Abstract:
:A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include maintaining the pH between 5.6 and 5.9 and the addition of detergent to sustain linearity. The results obtained with alpha-naphthyl phosphate have been compared with those obtained by using p-nitrophenyl phosphate as substrate. When used with an automatic rate analyzer, the modified method is as sensitive but more reproducible.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Bais R,Edwards JBsubject
Has Abstractpub_date
1976-12-01 00:00:00pages
2025-8issue
12eissn
0009-9147issn
1530-8561journal_volume
22pub_type
杂志文章abstract:BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.078980
更新日期:2007-01-01 00:00:00
abstract::For normal and above-normal concentrations of angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity in plasma, results of a manual fluorometric method [with hippuryl-histidyl-leucine (HHL), 5 mmol/L, as substrate] correlated well with those of an automated spectrophotometric method [with 3-(2-furylacryloyl)-L-phen...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::The biological variability of total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and calculated low-density lipoprotein cholesterol (LDL-C) was determined in three serial (monthly) capillary and venous specimens from 83 subjects. The analytes were quantified with a desktop analyz...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::The identification of nicotinamide-N1-oxide as a metabolite in the urine of a schizophrenic patient prompted a study of the relative metabolism of nicotinic acid and nicotinamide in mental patients and healthy volunteers. Metabolites quantified included N1-methyl-2-pyridone-5-carboxamide, N1-methyl-4-pyridone-3-carbox...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract:BACKGROUND:We evaluated the completeness of reporting of diagnostic test accuracy (DTA) systematic reviews using the recently developed Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA)-DTA guidelines. METHODS:MEDLINE® was searched for DTA systematic reviews published October 2017 to January 2...
journal_title:Clinical chemistry
pub_type: 杂志文章,meta分析
doi:10.1373/clinchem.2018.292987
更新日期:2019-02-01 00:00:00
abstract:BACKGROUND:Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagnosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and childr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2005.052167
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Single-nucleotide polymorphisms (SNPs) in inflammation-related genes have been linked to an increased risk of ischemic stroke. Most of these SNP results have not been replicated, however, and metaanalyses of the effects of inflammation-related genes are rare. We investigated 49 SNPs in 34 genes previously re...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.112151
更新日期:2009-01-01 00:00:00
abstract::We present a comparative evaluation of two commercial kits, the "Quantitope" and "GammaCoat," for radioimmunoassay of digoxin in serum. These kits, in which iodine-125 is used as a label, proved to suitable for digoxin assay as determined by their reproducibility, sensitivity, precision, and a regression analysis. Hem...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-02-01 00:00:00
abstract:BACKGROUND:High circulating concentrations of N-terminal fragments of A- and B-type natriuretic peptides (NT-proANP and NT-proBNP) identify patients with impaired cardiac function. ProANP-derived peptides are particularly sensitive to increased preload of the heart and proBNP-derived peptides to increased afterload; th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.039891
更新日期:2005-04-01 00:00:00
abstract::The commonly used methods of assessing the precision of the automated leukocyte differential have certain drawbacks that affect the validity and comparability of results. In the present report, we introduce a procedure based on building precision profiles from a large number of within-run imprecision experiments. The ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilita...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::We describe the influence of autoantibodies that bind creatine kinase BB (CK-BB) on the methods for MB isoenzyme. If these autoantibodies are present in patients' sera, they cause the formation of macro CK type 1 (immunoglobulin-linked CK-BB). In some of these cases they can bind not only endogenous CK-BB but also CK-...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::Abnormal creatine kinase (CK) isoenzyme patterns were observed in the serum of a 64-year-old woman with severe heart disease. Agarose electrophoresis revealed the presence of all the usual CK isoenzymes (MM, MB, and BB) plus an extra band between MM and MB. Total serum CK activity was within the normal range. Within 2...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-10-01 00:00:00
abstract:BACKGROUND:A reduced response to aspirin and clopidogrel predicts ischemic events, but reliable tests are needed to identify low responders. We compared 3 platelet-function tests during long-term dual treatment with aspirin and clopidogrel. METHODS:Patients who underwent a percutaneous coronary intervention and were r...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2009.137471
更新日期:2010-05-01 00:00:00
abstract::We describe a high-performance liquid chromatographic method for the quantitative determination of gentamicin in serum. The antibiotic was separated from serum by passage through a silicic acid column, derivatized with o-phthalaldehyde, and eluted with ethanol. The derivatized gentamicin was then separated into all th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-12-01 00:00:00
abstract::Apoprotein (apo) C-I, C-II, and C-III were measured in plasma from 35 white American non-diabetic subjects. The mean concentrations (mg/L) in plasma from normolipidemic and hypertriglyceridemic subjects, respectively, were apo C-I, 78 and 311; apo C-II, 37 and 200; and apo C-III, 98 and 529. These concentrations were ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::Numerous xenobiotics regulate cellular functions by altering transcription of target genes. Use of sensitive and specific biomarkers based on gene transcript levels may help clarify the shape of the dose-response curve in the low-dose region associated with human exposures to environmental concentrations of chemicals....
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:
更新日期:1995-12-01 00:00:00
abstract:BACKGROUND:FMR1 full mutations (FMs) (CGG expansion >200) in males mosaic for a normal (<45 CGG) or gray-zone (GZ) (45-54 CGG) allele can be missed with the standard 2-step fragile X syndrome (FXS) testing protocols, largely because the first-line PCR tests showing a normal or GZ allele are not reflexed to the second-l...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2015.244681
更新日期:2016-02-01 00:00:00
abstract::The manual Reference Method of the Centers for Disease Control for serum iron (CDC/RM/Fe) and a semiautomated adaptation of it were used to evaluate two working methods: one, a detergent solubilization procedure for the Roche Cobas-Bio analyzer, the other, the Kodak Ektachem 700 procedure, based on dry-film technology...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-10-01 00:00:00
abstract::We compared a chemiluminescent assay and a colorimetric endpoint assay for measuring an alkaline phosphatase (EC 3.1.3.1) label in an enzyme immunoassay of thyrotropin (TSH). The substrate in the chemiluminescent assay is a derivative of adamantyl 1,2-dioxetane phosphate. On dephosphorylation, catalyzed by alkaline ph...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-07-01 00:00:00
abstract:BACKGROUND:Adequate detection of designer steroids in the urine of athletes is still a challenge in doping control analysis and requires knowledge of steroid metabolism. In this study we investigated whether uPA(+/+)-SCID mice carrying functional primary human hepatocytes in their liver would provide a suitable alterna...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.119396
更新日期:2009-10-01 00:00:00
abstract::Backpropagation neural networks are a computer-based pattern-recognition method that has been applied to the interpretation of clinical data. Unlike rule-based pattern recognition, backpropagation networks learn by being repetitively trained with examples of the patterns to be differentiated. We describe and analyze t...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1993-09-01 00:00:00
abstract::We report a simple, enzymatic, end point method for determining nitrate in serum and urine with use of nitrate reductase from Aspergillus sp. (EC 1.6.6.2). The decrease in absorbance at 340 nm as a result of the oxidation of beta-NADPH is measured. We used FAD as a supplementary electron carrier and added an internal ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::In this liquid-chromatographic method for the determination of bromide in human serum, an important and unique feature is pre-analysis ultrafiltration of the sample, which effectively removes proteins. The chromatographic system consists of an anion-exchange column (Whatman Partisil SAX 10-micron particles), an isocra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-05-01 00:00:00
abstract::We coupled a kinetic procedure to a selective inhibiting method for determining amylase isoenzymes in biological samples, using 4-nitrophenylmaltopentaoside plus 4-nitrophenylmaltohexaoside as substrate and a wheat-germ selective inhibitor with the Gilford S-III spectrophotometer. On plotting remaining amylase activit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract:BACKGROUND:Stability of chemical speciation during sample handling and storage is a prerequisite to obtaining reliable results of trace element speciation analysis. There is no comprehensive information on the stability of common arsenic species, such as inorganic arsenite [As(III)], arsenate [As(V)], monomethylarsonic...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract::We determined approximately 15,000 laboratory values in 236 individuals between the ages of 60 and 90 y, 22 individuals between 90 and 99 y, and 69 individuals greater than or equal to 100 y, and compared these with values in young adults. We tested 47 different analytes in the 60-90-y group and 93 analytes in the gre...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-06-01 00:00:00
abstract::Two-dimensional electroimmunodiffusion is rarely used in clinical chemistry, because its potentialities are not yet widely appreciated. Studying mouse anaphylaxis, we observed with this technique certain striking short- and long-term pathologic changes in several important serum antigens, which aptly demonstrate its u...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::We produced and characterized several monoclonal antibodies directed toward human plasma apolipoprotein A-I. Two of them, A-I-12 and A-I-57, individually precipitated purified or native high-density lipoprotein in agarose gel by double immunodiffusion. Because radial immunodiffusion performed with a single monoclonal ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this me...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00