Lateral flow immunoassay using Europium (III) chelate microparticles and time-resolved fluorescence for eosinophils and neutrophils in whole blood.

abstract：:A plasma protein required for the support of ristocetin-induced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 125I. A double-antibody radioimmunoassay was developed, with use of specific rabbit anti-VIII related antigen serum and goat anti-rabbit globulin. The assay is ...

journal_title：Clinical chemistry

authors： Green D,Reynolds N

更新日期：1977-09-01 00:00:00

abstract：:Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oligodeoxynucleotide detector probe hybridizes to the amplification prod...

journal_title：Clinical chemistry

authors： Walker GT,Nadeau JG,Linn CP,Devlin RF,Dandliker WB

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:We sought to develop a tandem mass spectrometry assay in which the enzymatic activities of 3 lysosomal enzymes (α-glucosidase, α-galactosidase A, and α-l-iduronidase) could be quantified in dried blood spots by using a single assay buffer. METHODS:A 3-mm dried blood spot punch was incubated in a single assa...

journal_title：Clinical chemistry

authors： Duffey TA,Bellamy G,Elliott S,Fox AC,Glass M,Turecek F,Gelb MH,Scott CR

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND:Insulin, growth hormone (GH), and growth factors (insulin-like growth factors [IGFs] and their binding proteins [IGFBPs]) are known to influence fetal growth and also the synthesis/secretion of the recently discovered hormones leptin and ghrelin. METHODS:In 153 delivering mothers and their offspring at birt...

journal_title：Clinical chemistry

authors： Chiesa C,Osborn JF,Haass C,Natale F,Spinelli M,Scapillati E,Spinelli A,Pacifico L

更新日期：2008-03-01 00:00:00

abstract：BACKGROUND:Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH). METHODS:After addition of sphinga...

journal_title：Clinical chemistry

authors： Groener JE,Poorthuis BJ,Kuiper S,Helmond MT,Hollak CE,Aerts JM

更新日期：2007-04-01 00:00:00

abstract：BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...

journal_title：Clinical chemistry

authors： Koziol JA,Ho NJ,Felitti VJ,Beutler E

更新日期：2001-10-01 00:00:00

abstract：:In this method for serum and urinary urea determination, the same reagent is used without predilution of urine samples. The method is based on the pH increase resulting from the ammonia released by urease hydrolysis of urea. o-Cresolphthalein complexone is used to monitor the pH change colorimetrically. Urea concentra...

journal_title：Clinical chemistry

authors： Orsonneau JL,Massoubre C,Cabanes M,Lustenberger P

更新日期：1992-05-01 00:00:00

abstract：:We recently proposed a quantitative PCR procedure for the absolute measurement of c-erbB-2 oncogene amplification, based on the simultaneous polymerase chain reaction (PCR) amplification of the target gene and of a competitor DNA molecule acting as internal standard. To increase the number of assayable oncogenes and t...

journal_title：Clinical chemistry

authors： Sestini R,Orlando C,Zentilin L,Lami D,Gelmini S,Pinzani P,Giacca M,Pazzagli M

更新日期：1995-06-01 00:00:00

abstract：:We evaluated six precipitation methods for high-density lipoprotein cholesterol (HDL-chol) determination: the heparin/Mn2+ precipitation reagent method (Hep), two variants of the phosphotungstic acid/Mg2+ method (Tung-L and Tung-B), the dextran sulfate 50,000/Mg2+ method (Dex), the PEG 6000 method (PEG), and the PEG 6...

journal_title：Clinical chemistry

authors： Demacker PN,Hessels M,Toenhake-Dijkstra H,Baadenhuijsen H

更新日期：1997-04-01 00:00:00

abstract：:In considering factors that might influence measurement of free thyroxin (T4), we evaluated the proportion (%) of T4 that could be stripped from thyroxin-binding globulin (TBG). The percentage of free T4 was measured in serially diluted sera from four normal subjects, four patients with hyperthyroidism or hypothyroidi...

journal_title：Clinical chemistry

authors： Nakagawa T,Matsumura K,Takeda K,Shinoda N,Matsuda A,Matsushita T,Tagami T

更新日期：1990-02-01 00:00:00

abstract：:We measured three renal tubular brush-border enzymes (lactate dehydrogenase, LDH, EC 1.1.1.27; gamma-glutamyltransferase, GGT, EC 2.3.2.2; and alkaline phosphatase, AP, EC 3.1.3.1) in morning urine samples from 48 healthy subjects to check whether different storage times and temperatures could modify enzyme concentrat...

journal_title：Clinical chemistry

authors： Matteucci E,Gregori G,Pellegrini L,Navalesi R,Giampietro O

更新日期：1991-08-01 00:00:00

abstract：BACKGROUND:Tamoxifen is a standard endocrine therapy for the prevention and treatment of steroid hormone receptor-positive breast cancer. CONTENT:Tamoxifen requires enzymatic activation by cytochrome P450 (CYP) enzymes for the formation of active metabolites 4-hydroxytamoxifen and endoxifen. As compared with the paren...

journal_title：Clinical chemistry

authors： Brauch H,Mürdter TE,Eichelbaum M,Schwab M

更新日期：2009-10-01 00:00:00

abstract：BACKGROUND:Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required. METHODS:We evaluated extracti...

journal_title：Clinical chemistry

authors： Bohmann K,Hennig G,Rogel U,Poremba C,Mueller BM,Fritz P,Stoerkel S,Schaefer KL

更新日期：2009-09-01 00:00:00

abstract：:We have observed an electrophoretically abnormal, nonfunctional species of alpha 1-antitrypsin in serum from patients who were receiving bone-marrow transplants for treatment of leukemia or aplastic anemia. Three of four patients in whose serum this protein appeared died soon after; the fourth recovered, and the disap...

journal_title：Clinical chemistry

authors： Mullins RE,Bennett B,Hunter RL Jr

更新日期：1987-01-01 00:00:00

abstract：:We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect i...

journal_title：Clinical chemistry

authors： Walmsley TA,Grant S,George PM

更新日期：1991-05-01 00:00:00

abstract：:Detection of intracellular antigens by flow cytometry requires effective fixation and permeabilization of the cell membrane. This study compares three fixation/permeabilization techniques: two commercial chemical reagents, the ORTHOPermeaFix (OPF) and the FIX&PERM Cell Permeabilization Kit (F&P), and a novel method ba...

journal_title：Clinical chemistry

authors： Millard I,Degrave E,Philippe M,Gala JL

更新日期：1998-11-01 00:00:00

abstract：BACKGROUND:The prognostic value of changes in paraprotein markers after stem cell transplantation is unknown. We evaluated disease response using serum immunofixation (s-IFIX), total kappa and lambda ratio (KLR), and free light chain (FLC) ratio in myeloma patients who underwent autologous or autologous plus allogeneic...

journal_title：Clinical chemistry

authors： Giarin MM,Giaccone L,Sorasio R,Sfiligoi C,Amoroso B,Cavallo F,Cipriani A,Palumbo A,Boccadoro M

更新日期：2009-08-01 00:00:00

abstract：:We describe the measurement of human immunoglobulins IgG, IgA, and IgM in diluted sera, with diluted commercial monospecific antisera, with use of a 36-place centrifugal analyzer (RotoChem IIa; American Instrument Co., Silver Spring, MD 20920). The assay involves a two-point kinetic turbidimetric technique. The change...

journal_title：Clinical chemistry

authors： Finley PR,Williams RJ,Lichti DA,Griffith F,Thies AC

更新日期：1979-04-01 00:00:00

abstract：:After extraction of the sample (on filter paper) in the counting vial, cortisol is assayed by adding 50 microL of horse serum containing tritiated cortisol, and 2 mL of toluene scintillator, shaking for 20 min, and counting the radioactivity in a liquid-scintillation counter at ambient temperature. The method can be a...

journal_title：Clinical chemistry

authors： Murphy BE,Barta A

更新日期：1987-07-01 00:00:00

abstract：:We describe a new liquid-chromatographic method with fluorescence detection for direct simultaneous determination of four urinary sulfate conjugates of 17-oxosteroids (17OS), without use of solvolysis or enzymatic hydrolysis. The 17OS sulfates in urine samples are extracted with benzene as ion pairs in the presence of...

journal_title：Clinical chemistry

authors： Nishikaze O,Iwata J

更新日期：1986-05-01 00:00:00

abstract：:We present a unified approach to the use of linear models and matrix least squares with the intention of providing a better understanding of the techniques themselves and of the statistics that arise from these techniques as they are used in clinical chemistry. Emphasis is placed on the importance of appropriate exper...

journal_title：Clinical chemistry

authors： Deming SN,Morgan SL

更新日期：1979-06-01 00:00:00

abstract：BACKGROUND:Identifying markers of chronic kidney disease (CKD) that occur early in the disease process and are specific to loss of kidney function rather than other underlying causes of disease may allow earlier, more accurate identification of patients who will develop CKD. We therefore sought to identify diagnostic b...

journal_title：Clinical chemistry

authors： Mohammadi-Shemirani P,Sjaarda J,Gerstein HC,Treleaven DJ,Walsh M,Mann JF,McQueen MJ,Hess S,Paré G

更新日期：2019-03-01 00:00:00

abstract：BACKGROUND:D-2-Hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-...

journal_title：Clinical chemistry

authors： Struys EA,Verhoeven NM,Roos B,Jakobs C

更新日期：2003-07-01 00:00:00

abstract：BACKGROUND:We investigated agreement between self-reported prenatal alcohol exposure (PAE) and objective meconium alcohol markers to determine the optimal meconium marker and threshold for identifying PAE. METHODS:Meconium fatty acid ethyl esters (FAEE), ethyl glucuronide (EtG), and ethyl sulfate (EtS) were quantified...

journal_title：Clinical chemistry

authors： Himes SK,Dukes KA,Tripp T,Petersen JM,Raffo C,Burd L,Odendaal H,Elliott AJ,Hereld D,Signore C,Willinger M,Huestis MA,Prenatal Alcohol in SIDS and Stillbirth (PASS) Network.

更新日期：2015-03-01 00:00:00

abstract：:Under the Clinical Laboratory Improvement Act of 1967 the Health Care Financing Administration's proficiency-testing requirement applies to approximately 12,000 hospital, reference, and large-clinic laboratories in the United States. The Wisconsin State Laboratory of Hygiene is approved by the Health Care Financing Ad...

journal_title：Clinical chemistry

authors： Lanphear BJ,Burmeister BJ,Ehrmeyer SS,Laessig RH,Hassemer DJ

更新日期：1992-07-01 00:00:00

abstract：:Proteinuria, long recognized as a sign of renal disease, is a common laboratory abnormality in children and adults. Proteinuria may be transient or persistent; when persistent, the cause must be determined. Proteinuria may be primarily glomerular or tubular in origin. Glomerular proteinuria is usually an important con...

journal_title：Clinical chemistry

authors： Waller KV,Ward KM,Mahan JD,Wismatt DK

更新日期：1989-05-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：:A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O-carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and a...

journal_title：Clinical chemistry

authors： Riad-Fahmy D,Read GF,Gaskell SJ,Dyas J,Hindawi R

更新日期：1979-05-01 00:00:00

abstract：BACKGROUND:Paternal zygosity testing is used for determining homo- or hemizygosity of RHD in pregnancies that are at a risk of hemolytic disease of the fetus and newborn. At present, this is achieved by using real-time PCR or the Rhesus box PCR, which can be difficult to interpret and unreliable, particularly for black...

journal_title：Clinical chemistry

authors： Sillence KA,Halawani AJ,Tounsi WA,Clarke KA,Kiernan M,Madgett TE,Avent ND

更新日期：2017-08-01 00:00:00

abstract：:Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for tra...

journal_title：Clinical chemistry

authors： Buffone GJ,Lewis SA,Iosefsohn M,Hicks JM

更新日期：1978-10-01 00:00:00