Multimarker quantitative real-time PCR detection of circulating melanoma cells in peripheral blood: relation to disease stage in melanoma patients.

abstract：BACKGROUND:24,25-Dihydroxyvitamin D [24,25(OH)2D] in serum may be both a nuisance and nutritionally valuable. METHODS:We investigated the impact of 24,25(OH)2D3 on the performance of commercially available immunoassays for serum total 25-hydroxyvitamin D [25(OH)D] using (a) serum from a nationally representative sampl...

journal_title：Clinical chemistry

authors： Cashman KD,Hayes A,Galvin K,Merkel J,Jones G,Kaufmann M,Hoofnagle AN,Carter GD,Durazo-Arvizu RA,Sempos CT

更新日期：2015-04-01 00:00:00

abstract：:We describe a mechanized method for centrifugal analyzer determination of sorbitol dehydrogenase in serum, based on conversion of D-fructose to sorbitol with simultaneous oxidation of NADH, in triethanolamine buffer at pH 7.4 and 30 degrees C. The standard curve for this assay is linear to 200 U of activity per liter ...

journal_title：Clinical chemistry

authors： Dooley JF,Turnquist LJ,Racich L

更新日期：1979-12-01 00:00:00

abstract：BACKGROUND:Plant-derived cardenolides reportedly possess anticancer properties in human leukemic cells via selective induction of apoptosis, cell cycle arrest, and differentiation. Selective induction of apoptosis with mammalian-derived digoxin-like immunoreactive factor (DLIF) could provide new strategies for anticanc...

journal_title：Clinical chemistry

authors： Ihenetu K,Qazzaz HM,Crespo F,Fernandez-Botran R,Valdes R Jr

更新日期：2007-07-01 00:00:00

abstract：:Because of uncertainty as to the molecular weight of transferrin, a previous comparison [Von der Heul et al., Clin. Chim. Acta 38, 347 (1972)] between transferrin content of serum and total iron-binding capacity cannot be definitive. We found a conversion factor for expressing the maximum amount of iron bound by 1 mg ...

journal_title：Clinical chemistry

authors： Tsung SH,Rosenthal WA,Milewski KA

更新日期：1975-07-01 00:00:00

abstract：:We evaluated a new agarose-gel-electrophoretic procedure (Corning) (I) for separating and quantitating of high-density lipoprotein cholesterol (HDLC), comparing it with the modified Lipid Research Clinics (LRC) procedure (heparin 183 kilounits/L, MnCl2 92 mmol/L) (II). Method I was insensitive to an HDLC concentration...

journal_title：Clinical chemistry

authors： Stein EA,McNeely S,Steiner P

更新日期：1979-11-01 00:00:00

abstract：:We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be deter...

journal_title：Clinical chemistry

authors： Björkhem I,Blomstrand R,Lantto O,Svensson L,Ohman G

更新日期：1976-11-01 00:00:00

abstract：:We describe an automated assay of glycohemoglobin performed with the Abbott Vision analyzer. The assay is based on batch affinity-extraction with 3-aminophenylboronic acid-derivatized agarose beads. Reagents are packaged in a disposable test pack. Whole-blood specimens are hemolyzed with saponin within a glass capilla...

journal_title：Clinical chemistry

authors： Fiechtner M,Ramp J,England B,Knudson MA,Little RR,England JD,Goldstein DE,Wynn A

更新日期：1992-12-01 00:00:00

abstract：BACKGROUND:Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable met...

journal_title：Clinical chemistry

authors： Pompach P,Nováková J,Kavan D,Benada O,Růžička V,Volný M,Novák P

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:The goal of this study was to establish a plasma microRNA profile by use of next-generation sequencing that could aid in assessment of patient prognosis in nasopharyngeal carcinoma (NPC). METHODS:Two panels of NPC patients and healthy controls (HCs) were recruited for this study. We used deep sequencing to ...

journal_title：Clinical chemistry

authors： Wang HY,Yan LX,Shao Q,Fu S,Zhang ZC,Ye W,Zeng YX,Shao JY

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac t...

journal_title：Clinical chemistry

authors： Pretorius CJ,Dimeski G,O'Rourke PK,Marquart L,Tyack SA,Wilgen U,Ungerer JP

更新日期：2011-05-01 00:00:00

abstract：:Based on the quantitative determination of creatinine, total protein, albumin, alpha 1-microglobulin, IgG, alpha 2-macroglobulin, and N-acetyl-beta, D-glucosaminidase in urine in combination with a test strip screening, the findings of hematuria, leukocyturia, and proteinuria can be assigned to prerenal, renal, or pos...

journal_title：Clinical chemistry

authors： Ivandić M,Hofmann W,Guder WG

更新日期：1996-08-01 00:00:00

abstract：BACKGROUND:Risk assessments of patients should be based on objective variables, such as biological markers that can be measured routinely. The acute response to stress causes the release of catecholamines from the adrenal medulla accompanied by chromogranin A (CGA). To date, no study has evaluated the prognostic value ...

journal_title：Clinical chemistry

authors： Zhang D,Lavaux T,Voegeli AC,Lavigne T,Castelain V,Meyer N,Sapin R,Aunis D,Metz-Boutigue MH,Schneider F

更新日期：2008-09-01 00:00:00

abstract：:We evaluated a new commercially available partially purified reduced bovine hemoglobin solution (RBHS) and a new tonometer for use in the quality control of blood gas analyzers. RBHS is manufactured in three different formulations, each corresponding to three different bicarbonate-buffering capacities and concentratio...

journal_title：Clinical chemistry

authors： Mahoney JJ,Wong RJ,Van Kessel AL

更新日期：1993-05-01 00:00:00

abstract：:This new one-step chemiluminescent immunoassay of free thyroxin (FT4) involving a thyroxin-immunoglobulin conjugate labeled with acridinium ester (Magic Lite System; Ciba Corning Diagnostics Corp., Medfield, MA) is rapid (one 1-h incubation), requires two calibrators per run, and takes 10 s per sample for the quantifi...

journal_title：Clinical chemistry

authors： Bounaud JY,Bounaud MP,Begon F

更新日期：1988-12-01 00:00:00

abstract：BACKGROUND:New genetic forms of hereditary hemochromatosis (HH) or hereditary hyperferritinemia (HF) have been identified over the last few years, and abnormalities of various genes may interact in a single patient. This study aimed to develop a rapid automated method for sequencing the main genes involved. METHODS:We...

journal_title：Clinical chemistry

authors： Cunat S,Giansily-Blaizot M,Bismuth M,Blanc F,Dereure O,Larrey D,Quellec AL,Pouderoux P,Rose C,Raingeard I,Renard E,Schved JF,Aguilar-Martinez P,CHU Montpellier AOI 2004 Working Group.

更新日期：2007-12-01 00:00:00

abstract：BACKGROUND:In recent years, a large number of studies have been published on the performance of glucose monitors. The quality of these reports is not known. METHODS:We searched the PubMed database for performance evaluations of handheld glucose monitors published from August 2002 to November 2006. Relevant articles we...

journal_title：Clinical chemistry

authors： Mahoney J,Ellison J

更新日期：2007-06-01 00:00:00

abstract：:The clinical value of measuring concentrations of methylmalonic acid in serum (S-MMA) as an aid in the diagnosis of cobalamin deficiency has recently aroused interest. In 58 healthy subjects, ages 40-68 years, we found a 0.95 reference interval of 0.05-0.37 mumol/L (mean 0.21, SD 0.094). In 33 of the subjects, who wer...

journal_title：Clinical chemistry

authors： Rasmussen K,Møller J,Ostergaard K,Kristensen MO,Jensen J

更新日期：1990-07-01 00:00:00

abstract：BACKGROUND:Whether low plasma 25-hydroxyvitamin D concentrations cause osteoporotic fractures is unclear. We tested the hypothesis that low plasma 25-hydroxyvitamin D concentrations are associated with increased risk of osteoporotic fractures using a Mendelian randomization analysis. METHODS:We genotyped 116 335 rando...

journal_title：Clinical chemistry

authors： Çolak Y,Afzal S,Nordestgaard BG

更新日期：2020-05-01 00:00:00

abstract：BACKGROUND:Advances in the biological sciences and technology are providing molecular targets for diagnosing and treating cancer. Current classifications in surgical pathology for staging malignancies are based primarily on anatomic features (e.g., tumor-node-metastasis) and histopathology (e.g., grade). Microarrays to...

journal_title：Clinical chemistry

authors： Bernard PS,Wittwer CT

更新日期：2002-08-01 00:00:00

abstract：:We have designed and constructed a digital matrix photometer for quantitative measurement of reflected light of small chromophoric areas or colored spots. The areas are divided conceptually into small subunits in which the reflected light is measured. This is done through stepwise scanning with a photodiode array. The...

journal_title：Clinical chemistry

authors： Neeley WE,Zettner A

更新日期：1983-06-01 00:00:00

abstract：BACKGROUND:Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-...

journal_title：Clinical chemistry

authors： Amicarelli G,Adlerstein D,Shehi E,Wang F,Makrigiorgos GM

更新日期：2006-10-01 00:00:00

abstract：:We determined fluoxetine (Prozac) and its major metabolite norfluoxetine in plasma by liquid chromatography with fluorescence detection. After liquid-liquid extraction from 1 mL of plasma, the extract was derivatized at room temperature with dansyl chloride, and the highly fluorescent derivatives were chromatographed ...

journal_title：Clinical chemistry

authors： Suckow RF,Zhang MF,Cooper TB

更新日期：1992-09-01 00:00:00

abstract：BACKGROUND:The 2447 A > T pathogenic variation at codon 816 of exon 17 (D816V) in the KIT gene, occurring in systemic mastocytosis (SM), leads to constitutive activation of tyrosine kinase activity and confers resistance to the tyrosine kinase inhibitor imatinib mesylate. Thus detection of this variation in SM patients...

journal_title：Clinical chemistry

authors： Tan A,Westerman D,McArthur GA,Lynch K,Waring P,Dobrovic A

更新日期：2006-12-01 00:00:00

abstract：:Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled ...

journal_title：Clinical chemistry

authors： Kienhuis CB,Heuvel JJ,Ross HA,Foekens JA,Benraad TJ

更新日期：1992-05-01 00:00:00

abstract：:Clinicians confront the classical problem of decision making under uncertainty, but a universal procedure by which they deal with this situation, both in diagnosis and therapy, can be defined. This consists in the choice of a specific course of action from available alternatives so as to reduce uncertainty. Formal ana...

journal_title：Clinical chemistry

authors： Werner M

更新日期：1995-08-01 00:00:00

abstract：BACKGROUND:Yersinia pestis, the causative agent of the zoonotic infection plague, is a major concern as a potential bioweapon. Current real-time PCR assays used for Y. pestis detection are based on plasmid targets, some of which may generate false-positive results. METHODS:Using the yp48 gene of Y. pestis, we designed...

journal_title：Clinical chemistry

authors： Chase CJ,Ulrich MP,Wasieloski LP Jr,Kondig JP,Garrison J,Lindler LE,Kulesh DA

更新日期：2005-10-01 00:00:00

abstract：:This enzyme immunoassay procedure for pregnancy-associated plasma protein type A involves a "sandwich"-type system in microtitration plates. One can detect 0.27 mg of the protein per liter of serum, with a between-batch CV of 10.2%, and the antiserum used does not cross react with any of the other placentally derived ...

journal_title：Clinical chemistry

authors： MacDonald DJ,Mehta HC,Mack DS,Duffy T,Glen AC

更新日期：1984-11-01 00:00:00

abstract：:Currently, we can make the following generalizations about the major long-term complications of diabetes. First, they occur commonly after 10 to 15 years of diabetes. Second, the complications of renal and retinal disease, but not of atherogenic and neurological disease, appear to be related to the severity of hypergl...

journal_title：Clinical chemistry

authors： Santiago JV

更新日期：1986-10-01 00:00:00

abstract：BACKGROUND:Altered germline expression of genes may represent a powerful marker of genetic or epigenetic predisposition to cancer or other diseases. METHODS:We developed and validated a method of nonfluorescent primer extension that uses a single dideoxynucleotide and denaturing HPLC (DHPLC) to analyze the relative al...

journal_title：Clinical chemistry

authors： Aceto GM,De Lellis L,Catalano T,Veschi S,Radice P,Di Iorio A,Mariani-Costantini R,Cama A,Curia MC

更新日期：2009-09-01 00:00:00

abstract：:We review atomic flame spectrometry of metals based on the selective laser-enhancement of the rate of atomic ionization. A discussion of basic principles, prior literature, and instrumentation is followed by an overview of analytical figures of merit for the technique, and a look at its present status and areas for fu...

journal_title：Clinical chemistry

authors： Travis JC,Turk GC,DeVoe JR

更新日期：1983-09-01 00:00:00