Polymorphisms within the protein tyrosine phosphatase 1B (PTPN1) gene promoter: functional characterization and association with type 2 diabetes and related metabolic traits.

abstract：:Solid-phase-based immunoassays have traditionally been plagued by nonspecific binding to the solid phase and by slow reaction kinetics relative to reactants that are free to diffuse in solution. We have developed two novel immunoassays in which the solid phase is generated in situ after the specific binding reaction h...

journal_title：Clinical chemistry

authors： Auditore-Hargreaves K,Houghton RL,Monji N,Priest JH,Hoffman AS,Nowinski RC

更新日期：1987-09-01 00:00:00

abstract：:The optimal measurement method and clinical application of the therapeutic drug monitoring of cyclosporine remain uncertain. At a workshop held at Hawk's Cay, FL, from January 14 to January 17, 1990, 57 scientists presented their latest research findings, either in formal papers or as discussants. Lively debate and di...

journal_title：Clinical chemistry

authors： Kahan BD,Shaw LM,Holt D,Grevel J,Johnston A

更新日期：1990-08-01 00:00:00

abstract：:We compared the analytical performance of the Kodak Ektachem XR700 assays of iron (Fe) and total iron-binding capacity (TIBC) with that of a conventional ferrozine assay (performed with a Cobas-Bio) and occasionally with that of atomic-absorption spectrometry (AAS). The correlation was modest between Kodak and Cobas-B...

journal_title：Clinical chemistry

authors： Vercammen M,Goedhuys W,Boeyckens A,De Roy R,Sennesael J,Sevens C,Gorus F

更新日期：1990-10-01 00:00:00

abstract：BACKGROUND:Iron may promote coronary atherosclerotic disease (CAD) by increasing lipid peroxidation. Studies on biochemical or genetic markers of body iron stores as risk factors for CAD have yielded conflicting results. METHODS:We studied 849 individuals with a clear-cut definition of the CAD phenotype, i.e., with (C...

journal_title：Clinical chemistry

authors： Bozzini C,Girelli D,Tinazzi E,Olivieri O,Stranieri C,Bassi A,Trabetti E,Faccini G,Pignatti PF,Corrocher R

更新日期：2002-01-01 00:00:00

abstract：:We compared cardiac troponin I (cTnI), using Access, Sanofi Pasteur, and cardiac troponin T (cTnT), using Elecsys, Boehringer Mannheim, in the first two routine blood samplings in a routine panel of cardiac markers for the biochemical diagnostic evaluation of patients with symptoms of acute myocardial infarction (AMI)...

journal_title：Clinical chemistry

authors： Hetland O,Dickstein K

更新日期：1998-07-01 00:00:00

abstract：BACKGROUND:Previous methods to measure glycohemoglobin (GHb) have been time-consuming or imprecise; we therefore developed a new enzymatic assay for GHb. METHODS:Blood cells were first hemolyzed, and hemoglobin was digested with protease to yield fructosyl amino acid. Fructosyl amino acid oxidase acts on the fructosyl...

journal_title：Clinical chemistry

authors： Sakurabayashi I,Watano T,Yonehara S,Ishimaru K,Hirai K,Komori T,Yagi M

更新日期：2003-02-01 00:00:00

abstract：BACKGROUND:Kallikrein-related peptidase 6 (KLK6), a member of the serine protease family of kallikrein (KLK) genes, is dysregulated in ovarian carcinomas (OCa) and its overexpression is associated with poor prognosis. Regulation of its expression is poorly understood and is likely to be influenced by multiple mechanism...

journal_title：Clinical chemistry

authors： Bayani J,Kuzmanov U,Saraon P,Fung WA,Soosaipillai A,Squire JA,Diamandis EP

更新日期：2013-01-01 00:00:00

abstract：:The topic of quality control often evokes prolific discussion among those whose business is its administration. Reliable methods for identifying mistakes must be developed in both the manufacture and use of test devices. Unit test devices are uncomplicated to run and provide quick results in a world where speed and si...

journal_title：Clinical chemistry

authors： Blase AB

更新日期：1997-05-01 00:00:00

abstract：BACKGROUND:Δ(9)-Tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), and cannabinol (CBN) were measured in breath following controlled cannabis smoking to characterize the time course and windows of detection of breath cannabinoids. METHODS:Exhaled breath was collected from chronic (≥4 times per week) and occas...

journal_title：Clinical chemistry

authors： Himes SK,Scheidweiler KB,Beck O,Gorelick DA,Desrosiers NA,Huestis MA

更新日期：2013-12-01 00:00:00

abstract：:We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly sim...

journal_title：Clinical chemistry

authors： Magnotti RA Jr,Stephens GW,Rogers RK,Pesce AJ

更新日期：1989-07-01 00:00:00

abstract：:Detection and characterization of small IgM paraproteins by immunoelectrophoresis are often difficult because of (a) slow diffusion of the macromolecular immunoglobulin, and (b) the obscuring of the light chain, especially kappa, by the light chains of background polyclonal immunoglobulin (the "umbrella effect"). Vari...

journal_title：Clinical chemistry

authors： Kahn SN,Bina M

更新日期：1988-08-01 00:00:00

abstract：:We have used a monoclonal antibody-based ELISA for plasma prothrombin fragment 1.2 (F1.2) to establish appropriate sample collection and storage conditions for this biomarker of thrombin generation. F1.2 concentrations were not altered by exogenous factor Xa, thrombin, or thromboplastin if blood was collected by routi...

journal_title：Clinical chemistry

authors： Greenberg CS,Hursting MJ,Macik BG,Ortel TL,Kane WH,Moore BM

更新日期：1994-10-01 00:00:00

abstract：BACKGROUND:Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting ana...

journal_title：Clinical chemistry

authors： Seipp MT,Pattison D,Durtschi JD,Jama M,Voelkerding KV,Wittwer CT

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:In recent years, there have been significant advances in the development of enzyme replacement and other therapies for lysosomal storage disorders (LSDs). Early diagnosis, before the onset of irreversible pathology, has been demonstrated to be critical for maximum efficacy of current and proposed therapies. ...

journal_title：Clinical chemistry

authors： Umapathysivam K,Whittle AM,Ranieri E,Bindloss C,Ravenscroft EM,van Diggelen OP,Hopwood JJ,Meikle PJ

更新日期：2000-09-01 00:00:00

abstract：:gamma-Aminobutyric acid (GABA), an inhibitory neurotransmitter that serves about one-third of brain neurons, is involved in the development of depression and in the treatment of depression and mania with pharmacological therapy. Brain activity of GABA may be conveniently measured in plasma, and changes in plasma conce...

journal_title：Clinical chemistry

authors： Petty F

更新日期：1994-02-01 00:00:00

abstract：:We compared measurements of apoprotein A-I (apoA-I) in plasma and in heparin-MnCl2 supernates analyzed by radial immunodiffusion. The apoA-I values were similar when the samples were fresh [n = 41, mean (SD), mg/L: plasma, 1393.4(349); heparin-MnCl2 supernates, 1364.9(332), p less than 0.01], but were greater than 8% ...

journal_title：Clinical chemistry

authors： Jiang XR,Bachorik PS

更新日期：1986-06-01 00:00:00

abstract：:Carbohydrate-deficient transferrin (CDT) may now be the most valuable biological marker for diagnosis of alcohol abuse. We compared the diagnostic performance of two new CDT tests, Axis %CDT turbidimetric immunoassay (TIA) and Axis %CDT HPLC, against Specialty Laboratories' isoelectric focusing/immunoblotting/laser de...

journal_title：Clinical chemistry

authors： Bean P,Liegmann K,Løvli T,Westby C,Sundrehagen E

更新日期：1997-06-01 00:00:00

abstract：BACKGROUND:Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape and characteristics of extracellular vesicle long RNA (exLR) in human blood remain largely unknown. METHODS:We presented an optimized strategy for exLR sequ...

journal_title：Clinical chemistry

authors： Li Y,Zhao J,Yu S,Wang Z,He X,Su Y,Guo T,Sheng H,Chen J,Zheng Q,Li Y,Guo W,Cai X,Shi G,Wu J,Wang L,Wang P,He X,Huang S

更新日期：2019-06-01 00:00:00

abstract：:We compared results obtained with the Beckman Creatinine Analyzer (Beckman Instruments, Inc.) to those with the GEMSAEC Centrifugal Analyzer (Electro-Nucleonics, Inc.) and with a manual method for determination of creatinine in serum and urine. Linearity, stability, recovery, and precision were evaluated. Interference...

journal_title：Clinical chemistry

authors： Hicks JM,Iosefsohn M,Lewis SA

更新日期：1979-06-01 00:00:00

abstract：:Sensitivity, stability, and specificity of the color-producing reaction of hydrogen peroxide with bezidine, leuco-malachite green, or o-dianisidine were tested in numerous systems containing hemoglobin and other hemoproteins. Use of urea or low temperature (to minus 12 degrees C), or both, was highly beneficial, espec...

journal_title：Clinical chemistry

authors： Ahlquist DA,Schwartz S

更新日期：1975-03-01 00:00:00

abstract：:We report a simple, enzymatic, end point method for determining nitrate in serum and urine with use of nitrate reductase from Aspergillus sp. (EC 1.6.6.2). The decrease in absorbance at 340 nm as a result of the oxidation of beta-NADPH is measured. We used FAD as a supplementary electron carrier and added an internal ...

journal_title：Clinical chemistry

authors： Bories PN,Bories C

更新日期：1995-06-01 00:00:00

abstract：:Measurement of alpha-fetoprotein concentration and acetylcholinesterase activity in amniotic fluid can be used to identify chromosomal defects as well as neural tube defects. In seven cases of trisomy 21 and one case of partial trisomy 3, alpha-fetoprotein concentrations were below the reference range but values for a...

journal_title：Clinical chemistry

authors： Buamah PK,Skillen AW,Harrison J,Davison V

更新日期：1985-04-01 00:00:00

abstract：BACKGROUND:Hyperhomocysteinemia (HHcy) has been linked to impaired left ventricular function and clinical class in patients with chronic heart failure. We hypothesized that HHcy stimulates myocardial brain natriuretic peptide (BNP) expression and induces adverse left ventricular remodeling. METHODS:We randomized 50 ra...

journal_title：Clinical chemistry

authors： Herrmann M,Taban-Shoma O,Hübner U,Pexa A,Kilter H,Umanskaya N,Straub RH,Böhm M,Herrmann W

更新日期：2007-04-01 00:00:00

abstract：:It has been hypothesized over many years that the autoimmune endocrine diseases are each due to antigen-specific defects in suppressor (regulatory) T lymphocyte function. There is now increasing evidence in studies of autoimmune thyroid disease and insulin-dependent diabetes mellitus that suppressor cells are activate...

journal_title：Clinical chemistry

authors： Volpé R

更新日期：1994-11-01 00:00:00

abstract：:The commonly used methods of assessing the precision of the automated leukocyte differential have certain drawbacks that affect the validity and comparability of results. In the present report, we introduce a procedure based on building precision profiles from a large number of within-run imprecision experiments. The ...

journal_title：Clinical chemistry

authors： Hübl W,Tlustos L,Bayer PM

更新日期：1996-07-01 00:00:00

abstract：BACKGROUND:Small RNAs are key players in the regulation of gene expression and differentiation. However, many different classes of small RNAs (sRNAs) have been described with distinct biogenesis pathways and, as a result, with different biochemical properties. To analyze sRNAs by deep sequencing, complementary DNA synt...

journal_title：Clinical chemistry

authors： Meistertzheim M,Fehlmann T,Drews F,Pirritano M,Gasparoni G,Keller A,Simon M

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:Plasma B-type cardiac natriuretic peptides reflect cardiac structure and function and have proven roles in assisting in the diagnosis of acute heart failure. They are independent prognostic indicators across the full spectrum of cardiovascular disease. Serial changes in plasma B-type cardiac natriuretic pept...

journal_title：Clinical chemistry

authors： Richards AM,Troughton RW

更新日期：2012-01-01 00:00:00

abstract：:In this paper, we present initial quality-control evaluations for a prototype clinical laboratory, recommendations for clinical and technical data record-keeping, and laboratory protocols designed to minimize operator-dependent analytical error in a multi-user environment. These evaluations and recommendations are bas...

journal_title：Clinical chemistry

authors： Daufeldt JA,Harrison HH

更新日期：1984-12-01 00:00:00

abstract：BACKGROUND:Human iron status is influenced by environmental and genetic factors. We hypothesized that the genetic polymorphism of haptoglobin (Hp), a hemoglobin-binding plasma protein, could affect iron status. METHODS:Reference values of serum iron status markers were compared according to Hp phenotypes (Hp 1-1, Hp 2...

journal_title：Clinical chemistry

authors： Langlois MR,Martin ME,Boelaert JR,Beaumont C,Taes YE,De Buyzere ML,Bernard DR,Neels HM,Delanghe JR

更新日期：2000-10-01 00:00:00

abstract：:Highly purified human lactate dehydrogenases I and V were assayed in 17 different buffers, at a variety of reaction pH's. Diethanolamine and 2-amino-2-methyl-1,3-propanediol provided the best measurements of the enzyme, assayed lactate-to-pyruvate. However, the commercial preparation of 2-amino-2-methyl-1,3-propanedio...

journal_title：Clinical chemistry

authors： Buhl SN,Jackson KY,Lubinski R,Vanderlinde RE

更新日期：1976-11-01 00:00:00