Enzymatic determination of anti-NAD+ glycohydrolase in serum.

Abstract:

:A colorimetric method for measuring anti-NAD+ glycohydrolase in human sera has been developed. The assay involves the inhibition of NAD+ glycohydrolase (EC 3.2.2.5) by the antibody and determination of the noninhibited enzyme activity by using an enzymatic amplifying system for NAD+. The assay is easily carried out and has the additional advantage of a direct relationship between signal and antibody concentration. The results obtained for 100 human sera compare favorably with other tests commonly used to obtain evidence of streptococcal infections or their complications, such as the anti-streptolysin O and the anti-DNase B tests.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Gella FJ,Viver J,Canalias F,Beleta J,Gonzalez-Sastre F

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

1007-11

issue

6

eissn

0009-9147

issn

1530-8561

journal_volume

39

pub_type

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