Abstract：
:A highly sensitive two-site sandwich ELISA measuring total proinsulin immunoreactive material in serum or plasma was developed. The assay was based on two monoclonal antibodies, an anti-C-peptide antibody bound to a microtest plate and a biotin-labeled anti-insulin antibody. The detection limit (3 SD above zero value) in buffer was 0.05 pmol/L, corresponding to 0.25 pmol/L in human serum (diluted 1:5). The linear calibrator range was 0.05-20 pmol/L. Interassay CVs were 4.7% at a median (range) of 2.3 pmol/L (1.4-2.8 pmol/L, n = 8), 6.7% at 5.1 pmol/L (3.3-8.0 pmol/L, n = 8), and 8.7% at 10.0 pmol/L (8-12 pmol/L, n = 10). Mean analytical recovery of added human proinsulin (hPI) (2, 5, and 10 pmol/L) to serum was 84% (range 68-128%, n = 9). Human insulin and human C-peptide did not cross-react at 5000 and 10,000 pmol/L, respectively. The four major proinsulin conversion intermediates reacted 65-99%: split(32-33)hPI 74%, des-(31,32)hPI 65%, split(65-66)hPI 78%, and des(64,65)hPI 99%. All serum values from 38 fasting healthy subjects were above the detection limit: median (range) 4.0 (2.1-12.6) pmol/L.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Kjems LL,Røder ME,Dinesen B,Hartling SG,Jørgensen PN,Binder Csubject
Has Abstractpub_date
1993-10-01 00:00:00pages
2146-50issue
10eissn
0009-9147issn
1530-8561journal_volume
39pub_type
杂志文章abstract：:Using a continuous-flow (Technicon SMAC) analyzer, we measured the concentration of 20 analytes in capillary blood sera from 149 neonates in an intensive-care unit. Included were term and pre-term babies, both groups with and without asphyxia. The values obtained, especially for those enzymes and analytes that are rel...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilita...
journal_title：Clinical chemistry
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doi：
更新日期：1976-08-01 00:00:00
abstract：:A sensitive method, inductively coupled plasma atomic emission spectroscopy, is used to measure desferrioxamine in blood plasma. The desferrioxamine is transformed into its iron chelate, ferrioxamine, which is extracted into benzyl alcohol, then re-extracted into HCl (0.5 mol/L), which is used as the sample for the sp...
journal_title：Clinical chemistry
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doi：
更新日期：1987-01-01 00:00:00
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doi：10.1373/clinchem.2013.214213
更新日期：2014-05-01 00:00:00
abstract：BACKGROUND:This Case Conference reviews the normal changes in thyroid activity that occur during pregnancy and the proper use of laboratory tests for the diagnosis of thyroid dysfunction in the pregnant patient. CASE:A woman in the 18th week of pregnancy presented with tachycardia, increased blood pressure, severe vom...
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journal_title：Clinical chemistry
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更新日期：1989-07-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章,评审
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journal_title：Clinical chemistry
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doi：
更新日期：1987-07-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1977-10-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2018.301291
更新日期：2019-06-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章,多中心研究
doi：
更新日期：2002-09-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-11-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.099424
更新日期：2008-07-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1994-09-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1998-01-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
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doi：
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doi：
更新日期：2002-11-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1996-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
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journal_title：Clinical chemistry
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doi：10.1373/clinchem.2010.156976
更新日期：2011-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-10-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1977-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-03-01 00:00:00
