Fluorometric determination of erythrocyte protoporphyrin in blood, a comparison between direct (hematofluorometric) and indirect (extraction) methods.

Abstract:

:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct determination of protoporphyrin in the same samples. In the direct assay the fluorescence of protoporphyrin, present in blood as the zinc salt, was measured without extraction, with the use of a hematofluorometer. Comparison of the results of the direct and indirect methods showed an excellent correlation (r = 0.986), but the hematofluorometric values (y) were approximately 9% lower than the fluorometric values (y = 0.911x). The slope of the regression line (0.911) is likely to be different for each hematofluorometer-fluorometer combination. Therefore, hematofluorometric values can only be compared with fluorometric values if the slope of the regression line is known and is used as a correction factor.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Peter F,Growcock G,Strunc G

subject

Has Abstract

pub_date

1978-09-01 00:00:00

pages

1515-7

issue

9

eissn

0009-9147

issn

1530-8561

journal_volume

24

pub_type

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