Microplate measurement of urinary albumin and creatinine.

abstract：BACKGROUND:The aim of our present study was to establish new reference intervals for thyrotropin (TSH) and thyroid hormones based on National Academy of Clinical Biochemistry (NACB) criteria and regular thyroid ultrasonography. We also assessed the effect of potentially confounding factors to modulate the limits of the...

journal_title：Clinical chemistry

authors： Kratzsch J,Fiedler GM,Leichtle A,Brügel M,Buchbinder S,Otto L,Sabri O,Matthes G,Thiery J

更新日期：2005-08-01 00:00:00

abstract：:We have developed and thoroughly validated radioimmunoassays (RIAs) for osteocalcin in plasma and serum, demonstrating independence of volume and determining the recovery of added standard and within- and between-assay precision. Antibodies were raised in rabbits and mice by immunization with either osteocalcin adsorb...

journal_title：Clinical chemistry

authors： Power MJ,Gosling JP,Fottrell PF

更新日期：1989-07-01 00:00:00

abstract：BACKGROUND:Fetuin-A inhibits inflammation and has a protective effect against myocardial ischemia. Its deficiency has been found to be associated with cardiovascular death in patients with end-stage renal failure disease. We investigated the association between plasma fetuin-A and clinical outcome after ST-elevation ac...

journal_title：Clinical chemistry

authors： Lim P,Collet JP,Moutereau S,Guigui N,Mitchell-Heggs L,Loric S,Bernard M,Benhamed S,Montalescot G,Randé JL,Guéret P

更新日期：2007-10-01 00:00:00

abstract：:A coated microtiter-well, enzyme-linked immunometric assay for quantifying immunoreactive trypsinogen in dried blood spots was modified to use time-resolved fluorescence of europium in place of end-point enzymatic color development as the quantification step. The streptavidin-horseradish peroxidase and color developme...

journal_title：Clinical chemistry

authors： Ryall RG,Gjerde EM,Gerace RL,Ranieri E

更新日期：1993-02-01 00:00:00

abstract：BACKGROUND:Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in i...

journal_title：Clinical chemistry

authors： Campbell DJ,Nussberger J,Stowasser M,Danser AH,Morganti A,Frandsen E,Ménard J

更新日期：2009-05-01 00:00:00

abstract：:In this immunoenzymatic assay for human lutropin (hLH) we used bi-specific antibodies (BiAbs) obtained from the fusion of two hybridomas producing antibodies to beta-D-galactosidase and hLh. The BiAb complexed with the enzyme beta-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay ...

journal_title：Clinical chemistry

authors： Bugari G,Poiesi C,Beretta A,Ghielmi S,Albertini A

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Cardiac troponin T (cTnT) is the preferred biomarker for the diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT is present predominantly in fragmented forms in human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using West...

journal_title：Clinical chemistry

authors： Streng AS,de Boer D,van Doorn WP,Bouwman FG,Mariman EC,Bekers O,van Dieijen-Visser MP,Wodzig WK

更新日期：2017-02-01 00:00:00

abstract：:Measurement of acetaminophen by analysis of the second derivative of its spectrum is specific and sensitive. The method of extraction and the use of just one phosphate buffer as reagent makes this method very convenient. Readings are reliable from 10 to 1500 mg/L. A turnaround time of 20 min makes this method well sui...

journal_title：Clinical chemistry

authors： Dingeon B,Charvin MA,Quenard MT,Thome H

更新日期：1988-06-01 00:00:00

abstract：BACKGROUND:Non-(1-84) parathyroid hormone (PTH) fragments are large C-terminal fragments of PTH with a partially preserved N-terminal structure. They differ from other C-terminal PTH fragments, which do not have an N-terminal structure and do not react in intact PTH assays. We aimed to identify the minimal N-terminal s...

journal_title：Clinical chemistry

authors： D'Amour P,Brossard JH,Räkel A,Rousseau L,Albert C,Cantor T

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Efforts to reduce the high global prevalence of nutritional anemia require the use of both reliable laboratory assays to distinguish iron deficiency from other causes of anemia and cost-effective methods for collection of blood specimens under field conditions. The suitability of using small plasma samples s...

journal_title：Clinical chemistry

authors： Flowers CH,Cook JD

更新日期：1999-10-01 00:00:00

abstract：BACKGROUND:Quantitative, miniaturized nucleic acid assays and immunoassays can be developed with single microparticles, microfluorometric detection, and intrinsically fluorescent lanthanide chelates in a multiple assay format to decrease reagent consumption, cost, and assay time. We used recombinant Fab fragments to ca...

journal_title：Clinical chemistry

authors： Härmä H,Tarkkinen P,Soukka T,Lövgren T

更新日期：2000-11-01 00:00:00

abstract：:We examined the mechanism by which hypertriglyceridemia interferes with the Roche Diagnostics "Amylochrome" procedure for measurement of amylase in lactescent samples with normal and above-normal amylase activity. The serum blank recommended in the Amylochrome protocol to compensate for lactescence remains inappropria...

journal_title：Clinical chemistry

authors： Wickus GG,Dukerschein RO,Pierce JR,Davis KD

更新日期：1982-10-01 00:00:00

abstract：BACKGROUND:Hyperhomocysteinemia (HHcy) has been linked to impaired left ventricular function and clinical class in patients with chronic heart failure. We hypothesized that HHcy stimulates myocardial brain natriuretic peptide (BNP) expression and induces adverse left ventricular remodeling. METHODS:We randomized 50 ra...

journal_title：Clinical chemistry

authors： Herrmann M,Taban-Shoma O,Hübner U,Pexa A,Kilter H,Umanskaya N,Straub RH,Böhm M,Herrmann W

更新日期：2007-04-01 00:00:00

abstract：:Quinidine in serum was measured at 330 nm, after protein precipitation, with an alkyl phenyl reversed-phase chromatographic column by peak-height determination (external standards.) Sensitivity was 0.3 mg/liter, with linear response to at least 10 mg/liter serum. Interassay precision, measured on 20 consecutive days, ...

journal_title：Clinical chemistry

authors： Powers JL,Sadee W

更新日期：1978-02-01 00:00:00

abstract：:The enzymatic determination of serum uric acid by use of uricase, catalase, and aldehyde dehydrogenase according to Haeckel [J. Clin. Chem. Clin Biochem. 14, 101 (1976)] showed interferences from ethanol-converting enzymes, which are present in some patients' sera. We have identified these enzymes as alcohol dehydroge...

journal_title：Clinical chemistry

authors： Bartl K,Brandhuber M,Ziegenhorn J

更新日期：1979-04-01 00:00:00

abstract：:Intra-erythrocytic pH, ATP concentrations, and 2,3-diphosphoglycerate relaxation times were studied in living erythrocytes by "high-resolution" 31P NMR spectroscopy to assess homeostasis within the cells. In uremic patients, intra-erythrocytic pH is significantly decreased before hemodialysis, but is corrected equally...

journal_title：Clinical chemistry

authors： Monti JP,Gallice P,Baas M,Murisasco A,Crevat A

更新日期：1987-01-01 00:00:00

abstract：BACKGROUND:Disease-defining chromosomal translocations are seen in various neoplasms, especially in lymphomas and leukemias. Translocation detection at the DNA level is often complicated by chromosomal breakpoints that are distributed over very large regions. We have developed a ligation-based assay [the looped ligatio...

journal_title：Clinical chemistry

authors： Harada S,Sizzle E,Lin MT,Gocke CD

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:C-reactive protein (CRP) increases in response to inflammation and is purported to be a risk factor for atherogenesis. We recently demonstrated that a scavenger receptor, lectin-like oxidized LDL receptor (LOX-1), is a receptor for CRP. In light of the overlapping ligand spectrum of scavenger receptors such ...

journal_title：Clinical chemistry

authors： Fujita Y,Kakino A,Harada-Shiba M,Sato Y,Otsui K,Yoshimoto R,Sawamura T

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:Cyclosporin A (CsA) is commonly measured by immunoassay techniques that have a limited analytical range. The consequence of this is that low CsA concentrations that may be clinically significant are difficult to measure and that high concentrations require sample dilution, which introduces error and increase...

journal_title：Clinical chemistry

authors： Keevil BG,Tierney DP,Cooper DP,Morris MR

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH). METHODS:After addition of sphinga...

journal_title：Clinical chemistry

authors： Groener JE,Poorthuis BJ,Kuiper S,Helmond MT,Hollak CE,Aerts JM

更新日期：2007-04-01 00:00:00

abstract：BACKGROUND:TMPRSS2:ERG fusions are promising prostate cancer biomarkers. Because they can occur in multiple forms in a single cancer specimen, we developed a quantitative PCR test that detects both type III and type VI TMPRSS2:ERG fusions. The assay is quantified from a standard curve determined with a plasmid-cloned t...

journal_title：Clinical chemistry

authors： Clark JP,Munson KW,Gu JW,Lamparska-Kupsik K,Chan KG,Yoshida JS,Kawachi MH,Crocitto LE,Wilson TG,Feng Z,Smith SS

更新日期：2008-12-01 00:00:00

abstract：:We compared relative increases in creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) after reperfusion in myocardial infarction for four popular methods: electrophoresis, immunoinhibition, the "Magic Lite" (Ciba-Corning) system, and the Stratus (Dade). In a method comparison study, we confirmed that all four methods co...

journal_title：Clinical chemistry

authors： Christenson RH,Clemmensen P,Ohman EM,Toffaletti J,Silverman LM,Grande P,Vollmer RT,Wagner GS

更新日期：1990-08-01 00:00:00

abstract：:Urinary polyamines were measured by a fluorometric thin-layer chromatographic technique in samples collected daily throughout a single menstrual cycle by each of 13 healthy women. Polyamine excretion fluctuated considerably, but excretion of putrescine, spermidine, and spermine combined was consistently greatest durin...

journal_title：Clinical chemistry

authors： Osterberg S,Rosén S,Heby O

更新日期：1978-05-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal diagnosis of monogenic disorders using maternal plasma and targeted massively parallel sequencing is being investigated actively. We previously demonstrated that comprehensive genetic diagnosis of a Duchenne muscular dystrophy (DMD) patient is feasible using a single targeted sequencing ...

journal_title：Clinical chemistry

authors： Yoo SK,Lim BC,Byeun J,Hwang H,Kim KJ,Hwang YS,Lee J,Park JS,Lee YS,Namkung J,Park J,Lee S,Shin JY,Seo JS,Kim JI,Chae JH

更新日期：2015-06-01 00:00:00

abstract：BACKGROUND:Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary refere...

journal_title：Clinical chemistry

authors： van der Vorm LN,Hendriks JC,Laarakkers CM,Klaver S,Armitage AE,Bamberg A,Geurts-Moespot AJ,Girelli D,Herkert M,Itkonen O,Konrad RJ,Tomosugi N,Westerman M,Bansal SS,Campostrini N,Drakesmith H,Fillet M,Olbina G,Pasricha

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:Human herpesvirus 6 (HHV-6) latently infects a majority of adults. In about 1% of the population HHV-6 exists in a chromosomally integrated form (ciHHV-6) that resides in every somatic and germ cell and can be transmitted through the germ line. Patients with ciHHV-6 have been misdiagnosed and unnecessarily t...

journal_title：Clinical chemistry

authors： Sedlak RH,Cook L,Huang ML,Magaret A,Zerr DM,Boeckh M,Jerome KR

更新日期：2014-05-01 00:00:00

abstract：:An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and alpha-ketoglutarate on the other. The rate of disappearance of NADH flu...

journal_title：Clinical chemistry

authors： Kuan JC,Lau KY,Guilbault GG

更新日期：1975-01-01 00:00:00

abstract：BACKGROUND:Efforts have been undertaken recently to assess the fetal genome through analysis of circulating cell-free (ccf) fetal DNA obtained from maternal plasma. Sequencing analysis of such ccf DNA has been shown to enable accurate prenatal detection of fetal aneuploidies, including trisomies of chromosomes 21, 18, ...

journal_title：Clinical chemistry

authors： Jensen TJ,Dzakula Z,Deciu C,van den Boom D,Ehrich M

更新日期：2012-07-01 00:00:00

abstract：:We have developed a succinyldicholine-based assay for serum cholinesterase (EC 3.1.1.8) to help establish whether patients with suspected sensitivity to drugs of this type have enzyme abnormalities that cannot be detected by conventional laboratory techniques. Although the method discriminates between cholinesterase a...

journal_title：Clinical chemistry

authors： Faye S,Evans RT

更新日期：1986-08-01 00:00:00

abstract：:We describe a simple polarization fluoroimmunoassay for the detection of several commonly abused drugs in urine. The single reagent used, prepared by mixing four different antisera and three fluorescein-labeled derivatives, produces a polarization signal that is the average of the individual signals of the derivatives...

journal_title：Clinical chemistry

authors： Colbert DL,Childerstone M

更新日期：1987-10-01 00:00:00