Abstract:
BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac troponin concurrently in duplicate with 4 analyzers on 2391 samples. The outliers were detected from the difference between duplicate results and by calculating a z value: z = (result 1 - result 2) ÷ √(SD1(est)² + SD2(est)²), with z > 3.48 identifying outliers with a probability of 0.0005. RESULTS:The outlier rates were as follows: Abbott Architect i2000SR STAT Troponin-I, 0.10% (0.01%-0.19%); Beckman Coulter Access2 Enhanced AccuTnI, 0.44% (0.25%-0.63%); Roche Cobas e601 TroponinT hs, 0.06% (0.00%-0.13%); and Siemens ADVIA Centaur XP TnI-Ultra, 0.10% (0.01%-0.19%). The occurrence of outliers was higher than statistically expected on all platforms except the Cobas e601 (χ² = 2.7; P = 0.10). A conservative approach with a constant 10% CV and z > 5.0 identified outliers with clear clinical impact and resulted in outlier rates of 0.11% (0.02%-0.20%) with the Architect i2000SR STAT Troponin-I, 0.36% (0.19%-0.53%) with the Access2 Enhanced AccuTnI, 0.02% (0.00%-0.06%) with the Cobas e601 TroponinT hs, and 0.06% (0.00%-0.13%) with the ADVIA Centaur XP TnI-Ultra. CONCLUSIONS:Outliers occurred on all analytical platforms, at different rates. Clinicians should be made aware by their laboratory colleagues of the existence of outliers and the rate at which they occur.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Pretorius CJ,Dimeski G,O'Rourke PK,Marquart L,Tyack SA,Wilgen U,Ungerer JPdoi
10.1373/clinchem.2010.159830subject
Has Abstractpub_date
2011-05-01 00:00:00pages
710-8issue
5eissn
0009-9147issn
1530-8561pii
clinchem.2010.159830journal_volume
57pub_type
杂志文章abstract:BACKGROUND:It has been shown that a high percentage of interleukin-8 (IL-8) in blood is cell associated. Recently, a simple method for determination of cell-associated IL-8 in whole blood after cell lysis has been described. The purpose of this study was to evaluate this method, to examine the influence of preanalytic ...
journal_title:Clinical chemistry
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doi:
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abstract:BACKGROUND:Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. METHODS:We used layer-by-layer technology ...
journal_title:Clinical chemistry
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doi:10.1373/clinchem.2005.065789
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abstract::We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is rele...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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abstract:BACKGROUND:Human iron status is influenced by environmental and genetic factors. We hypothesized that the genetic polymorphism of haptoglobin (Hp), a hemoglobin-binding plasma protein, could affect iron status. METHODS:Reference values of serum iron status markers were compared according to Hp phenotypes (Hp 1-1, Hp 2...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::We conducted a proficiency survey of cholesterol and high-density lipoprotein (HDL) cholesterol analysis in local clinical laboratories to determine whether increased national emphasis on cholesterol measurement had resulted in changes in performance from previous surveys. Sets of frozen aliquots of plasma and HDL sup...
journal_title:Clinical chemistry
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abstract::We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-12-01 00:00:00
abstract::We compared cotinine, carboxyhemoglobin, and thiocyanate concentrations in blood sampled from 187 cigarette smokers and 181 non-smokers. All three differed significantly between smokers and non-smokers. Cotinine performed best as a test for assessing smoking status, with a sensitivity of 98% as compared with 94% for c...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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abstract::An optimized assay for alkaline phosphatase (EC 3.1.3.1) is reported. A discrete analyzer, the DuPont Automatic Clinical Analyzer (aca), was used for this study. The assay is based on results of response-surface experimental co-optimization techniques, and response is enhanced over the present aca assay. A key feature...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::This communication describes and evaluates an improved routine methodology for quantitating clinical proteinuria. Based on investigations of Piscator and of Savory et al., a modified Tsuchiya's reagent (ethanolic HCI-phosphotungstic acid) is used to precipitate proteins at 56 degrees C, followed by biuret spectrophoto...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-03-01 00:00:00
abstract::Histograms of the results of quantitative clinical laboratory analyses are usually broadly skewed because some of the patients have normal values with respect to the analyte being measured while values for others are below or above normal. We describe a technique for calculating the maximum possible number of individu...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::We compared the ionic activity of sodium, as measured with glass electrodes, with sodium concentration in 23 healthy persons, 15 persons with acute renal failure, and before and after dialysis in 46 patients with chronic renal failure. In healthy persons the mean (+/- SEM) sodium concentration was 139.1 +/- 0.6 mmol/L...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:2010-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2014.221770
更新日期:2015-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-09-01 00:00:00
abstract::The metabolism of the synthetic substrate 7-ethoxyresorufin is a selective measure of the activity of cytochrome P-448 monooxygenase, the subset of cytochrome P-450-mediated enzymes preferentially induced by polycyclic aromatic hydrocarbons and related compounds. 7-Ethoxycoumarin metabolism, on the other hand, reflect...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::We evaluated the density-gradient ultracentrifugation method in a swinging-bucket rotor (Anal Biochem 111, 149-157, 1981) in a slightly modified version for isolation and determination of high-density lipoprotein (HDL) subfractions. We prestained the serum with Coomassie Brilliant Blue R, which did not change the hydr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-04-01 00:00:00
abstract::Antithrombin III (AT III) activity was determined with two different new chromogenic substances--Chromozym-TH (Tos-Gly-Arg-p-nitroanilide; Boehringer Mannheim) and alpha-N-carbobenzyl-oxy-L-lysine-thiobenzyl ester (Du Pont)--with both a discrete (aca) and a centrifugal analyzer (COBAS BIO). The correlation between the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-06-01 00:00:00
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journal_title:Clinical chemistry
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