Abstract：
BACKGROUND:Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in information provided by activity assays and immunoassays for renin and prorenin measurement and has drawn attention to the need for precautions to ensure their accurate measurement. CONTENT:Renin activity assays and immunoassays provide related but different information. Whereas activity assays measure only active renin, immunoassays measure both active and inhibited renin. Particular care must be taken in the collection and processing of blood samples and in the performance of these assays to avoid errors in renin measurement. Both activity assays and immunoassays are susceptible to renin overestimation due to prorenin activation. In addition, activity assays performed with peptidase inhibitors may overestimate the degree of inhibition of PRA by renin inhibitor therapy. Moreover, immunoassays may overestimate the reactive increase in plasma renin concentration in response to renin inhibitor therapy, owing to the inhibitor promoting conversion of prorenin to an open conformation that is recognized by renin immunoassays. CONCLUSIONS:The successful application of renin assays to patient care requires that the clinician and the clinical chemist understand the information provided by these assays and of the precautions necessary to ensure their accuracy.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Campbell DJ,Nussberger J,Stowasser M,Danser AH,Morganti A,Frandsen E,Ménard Jdoi
10.1373/clinchem.2008.118000subject
Has Abstractpub_date
2009-05-01 00:00:00pages
867-77issue
5eissn
0009-9147issn
1530-8561pii
clinchem.2008.118000journal_volume
55pub_type
杂志文章,评审abstract：:A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without...
journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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pub_type： 杂志文章
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journal_title：Clinical chemistry
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