Abstract：
:We have observed an electrophoretically abnormal, nonfunctional species of alpha 1-antitrypsin in serum from patients who were receiving bone-marrow transplants for treatment of leukemia or aplastic anemia. Three of four patients in whose serum this protein appeared died soon after; the fourth recovered, and the disappearance of the abnormal alpha 1-antitrypsin paralleled his recovery. This suggests that the inability to maintain functional activity of alpha 1-antitrypsin predisposes patients to life-threatening complications during recovery from bone-marrow transplants.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Mullins RE,Bennett B,Hunter RL Jrsubject
Has Abstractpub_date
1987-01-01 00:00:00pages
193-5issue
1eissn
0009-9147issn
1530-8561journal_volume
33pub_type
杂志文章abstract：BACKGROUND:Dried blood spots (DBS) are a convenient and inexpensive method for biobanking. Although many countries have established population-based DBS biobanks from neonatal screening programs, the quality and usefulness of DNA from DBS have not been extensively assessed. METHODS:We compared 4 common DNA extraction ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.087510
更新日期：2007-08-01 00:00:00
abstract：:Zinc protoporphyrin is the predominent fluorescent porphyrin accumulating in erythrocytes as a result of chronic lead absorption or iron-deficiency anemia. Although diagnostic concentrations or thresholds for it in erythrocytes have been inferred, normal adult averages have not been established. We quantitatively asse...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：BACKGROUND:Detection of circulating tumor cells (CTCs) in the peripheral blood is a rapidly developing research field with clear clinical implications for the staging and monitoring of cancer patients. Current CTC assays, including the US Food and Drug Administration-cleared CellSearch® system, typically use markers [e...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2011.175570
更新日期：2012-05-01 00:00:00
abstract：:Comparison of two methods for determining a substance is a common procedure in clinical chemistry. Almost without exception, normal theory statistics are used with the tacit assumption that the data under test show gaussian distributions. Experience has led us to question this assumption for populations of differences...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactiv...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：BACKGROUND:To allow multianalyte binding assays, we have developed a novel polystyrene microtiter plate containing 24 main wells, each divided into 7 subwells. We explored its clinical potential by developing a PCR-chemiluminescent immunoassay (PCR-CLEIA) for simultaneous detection and typing of seven high oncogenic ri...
journal_title：Clinical chemistry
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：:We evaluated the HemoCue beta-Glucose Photometer system for use in our neonatal intensive care unit by assaying 178 heparinized whole-blood samples obtained by heel stick. The required sample size is 5 microL. Plasma glucose was analysed by a glucose oxidase/oxygen electrode methodology. Across the glucose range of 1....
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:We previously reported a family in which the propositus and both her father and paternal grandmother had high serum aminopeptidase N (CD13; EC 3.4.11.2) activity (autosomal dominant). The molecular mass of the serum CD13 polypeptide of the propositus was larger than that of normal CD13, suggesting either a m...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2001-02-01 00:00:00
abstract：:This ultrafiltration technique allows determination of free drug in 50 microL of serum. We ultrafiltered sera containing the following drugs--valproic acid (and its major metabolites), phenobarbital, diazepam, indomethacin, phenytoin, furosemide, and chloramphenicol--using both the commercially available micropartitio...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect i...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：BACKGROUND:Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required. METHODS:We evaluated extracti...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.122572
更新日期：2009-09-01 00:00:00
abstract：:Investigating the feasibility and validity of determining plasma ferritin concentration in blood obtained by finger prick, we studied 29 adults (ages 21-49 years) and 35 children (ages 14-66 months). Blood was sampled simultaneously in the same subject from both the antecubital vein (venous blood) and by finger pricki...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:We compared measurements of apoprotein A-I (apoA-I) in plasma and in heparin-MnCl2 supernates analyzed by radial immunodiffusion. The apoA-I values were similar when the samples were fresh [n = 41, mean (SD), mg/L: plasma, 1393.4(349); heparin-MnCl2 supernates, 1364.9(332), p less than 0.01], but were greater than 8% ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:Investigating the interference of danazol in testosterone radioimmunoassays, we measured serum testosterone concentrations in the serum of women receiving danazol, with three testosterone radioimmunoassay kits. All of these showed positive interference by danazol or its metabolites (or both). Values for testosterone o...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND:Human herpesvirus 6 (HHV-6) latently infects a majority of adults. In about 1% of the population HHV-6 exists in a chromosomally integrated form (ciHHV-6) that resides in every somatic and germ cell and can be transmitted through the germ line. Patients with ciHHV-6 have been misdiagnosed and unnecessarily t...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2013.217240
更新日期：2014-05-01 00:00:00
abstract：:We modified a one-step, two-site, double monoclonal immunoenzymometric assay (Abbott Laboratories) for serum alpha1-fetoprotein (AFP) to increase its sensitivity and improve test precision at the low end. We increased sample size, incubation interval, and reaction time and temperature, and decreased the final reaction...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-04-01 00:00:00
abstract：:This relatively simple, rapid method for quantification of homovanillic acid (HVA) and vanillylmandelic acid (VMA) involves solvent extraction and capillary gas chromatography. With use of this extraction method, the overall analytical recovery from an aqueous solution is 92.5% for HVA and 79.2% for VMA. 3,4-Dihydroxy...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-05-01 00:00:00
abstract：:A 56-year-old Samoan man with a documented history of hypertension, who presented with atrial fibrillation and hypotension, quickly developed renal failure with a serum urea nitrogen of 1.370 g/L and a serum creatinine of 92 mg/L. After hydration of the patient, the creatinine value became normal in two days, the urea...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:The isoenzyme pattern of N-acetyl-beta-glucosaminidase (NAG) in serum and urine was studied in two groups of patients with diabetes mellitus and in 30 control subjects. Total NAG activity was significantly (P less than 0.001) increased in the serum and urine of the 20 diabetics with vascular complications, but was ins...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-12-01 00:00:00
abstract：:The diagnosis of thyroid disease now often can be achieved reliably by measuring thyrotropin (TSH) alone. Thyroxine (T4), triiodothyronine, and other analytes are only needed if TSH and the accompanying clinical condition are discordant. We describe here work that confirms the age independence of TSH in both inpatient...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-11-01 00:00:00
abstract：:We evaluated a new discrete analyzer, the Hitachi 705. The instrument can process sequentially as many as 19 different enzymes or other analytes, with a throughput of 180 tests per hour. We evaluated the spectrophotometer, the sample and reagent probes, and the performance for 11 assays: creatine kinase, aspartate ami...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
abstract：BACKGROUND:Measurement of hemoglobin A1c (HbA1c) in the blood is integral to and essential for the treatment of patients with diabetes mellitus. HbA1c reflects the mean blood glucose concentration over the preceding 8 to 12 weeks. Although the clinical value of HbA1c was initially limited by large differences in result...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：10.1373/clinchem.2018.296962
更新日期：2019-07-01 00:00:00
abstract：:To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considerin...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight wit...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-08-01 00:00:00
abstract：:The Axial Separation Module (ASM), which separates whole-blood specimens serially in Axial Process Containers (APC), was evaluated for clinical performance at the University of Virginia Health Sciences Center (UVA HSC) in a community-based outpatient laboratory (North Ridge Clinic). We hypothesized that moving the tas...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:The tricyclic antidepressants amitriptyline, nortriptyline, imipramine, and desipramine in serum of patients taking one of the drugs were quantified in two laboratories by high-performance liquid chromatography (HPLC) and enzyme-multiplied immunoassay (EMIT; Syva). Results for split samples were highly correlated, but...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：BACKGROUND:B-type natriuretic peptide (BNP), which is activated in heart failure (HF), is processed to an active form by corin. The corin gene is expressed in the human heart and kidney, but corin protein expression in the heart, kidney, and circulation, along with whether proBNP is processed by circulating corin, rema...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2010.153908
更新日期：2011-01-01 00:00:00
abstract：:We studied the pattern of acute-phase proteins (orosomucoid, C-reactive protein, and haptoglobin) in hepatocellular deficiency due to chronic alcohol consumption, characterized by a decrease in serum transferrin concentration. We found that their patterns could vary independently of hepatocellular deficiency, but depe...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-01-01 00:00:00
abstract：:We directly compared the reliability of a single-isotope enzymatic derivative technique for measurement of plasma catecholamines with that of the well-established double-isotope method. A significant (p less than 0.001) correlation was observed between measurements (n = 52) in the two assays, both for norepinephrine (...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：BACKGROUND:Plasma concentrations of the peptides midregional proadrenomedullin (MR-proADM), midregional proatrial natriuretic peptide (MR-proANP), and C-terminal endothelin-1 precursor fragment (CT-proET-1) are increased in various cardiovascular conditions. However, there is limited information about the association a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2012.193656
更新日期：2013-04-01 00:00:00
