Multiple drugs of abuse in urine detected with a single reagent and fluorescence polarization.

Abstract:

:We describe a simple polarization fluoroimmunoassay for the detection of several commonly abused drugs in urine. The single reagent used, prepared by mixing four different antisera and three fluorescein-labeled derivatives, produces a polarization signal that is the average of the individual signals of the derivatives. One adds urine to the pre-mixed reagent, incubates at room temperature for a few minutes, then measures fluorescence polarization. The presence of any of several abused drugs, at concentrations of 1 mg/L or more, noticeably decreases the average signal. Although other combinations are possible, the present assay detects the presence of a cocaine metabolite, amphetamine, and (or) several barbiturates.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Colbert DL,Childerstone M

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

1921-3

issue

10

eissn

0009-9147

issn

1530-8561

journal_volume

33

pub_type

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