An optimized continuous-monitoring procedure for semiautomated determination of serum acid phosphatase activity.

Abstract:

:A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include maintaining the pH between 5.6 and 5.9 and the addition of detergent to sustain linearity. The results obtained with alpha-naphthyl phosphate have been compared with those obtained by using p-nitrophenyl phosphate as substrate. When used with an automatic rate analyzer, the modified method is as sensitive but more reproducible.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Bais R,Edwards JB

subject

Has Abstract

pub_date

1976-12-01 00:00:00

pages

2025-8

issue

12

eissn

0009-9147

issn

1530-8561

journal_volume

22

pub_type

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