Strand displacement amplification (SDA) and transient-state fluorescence polarization detection of Mycobacterium tuberculosis DNA.

abstract：BACKGROUND:Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in i...

journal_title：Clinical chemistry

authors： Campbell DJ,Nussberger J,Stowasser M,Danser AH,Morganti A,Frandsen E,Ménard J

更新日期：2009-05-01 00:00:00

abstract：:Nitric oxide is a soluble gas continuously synthesized by the endothelium. This substance has a wide range of biological properties that maintain vascular homeostasis, including modulation of vascular dilator tone, regulation of local cell growth, and protection of the vessel from injurious consequences of platelets a...

journal_title：Clinical chemistry

authors： Cannon RO 3rd

更新日期：1998-08-01 00:00:00

abstract：:We describe a ratio gas-chromatographic method for determination of cortisol production rate by measuring the isotope dilution of urinary cortisol metabolites. The method was calibrated by analyzing [3H]tetrahydrocortisol and [3H]tetrahydrocortisone of known specific activities. Results are reasonably well reproducibl...

journal_title：Clinical chemistry

authors： Derks HJ,Muskiet FA,Wolthers BG,Thijssen JH,Drayer NM

更新日期：1977-03-01 00:00:00

abstract：:The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the cal...

journal_title：Clinical chemistry

authors： Kemp GJ

更新日期：1984-07-01 00:00:00

abstract：:We measured serum protein and calcium concentrations in 2340 individuals between 10 and 96 years of age from 900 families chosen by probability methods to give a representative population. These values were used to calculate an index, based on a regression analysis of serum protein on calcium, which was then treated a...

journal_title：Clinical chemistry

authors： Kelly A,Munan L,PetitClerc C,Ho KP,Billon B

更新日期：1976-10-01 00:00:00

abstract：:Recombinant DNA techniques are contributing to the understanding of the pathogeneses of genetic, neoplastic, and viral diseases, and are used in the diagnosis of certain genetic and viral diseases. Such techniques will have wider application in the future and will play an increasing role in the clinical laboratory. Th...

journal_title：Clinical chemistry

authors： Sauls CD,Caskey CT

更新日期：1985-06-01 00:00:00

abstract：BACKGROUND:Detection of melanoma cells in circulation may be important in assessing tumor progression. The objective of this study was to develop a specific, reliable multimarker quantitative real-time reverse transcription-PCR (qRT) assay for detecting melanoma cells in patients' blood. METHODS:We developed qRT assay...

journal_title：Clinical chemistry

authors： Koyanagi K,Kuo C,Nakagawa T,Mori T,Ueno H,Lorico AR Jr,Wang HJ,Hseuh E,O'Day SJ,Hoon DS

更新日期：2005-06-01 00:00:00

abstract：:Alanine aminotransferase activity in serum increases significantly when serum is incubated with pyridoxal phosphate. The increase depends on the L-alanine concentration in the final assay mixture, being greatest at 800 mmol/liter. Preincubation of 22 normal sera, in a 10:1 ratio with an 8.09 mmol/liter pyridoxal phosp...

journal_title：Clinical chemistry

authors： Lustig V

更新日期：1977-02-01 00:00:00

abstract：BACKGROUND:Protein tyrosine phosphatase 1B (PTPN1) dephosphorylates insulin receptors and attenuates insulin signaling. Polymorphisms in the coding sequence of PTPN1 have been variably associated with type 2 diabetes (T2D). We hypothesized that variations within the PTPN1 promoter might contribute to the development of...

journal_title：Clinical chemistry

authors： Meshkani R,Taghikhani M,Al-Kateb H,Larijani B,Khatami S,Sidiropoulos GK,Hegele RA,Adeli K

更新日期：2007-09-01 00:00:00

abstract：BACKGROUND:External quality assurance (EQA) programs for diagnostic tests based on nucleic acid amplification have not been widely implemented in clinical laboratories and remain limited to few tests. Development of specific EQA programs based on application-based proficiency testing for any diagnostic molecular target...

journal_title：Clinical chemistry

authors： Raggi CC,Pinzani P,Paradiso A,Pazzagli M,Orlando C

更新日期：2003-05-01 00:00:00

abstract：BACKGROUND:Cardiac troponins are associated with cardiovascular risk in the general population, but whether temporal changes in cardiac troponin I provide independent prognostic information remains uncertain. Using a large community-based cohort with follow-up close to the present day, we aimed to investigate the assoc...

journal_title：Clinical chemistry

authors： Lyngbakken MN,Røsjø H,Holmen OL,Dalen H,Hveem K,Omland T

更新日期：2019-07-01 00:00:00

abstract：:We compared results for measurements of creatine kinase isoenzyme MB (CK-MB) by immunoinhibition vs immunoprecipitation, using sera from 53 normal healthy individuals, 55 patients with increased CK-MB associated with acute myocardial infarction, and 42 patients whose blood exhibited one or more abnormal forms of CK by...

journal_title：Clinical chemistry

authors： Wu AH,Bowers GN Jr

更新日期：1982-10-01 00:00:00

abstract：:We review the following labels used as substitutes for radioisotopes in immunoassay systems: bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performi...

journal_title：Clinical chemistry

authors： Schall RF Jr,Tenoso HJ

更新日期：1981-07-01 00:00:00

abstract：BACKGROUND:Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnos...

journal_title：Clinical chemistry

authors： Kaleta EJ,Clark AE,Cherkaoui A,Wysocki VH,Ingram EL,Schrenzel J,Wolk DM

更新日期：2011-07-01 00:00:00

abstract：:We have designed a re-usable dialysis cell and a complex dialysis buffer, with which undiluted serum samples can be dialyzed with minimal changes in their serum matrix. Dialysate thyroxin (free T4) is then measured by a sensitive RIA for T4. The range of reportability was 2-128 ng/L, the normal range was 8-27 ng/L, an...

journal_title：Clinical chemistry

authors： Nelson JC,Tomei RT

更新日期：1988-09-01 00:00:00

abstract：:We present a unified approach to the use of linear models and matrix least squares with the intention of providing a better understanding of the techniques themselves and of the statistics that arise from these techniques as they are used in clinical chemistry. Emphasis is placed on the importance of appropriate exper...

journal_title：Clinical chemistry

authors： Deming SN,Morgan SL

更新日期：1979-06-01 00:00:00

abstract：BACKGROUND:The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO(2) particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). ME...

journal_title：Clinical chemistry

authors： Wang H,Zhang Y,Yan B,Liu L,Wang S,Shen G,Yu R

更新日期：2006-11-01 00:00:00

abstract：BACKGROUND:Non-(1-84) parathyroid hormone (PTH) fragments are large C-terminal fragments of PTH with a partially preserved N-terminal structure. They differ from other C-terminal PTH fragments, which do not have an N-terminal structure and do not react in intact PTH assays. We aimed to identify the minimal N-terminal s...

journal_title：Clinical chemistry

authors： D'Amour P,Brossard JH,Räkel A,Rousseau L,Albert C,Cantor T

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:A sensitive gas chromatography-negative ion chemical ionization mass spectrometry (GC/MS-NICI) method was developed and validated for the measurement of Delta(9)-tetrahydrocannabinol (THC) in human sweat patches. METHODS:THC-d(0) and THC-d(3) were added to worn blank sweat patches (PharmChek; PharmChem Inco...

journal_title：Clinical chemistry

authors： Saito T,Wtsadik A,Scheidweiler KB,Fortner N,Takeichi S,Huestis MA

更新日期：2004-11-01 00:00:00

abstract：BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation ac...

journal_title：Clinical chemistry

authors： Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret P

更新日期：2007-10-01 00:00:00

abstract：:We investigated the effects on plasma osteocalcin concentrations of different anticoagulants used to collect the blood samples. Plasma osteocalcin concentrations measured by enzyme immunoassay and radioimmunoassay are influenced by the nature of the anticoagulants used. The most significant difference between concentr...

journal_title：Clinical chemistry

authors： Power MJ,O'Dwyer B,Breen E,Fottrell PF

更新日期：1991-02-01 00:00:00

abstract：:The precision of estimates of the sensitivity of diagnostic tests is evaluated. "Sensitivity" is defined as the fraction of diseased subjects with test values exceeding the 0.975-fractile of the distribution of control values. An estimate of the sensitivity is subject to sample variation because of variation of both c...

journal_title：Clinical chemistry

authors： Linnet K

更新日期：1985-04-01 00:00:00

abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...

journal_title：Clinical chemistry

authors： Gellekink H,van Oppenraaij-Emmerzaal D,van Rooij A,Struys EA,den Heijer M,Blom HJ

更新日期：2005-08-01 00:00:00

abstract：:We report an easy, rapid method for quantifying bone isoenzyme of alkaline phosphatase (EC 3.1.3.1., ALP) in serum. The original method described by Rosalki and Ying Foo (Clin Chem 1984;30:1182-6) was somewhat simplified. In contrast to their results, we found that bone ALP is precipitated quantitatively by wheat-germ...

journal_title：Clinical chemistry

authors： Behr W,Barnert J

更新日期：1986-10-01 00:00:00

abstract：BACKGROUND:A unique requirement for the molecular diagnosis of mitochondrial DNA (mtDNA) disorders is the ability to detect heteroplasmic mtDNA mutations and to distinguish them from homoplasmic sequence variations before further testing (e.g., sequencing) is performed. We evaluated the potential utility of temporal te...

journal_title：Clinical chemistry

authors： Chen TJ,Boles RG,Wong LJ

更新日期：1999-08-01 00:00:00

abstract：BACKGROUND:PS-341, a selective inhibitor of the proteasome, currently is under evaluation as an anticancer agent in multiple phase I clinical trials. In animal-model studies, PS-341 was rapidly removed from the vascular compartment and distributed widely, quickly approaching the limits of detection. An accurate pharmac...

journal_title：Clinical chemistry

authors： Lightcap ES,McCormack TA,Pien CS,Chau V,Adams J,Elliott PJ

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular tool in many diagnostic fields represents an affordable (approxim...

journal_title：Clinical chemistry

authors： Daher RK,Stewart G,Boissinot M,Bergeron MG

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:Little is known about lipoprotein(a) [Lp(a)] as a predictor of vascular events among ethnic Chinese. We prospectively investigated the association of Lp(a) with cardiovascular disease and all-cause death in a community-based cohort. METHODS:We conducted a community-based prospective cohort study of 3484 par...

journal_title：Clinical chemistry

authors： Chien KL,Hsu HC,Su TC,Sung FC,Chen MF,Lee YT

更新日期：2008-02-01 00:00:00

abstract：BACKGROUND:Rapid intraoperative parathyroid hormone (PTH) measurements are an important prerequisite for minimally invasive parathyroidectomy, serving as a feasible marker for "cure" because of the short half-life of PTH. Because automated analysis may facilitate monitoring, two automated PTH assays were compared with ...

journal_title：Clinical chemistry

authors： Bieglmayer C,Prager G,Niederle B

更新日期：2002-10-01 00:00:00

abstract：BACKGROUND:An association has been described between mortality in children with meningococcal disease and functional polymorphisms in the interleukin-1 (IL1) cluster. We undertook a multicenter study to evaluate associations of these polymorphisms in a Central European population. PATIENTS AND METHODS:The study involv...

journal_title：Clinical chemistry

authors： Endler G,Marculescu R,Starkl P,Binder A,Geishofer G,Müller M,Zöhrer B,Resch B,Zenz W,Mannhalter C,Central European Meningococcal Genetic Study Group.

更新日期：2006-03-01 00:00:00