Abstract：
:We have studied the influence of triglyceride-rich particles on the analytical bias of apolipoprotein B measurements by various immunoturbidimetric methods. Three commercially available methods grossly overestimate apolipoprotein B in samples with even moderately above-normal triglyceride concentrations. This effect is due to the increased relative reactivity of very-low-density lipoproteins in these reagent systems, and can be eliminated by including Tween 20 (2 g/L) in the reagent buffer. We have developed, and describe, an automated immunoturbidimetric method that allows the accurate determination of apolipoprotein B in the plasma of patients with hypertriglyceridemia.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Walmsley TA,Grant S,George PMsubject
Has Abstractpub_date
1991-05-01 00:00:00pages
748-53issue
5eissn
0009-9147issn
1530-8561journal_volume
37pub_type
杂志文章abstract：:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-09-01 00:00:00
abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.132480
更新日期：2010-03-01 00:00:00
abstract：BACKGROUND:Serial measurements of neurohormones have been shown to improve prognostication in the setting of acute heart failure (HF) or chronic HF without therapeutic intervention. We investigated the prognostic role of serial measurements of emerging neurohormones and BNP in a cohort of chronic HF patients undergoing...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.125856
更新日期：2010-01-01 00:00:00
abstract：:A rapid technique (5-10 min) has been developed for fractionating nucleotides from base and nucleoside contaminants in acid extracts of cells, by adsorption to silica gels. Silica gels (1-mL bed volume) were washed with 5 mL of water then with 5 mL of acetonitrile/water (90/10 by vol). After applying 3-mL samples, adj...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-09-01 00:00:00
abstract：:Numerous xenobiotics regulate cellular functions by altering transcription of target genes. Use of sensitive and specific biomarkers based on gene transcript levels may help clarify the shape of the dose-response curve in the low-dose region associated with human exposures to environmental concentrations of chemicals....
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1995-12-01 00:00:00
abstract：:The reaction of 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB, Ellman's reagent) with thiols is sensitive to daylight, in particular to ultraviolet radiation at wavelengths around 325 nm. Exposure to light at the absorbance maximum of the yellow product (the thionitrobenzoate ion) at 410 nm had no effect on the reaction. ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：BACKGROUND:Disease-defining chromosomal translocations are seen in various neoplasms, especially in lymphomas and leukemias. Translocation detection at the DNA level is often complicated by chromosomal breakpoints that are distributed over very large regions. We have developed a ligation-based assay [the looped ligatio...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2016.270140
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2014.226571
更新日期：2015-01-01 00:00:00
abstract：:We describe a gas--liquid-chromatographic method for determining the concentration of amantadine hydrochloride in urine with beta-phenylethylamine as internal standard. The urine sample is made alkaline and extracted with 0.5 mL of chloroform. After centrifugation the aqueous layer is aspirated, and an aliquot of the ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-02-01 00:00:00
abstract：:We report the presence of complexes between aspartate aminotransferase (AST, EC 2.6.1.1) and immunoglobulin (Ig) in the serum of a patient suffering from lung cancer with metastasis to the liver. After fractionation of the serum by gel filtration, AST-Ig complexes (AST-IgA, AST-IgG) were demonstrated by counterimmunoe...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-02-01 00:00:00
abstract：BACKGROUND:Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable met...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2015.244004
更新日期：2016-01-01 00:00:00
abstract：:Highly purified human lactate dehydrogenases I and V were assayed in 17 different buffers, at a variety of reaction pH's. Diethanolamine and 2-amino-2-methyl-1,3-propanediol provided the best measurements of the enzyme, assayed lactate-to-pyruvate. However, the commercial preparation of 2-amino-2-methyl-1,3-propanedio...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
abstract：:We conducted a proficiency survey of cholesterol and high-density lipoprotein (HDL) cholesterol analysis in local clinical laboratories to determine whether increased national emphasis on cholesterol measurement had resulted in changes in performance from previous surveys. Sets of frozen aliquots of plasma and HDL sup...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:We describe a novel assay for measuring glycohemoglobin directly from anticoagulated whole blood with the Abbott IMx analyzer. The glycohemoglobin is labeled with a soluble polyanionic affinity reagent and the anionic complex is then captured with a cationic solid-phase matrix. Glycohemoglobin is quantified by measuri...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:We describe an enzymatic fluorometric method for determining glucose concentrations in blood samples by analysis on a semi-solid surface (silicone-rubber pads). The method is an adaptation of existing spectrophotometric procedures based on the sequential coupling reactions with glucose-6-phosphate dehydrogenase and NA...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1975-11-01 00:00:00
abstract：BACKGROUND:Associations between dairy intake and body composition and cardiometabolic traits have been inconsistently observed in epidemiological studies, and the causal relationship remains ill-defined. METHODS:We performed Mendelian randomization analysis using an established genetic variant located upstream of the ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2018.300335
更新日期：2019-06-01 00:00:00
abstract：:A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out an...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：BACKGROUND:Immunoassays specific for 1-84 parathyroid hormone (PTH) reportedly reflect the bioactivity of PTH; however, PTH immunoassays can be susceptible to interference by cross-reacting PTH fragments. In addition, these assays currently lack standardization. A methodology using immunocapture purification with liqui...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.134643
更新日期：2010-02-01 00:00:00
abstract：BACKGROUND:We analyzed the low-dose (1 microg) rapid adrenocorticotropic hormone test (LDST) in 17 patients with a normal hypothalamic-pituitary-adrenal axis to determine reference intervals for the LDST on the basis of poststimulation cortisol increments. METHODS:We analyzed test results for 17 patients (14 females a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.061267
更新日期：2006-04-01 00:00:00
abstract：:We evaluated a method for quantifying high-density lipoprotein cholesterol in plasma, based on electrophoretic migration of the prestained (with Sudan Black III) sample through a discontinuous polyacrylamide++ gel and densitometric integration of the stain associated with each class of lipoprotein. With this method, o...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:Comparison of two methods for determining a substance is a common procedure in clinical chemistry. Almost without exception, normal theory statistics are used with the tacit assumption that the data under test show gaussian distributions. Experience has led us to question this assumption for populations of differences...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:In this rapid, cost-effective, enzyme-linked immunosorbent assay for 17 alpha-hydroxyprogesterone (17-OHP) eluted from dried blood spotted on filter paper, second antibody is coated onto the microwell plate and horseradish peroxidase (EC 1.11.1.7) is the label enzyme. Antiserum to 17-OHP was prepared by using 4-(2-car...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-06-01 00:00:00
abstract：BACKGROUND:Cross-sectional studies have reported seasonal variation in high-sensitivity C-reactive protein (hsCRP). However, longitudinal data are lacking. METHODS:We collected data on diet, physical activity, psychosocial factors, physiology, and anthropometric measurements from 534 healthy adults (mean age 48 years,...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.111245
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:The management of critically ill infants and neonates includes frequent determination of arterial blood gas, electrolyte, and hematocrit values. An objective of attached point-of-care patient monitoring is to provide clinically relevant data without the adverse consequences associated with serial phlebotomy....
journal_title：Clinical chemistry
pub_type： 杂志文章,多中心研究
doi：
更新日期：2002-11-01 00:00:00
abstract：:To evaluate their potential usefulness in the differential diagnosis of Cushing's syndrome, we estimated the urinary excretion rates of the following non-metabolized, unbound steroid hormones: pregnenolone, progesterone, 17-OH-pregnenolone, 17-OH-progesterone, dehydroepiandrosterone (DHEA), androstenedione, testostero...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND:In recent years, there have been significant advances in the development of enzyme replacement and other therapies for lysosomal storage disorders (LSDs). Early diagnosis, before the onset of irreversible pathology, has been demonstrated to be critical for maximum efficacy of current and proposed therapies. ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:In this simple and low-cost radioimmunoassay (RIA) procedure for thyroxin (T4), ethacridine (6,9-diamino-2-ethoxyacridine lactate; Rivanol) is used to separate antibody-bound ligand and free ligand. This acridine dye precipitates free T4 not bound to antibody. At pH 8.6, it precipitates more than 90% of 125I-labeled T...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2004.046995
更新日期：2005-08-01 00:00:00
abstract：:The serum pancreolauryl test has limited sensitivity for detecting mild pancreatic insufficiency. The aim of this study was to optimize the serum pancreolauryl test so as to increase the probability of positive results in patients with chronic pancreatitis. The study had three parts. First, the sampling time was optim...
journal_title：Clinical chemistry
pub_type： 临床试验,杂志文章
doi：
更新日期：1998-04-01 00:00:00
abstract：:Serum amyloid A (SAA), a sensitive acute-phase protein, is the precursor of AA fibrils in reactive amyloidosis. However, SAA is poorly immunogenic, and development and standardization of immunoassays of this protein have been difficult. We established an automated polyclonal/monoclonal microparticle capture enzyme imm...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1994-07-01 00:00:00
