Accuracy of 6 routine 25-hydroxyvitamin D assays: influence of vitamin D binding protein concentration.

abstract：:Kappa Bence Jones proteinuria was found by immunoelectrophoretic techniques in a patient with plasma cell leukemia, who presented with no M-proteins either in serum or urine. However, a significant decrease in normal immunoglobulins was observed. On microscopic examination of kidney sections obtained a autopsy, protei...

journal_title：Clinical chemistry

authors： Chow C,Spitzer RW

更新日期：1975-10-01 00:00:00

abstract：BACKGROUND:Hypoxic ischemic encephalopathy (HIE) is a major cause of permanent neurological disabilities in full-term newborns. We measured activin A in urine collected immediately after birth in asphyxiated full-term newborns, and assessed the ability of the measurements to predict the occurrence of perinatal encephal...

journal_title：Clinical chemistry

authors： Florio P,Luisi S,Moataza B,Torricelli M,Iman I,Hala M,Hanna A,Petraglia F,Gazzolo D

更新日期：2007-03-01 00:00:00

abstract：:Changes in values for myoglobin, total creatine kinase (EC 2.7.3.2), and creatine kinase B-subunit in the serum of patients with thyroid disease are compared with values for these during the 24-h after myocardial infarction. Concentrations of all three of these muscle-derived proteins were significantly higher than no...

journal_title：Clinical chemistry

authors： Docherty I,Harrop JS,Hine KR,Hopton MR,Matthews HL,Taylor CJ

更新日期：1984-01-01 00:00:00

abstract：:This time-resolved immunofluorometric assay (IFMA) developed for measurement of placental protein 5 (PP5) involves two antibodies: a monoclonal anti-PP5 antibody attached to a solid phase and an europium(III) chelate-labeled polyclonal anti-PP5 antibody as a tracer. The measuring range is 0.05-100 micrograms/L and the...

journal_title：Clinical chemistry

authors： Bützow R,Alfthan H,Stenman UH,Suikkari AM,Bohn H,Seppälä M

更新日期：1988-08-01 00:00:00

abstract：:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin ...

journal_title：Clinical chemistry

authors： Jortani SA,Helm RA,Valdes R Jr

更新日期：1996-10-01 00:00:00

abstract：:We assessed the accuracy and precision of seven commercial kits for serum ferritin. The concentration of ferritin as determined by these assays for a liver ferritin reference standard, a human heart ferritin standard, and normal sera correlated highly, but the absolute values varied widely. Use of an international ref...

journal_title：Clinical chemistry

authors： Iacobello C,Ghielmi S,Belloli S,Arosio P,Albertini A

更新日期：1984-02-01 00:00:00

abstract：:We describe the use of electron-capture gas chromatography or reversed-phase "high-performance" liquid chromatography for concurrent analysis for diazepam and nordiazepam in serum. In the gas-chromatographic analysis our use of a new chemically deactivated stationary liquid phase, SP 2250-DB (Supelco, Inc.), resulted ...

journal_title：Clinical chemistry

authors： Wallace JE,Schwertner HA,Shimek EL Jr

更新日期：1979-07-01 00:00:00

abstract：:Endotoxin, the lipopolysaccharide cell wall constituent of Gram-negative bacteria, produces symptoms of the Gram-negative sepsis syndrome. By measuring endotoxin in blood from septic patients it may be possible to select a subpopulation of patients in which mortality can be prevented by treatment with anti-endotoxin a...

journal_title：Clinical chemistry

authors： Salden HJ,Bas BM

更新日期：1994-08-01 00:00:00

abstract：:We describe a sensitive and precise gas-chromatographic method, in which cytosine is used as the internal standard, for determination of an antifungal agent, 5-fluorocytosine, in serum. The trimethylsilyl derivative of this drug is well separated from the internal standard and from normal serum constituents. Amphoteri...

journal_title：Clinical chemistry

authors： Harding SA,Johnson GF,Solomon HM

更新日期：1976-06-01 00:00:00

abstract：:A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include...

journal_title：Clinical chemistry

authors： Bais R,Edwards JB

更新日期：1976-12-01 00:00:00

abstract：:Measurement of serum ascorbate may be useful in long-term population studies because of the possible influence of ascorbate on numerous physiological factors. We describe an automated method for determining ascorbate in serum and urine by using the reduction of ferric iron by ascorbate and the formation of a color bet...

journal_title：Clinical chemistry

authors： Butts WC,Mulvihill HJ

更新日期：1975-09-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal testing (NIPT) uses cell-free DNA (cfDNA) as an analyte to detect copy-number alterations in the fetal genome. Because maternal and fetal cfDNA contributions are comingled, changes in the maternal genome can manifest as abnormal NIPT results. Circulating tumor DNA (ctDNA) present in case...

journal_title：Clinical chemistry

authors： Dharajiya NG,Grosu DS,Farkas DH,McCullough RM,Almasri E,Sun Y,Kim SK,Jensen TJ,Saldivar JS,Topol EJ,van den Boom D,Ehrich M

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required. METHODS:We evaluated extracti...

journal_title：Clinical chemistry

authors： Bohmann K,Hennig G,Rogel U,Poremba C,Mueller BM,Fritz P,Stoerkel S,Schaefer KL

更新日期：2009-09-01 00:00:00

abstract：BACKGROUND:Mitochondrial diseases are clinically and genetically heterogeneous, with variable penetrance, expressivity, and differing age of onset. Disease-causing point mutations and large deletions in the mitochondrial genome often exist in a heteroplasmic state. Current molecular analyses require multiple different ...

journal_title：Clinical chemistry

authors： Zhang W,Cui H,Wong LJ

更新日期：2012-09-01 00:00:00

abstract：:Immunoradiometric analysis has made it possible to measure ferritin reliably in serum. The determination is a sensitive means of assessing body iron because concentrations of ferritin closely parallel the body's reticuloendothelial iron stores. The information provided is similar to that obtained from bone-marrow aspi...

journal_title：Clinical chemistry

authors： Forman DT,Vye MV

更新日期：1980-01-01 00:00:00

abstract：BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation ac...

journal_title：Clinical chemistry

authors： Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret P

更新日期：2007-10-01 00:00:00

abstract：BACKGROUND:Risk assessments of patients should be based on objective variables, such as biological markers that can be measured routinely. The acute response to stress causes the release of catecholamines from the adrenal medulla accompanied by chromogranin A (CGA). To date, no study has evaluated the prognostic value ...

journal_title：Clinical chemistry

authors： Zhang D,Lavaux T,Voegeli AC,Lavigne T,Castelain V,Meyer N,Sapin R,Aunis D,Metz-Boutigue MH,Schneider F

更新日期：2008-09-01 00:00:00

abstract：BACKGROUND:Efforts have been undertaken recently to assess the fetal genome through analysis of circulating cell-free (ccf) fetal DNA obtained from maternal plasma. Sequencing analysis of such ccf DNA has been shown to enable accurate prenatal detection of fetal aneuploidies, including trisomies of chromosomes 21, 18, ...

journal_title：Clinical chemistry

authors： Jensen TJ,Dzakula Z,Deciu C,van den Boom D,Ehrich M

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:D-2-Hydroxyglutaric aciduria (D-2-HGA), L-2-hydroxyglutaric aciduria (L-2-HGA), and the combined D/L-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-...

journal_title：Clinical chemistry

authors： Struys EA,Verhoeven NM,Roos B,Jakobs C

更新日期：2003-07-01 00:00:00

abstract：:We describe the influence of autoantibodies that bind creatine kinase BB (CK-BB) on the methods for MB isoenzyme. If these autoantibodies are present in patients' sera, they cause the formation of macro CK type 1 (immunoglobulin-linked CK-BB). In some of these cases they can bind not only endogenous CK-BB but also CK-...

journal_title：Clinical chemistry

authors： Stein W,Bohner J

更新日期：1985-07-01 00:00:00

abstract：BACKGROUND:False-positive results are a common problem in real-time PCR identification of DNA sequences that differ from near neighbors by a single-nucleotide polymorphism (SNP) or deletion. Because of a lack of sufficient probe specificity, post-PCR analysis, such as a melting curve, is often required for mutation dif...

journal_title：Clinical chemistry

authors： Satterfield BC,Kulesh DA,Norwood DA,Wasieloski LP Jr,Caplan MR,West JA

更新日期：2007-12-01 00:00:00

abstract：:During direct illumination of a serum bilirubin solution the bilirubin concentration decreased markedly, both as determined by the direct spectrometric method and (even more so) by the diazo method. In contrast, I found the same values for serum bilirubin concentrations as determined by these two methods for serum fro...

journal_title：Clinical chemistry

authors： Ebbesen F

更新日期：1977-01-01 00:00:00

abstract：BACKGROUND:Astilbin is a flavonoid isolated from the rhizome of Smilax glabra. In a previous study, we revealed its unique immunosuppressive activity, a selective inhibition against activated T lymphocytes. This characteristic of astilbin is beneficial for the treatment of human immune diseases. METHODS:We incubated a...

journal_title：Clinical chemistry

authors： Guo J,Qian F,Li J,Xu Q,Chen T

更新日期：2007-03-01 00:00:00

abstract：:For 51 liver allograft recipients, we evaluated whether serum profiles of delta (Bd) and conjugated bilirubins (Bc) could be used to diagnose rejection during the first 30-50 postoperative days, in comparison with histology as the "gold standard." Daily measurements of aspartate aminotransferase, alkaline phosphatase,...

journal_title：Clinical chemistry

authors： Wu TW,Levy GA,Yiu S,Au JX,Greig PD,Strasberg SM,Ettles M,Abecassis M,Superina RA,Langer B

更新日期：1990-01-01 00:00:00

abstract：:In radioimmunoassay of digoxin by the dextran-coated charcoal method, duration of the incubation period for the antigen-antibody reaction and time allowed for dextran-coated charcoal to absorb the free antigen are critical factors. We compared effects of prolonged periods of absorption on results obtained with five co...

journal_title：Clinical chemistry

authors： Kuno-Sakai H,Sakai H

更新日期：1975-02-01 00:00:00

abstract：BACKGROUND:TMPRSS2:ERG fusions are promising prostate cancer biomarkers. Because they can occur in multiple forms in a single cancer specimen, we developed a quantitative PCR test that detects both type III and type VI TMPRSS2:ERG fusions. The assay is quantified from a standard curve determined with a plasmid-cloned t...

journal_title：Clinical chemistry

authors： Clark JP,Munson KW,Gu JW,Lamparska-Kupsik K,Chan KG,Yoshida JS,Kawachi MH,Crocitto LE,Wilson TG,Feng Z,Smith SS

更新日期：2008-12-01 00:00:00

abstract：:By using bivariate probability estimation for the diagnosis of acute myocardial infarction (AMI) we show how to overcome the difficulties encountered for patients whose clinical presentation is atypical and those encountered when multiple isoenzyme determinations are treated by univariate methods. We use the values fo...

journal_title：Clinical chemistry

authors： Bernstein LH,Good IJ,Holtzman GI,Deaton ML,Babb J

更新日期：1989-03-01 00:00:00

abstract：BACKGROUND:Neuroblastoma is the most common extracranial malignant solid tumor in children under 5 years and is characterized by a wide clinical and biological heterogeneity, from spontaneously regressive forms to cancers with a rapid and fatal progression. MYCN oncogene amplification is considered the most important p...

journal_title：Clinical chemistry

authors： Raggi CC,Bagnoni ML,Tonini GP,Maggi M,Vona G,Pinzani P,Mazzocco K,De Bernardi B,Pazzagli M,Orlando C

更新日期：1999-11-01 00:00:00

abstract：:At the clinical level, we have seen the emergence of a subspecialty of affective disorders within psychiatry. Mood clinics, depression units, and affective disorders centers are appearing in many academic and clinical settings, where clinical skill and knowledge can be concentrated and new research furthered. By using...

journal_title：Clinical chemistry

authors： Klerman GL,Weissman MM

更新日期：1988-05-01 00:00:00

abstract：:Serial measurements of the bone and fetal intestinal isoenzymes of alkaline phosphatase (EC 3.1.3.1) in the plasma of 43 term and 43 preterm infants, from birth to six weeks later, indicate that the bone isoenzyme gradually increases over this period in both preterm and term infants fed with unsupplemented commercial ...

journal_title：Clinical chemistry

authors： Crofton PM,Hume R

更新日期：1987-10-01 00:00:00