： Swerts K,De Moerloose B,Dhooge C,Vandesompele J,Hoyoux C,Beiske K,Benoit Y,Laureys G,Philippé J

更新日期：2006-03-01 00:00:00

abstract：:We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight wit...

journal_title：Clinical chemistry

authors： Miyai K,Ishibashi K,Kawashima M

更新日期：1981-08-01 00:00:00

abstract：:We describe a "high-performance" liquid-chromatographic assay for measuring ritalinic acid in 300 muL of serum. The procedure includes adsorption of ritalinic acid and internal standard (alpha,alpha-dimethyl-beta-emthylsuccinimide) from serum onto charcoal, from which both compounds are eluted with methanol. The metha...

journal_title：Clinical chemistry

authors： Soldin SJ,Hill BM,Cahn YP,Swanson JM,Hill JG

更新日期：1979-01-01 00:00:00

abstract：BACKGROUND:Hemoglobin-based oxygen carriers (HBOCs) are being developed as potential substitutes for the oxygen-carrying functions of erythrocytes, but athletes may obtain and experiment with HBOCs as an illicit means of enhancing oxygen transport. An electrophoretic technique has been developed to screen for the prese...

journal_title：Clinical chemistry

authors： Varlet-Marie E,Ashenden M,Lasne F,Sicart MT,Marion B,de Ceaurriz J,Audran M

更新日期：2004-04-01 00:00:00

abstract：:The optimal measurement method and clinical application of the therapeutic drug monitoring of cyclosporine remain uncertain. At a workshop held at Hawk's Cay, FL, from January 14 to January 17, 1990, 57 scientists presented their latest research findings, either in formal papers or as discussants. Lively debate and di...

journal_title：Clinical chemistry

authors： Kahan BD,Shaw LM,Holt D,Grevel J,Johnston A

更新日期：1990-08-01 00:00:00

abstract：BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...

journal_title：Clinical chemistry

authors： Ho JE,Mahajan A,Chen MH,Larson MG,McCabe EL,Ghorbani A,Cheng S,Johnson AD,Lindgren CM,Kempf T,Lind L,Ingelsson E,Vasan RS,Januzzi J,Wollert KC,Morris AP,Wang TJ

更新日期：2012-11-01 00:00:00

abstract：:Using results from patients, we studied several methods for long-term (years) monitoring of the analytical stability of various chemistry tests. For each test, we obtained on a bimonthly basis histograms of > or = 200 to approximately 2000 data points and determined the median, mean, and mean of those data points in t...

journal_title：Clinical chemistry

authors： Lott JA,Smith DA,Mitchell LC,Moeschberger ML

更新日期：1996-06-01 00:00:00