Identification of chromosomally integrated human herpesvirus 6 by droplet digital PCR.

abstract：:The values of apolipoproteins (apo) A-I and B were determined in a population sample of hospital outpatients with a standardized method to verify if the cutpoints calculated in a cross-sectional study in the US are usable with other populations. We also tested the apolipoproteins' ability to discriminate between healt...

journal_title：Clinical chemistry

authors： Graziani MS,Zanolla L,Righetti G,Marchetti C,Mocarelli P,Marcovina SM

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:The aim of our present study was to establish new reference intervals for thyrotropin (TSH) and thyroid hormones based on National Academy of Clinical Biochemistry (NACB) criteria and regular thyroid ultrasonography. We also assessed the effect of potentially confounding factors to modulate the limits of the...

journal_title：Clinical chemistry

authors： Kratzsch J,Fiedler GM,Leichtle A,Brügel M,Buchbinder S,Otto L,Sabri O,Matthes G,Thiery J

更新日期：2005-08-01 00:00:00

abstract：:We describe a procedure for determining amitriptyline and imipramine and their active metabolites nortriptyline and desipramine, respectively, at therapeutic concentrations in human plasma by use of liquid chromatography. The drugs are extracted at pH 10.5 into hexane/isoamyl alcohol, which is evaporated and the resid...

journal_title：Clinical chemistry

authors： Vandemark FL,Adams RF,Schmidt GJ

更新日期：1978-01-01 00:00:00

abstract：:We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measureme...

journal_title：Clinical chemistry

authors： Rautela GS,Liedtke RJ

更新日期：1978-01-01 00:00:00

abstract：BACKGROUND:Measurement of serum thyroglobulin (Tg) is used to monitor patients after treatment for differentiated thyroid carcinoma (TC). Difficulty in using Tg as a biomarker of the recurrence of TC in many patients stems from the presence of endogenous anti-Tg autoantibodies (Tg-AAbs), which can interfere with immuno...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Roberts WL,Abraham D,Hoofnagle AN,Meikle AW

更新日期：2013-06-01 00:00:00

abstract：:Intra- and interindividual components of biological variation have been determined for total thyroxin (TT4), free thyroxin (FT4), total triiodothyronine (TT3), free triiodothyronine (FT3), and thyrotropin (TSH). Calculated analytical goals (CV, %) for the precision required for optimal patient care are: TT4 less than ...

journal_title：Clinical chemistry

authors： Browning MC,Ford RP,Callaghan SJ,Fraser CG

更新日期：1986-06-01 00:00:00

abstract：:We evaluated the density-gradient ultracentrifugation method in a swinging-bucket rotor (Anal Biochem 111, 149-157, 1981) in a slightly modified version for isolation and determination of high-density lipoprotein (HDL) subfractions. We prestained the serum with Coomassie Brilliant Blue R, which did not change the hydr...

journal_title：Clinical chemistry

authors： Demacker PN,van Sommeren-Zondag DF,Stalenhoef AF,Stuyt PM,van't Laar A

更新日期：1983-04-01 00:00:00

abstract：BACKGROUND:Altered germline expression of genes may represent a powerful marker of genetic or epigenetic predisposition to cancer or other diseases. METHODS:We developed and validated a method of nonfluorescent primer extension that uses a single dideoxynucleotide and denaturing HPLC (DHPLC) to analyze the relative al...

journal_title：Clinical chemistry

authors： Aceto GM,De Lellis L,Catalano T,Veschi S,Radice P,Di Iorio A,Mariani-Costantini R,Cama A,Curia MC

更新日期：2009-09-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：:We have developed a series of photochemical silver stains that are more than 100 times as sensitive as the Coomassie Blue stains. These stains facilitate the quantitative estimation of proteins in two-dimensional gel patterns. Recently, we discovered that by using light (photodevelopment) to catalyze the reduction of ...

journal_title：Clinical chemistry

authors： Merril CR,Harrington MG

更新日期：1984-12-01 00:00:00

abstract：BACKGROUND:Small RNAs are key players in the regulation of gene expression and differentiation. However, many different classes of small RNAs (sRNAs) have been described with distinct biogenesis pathways and, as a result, with different biochemical properties. To analyze sRNAs by deep sequencing, complementary DNA synt...

journal_title：Clinical chemistry

authors： Meistertzheim M,Fehlmann T,Drews F,Pirritano M,Gasparoni G,Keller A,Simon M

更新日期：2019-12-01 00:00:00

abstract：:The basic theory of thyroxin-analog-based radioimmunoassays for free thyroxin has been extended to evaluate definitively the effects arising from residual binding of the tracer analog to serum proteins. Using experimentally determined binding constants and computer simulation techniques, we studied the effects of thyr...

journal_title：Clinical chemistry

authors： Wilkins TA,Midgley JE,Barron N

更新日期：1985-10-01 00:00:00

abstract：BACKGROUND:Measurement of plasma renin is important for the clinical assessment of hypertensive patients. The most common methods for measuring plasma renin are the plasma renin activity (PRA) assay and the renin immunoassay. The clinical application of renin inhibitor therapy has thrown into focus the differences in i...

journal_title：Clinical chemistry

authors： Campbell DJ,Nussberger J,Stowasser M,Danser AH,Morganti A,Frandsen E,Ménard J

更新日期：2009-05-01 00:00:00

abstract：:A profile (chromatographic pattern) of the neutral and acidic metabolites present in cerebrospinal fluid from newborns and infants was obtained by gas-liquid chromatography. The metabolites are those extracted with ethyl acetate and diethyl ether. They are converted to trimethylsilyl ether derivatives, and chromatogra...

journal_title：Clinical chemistry

authors： Malcolm RD,Leonards R

更新日期：1976-05-01 00:00:00

abstract：BACKGROUND:There is need for a reference method for transferrin glycoforms in serum to which routine immunologic methods for the alcohol marker carbohydrate-deficient transferrin (CDT) can be traceable. We describe an improved HPLC method for transferrin glycoforms. METHODS:Transferrin was iron-saturated by mixing the...

journal_title：Clinical chemistry

authors： Helander A,Husa A,Jeppsson JO

更新日期：2003-11-01 00:00:00

abstract：:Published estimates of the imprecision characteristics of immunoassays are often based on quantities of data that seem to be inadequate. The increasing use of imprecision profiles has not necessarily improved the situation. We describe and illustrate a method of computing 95% confidence intervals for profiles estimate...

journal_title：Clinical chemistry

authors： Sadler WA,Smith MH

更新日期：1990-07-01 00:00:00

abstract：BACKGROUND:The susceptibility of plasma lipids to oxidation is thought to be a factor contributing to atherogenic risk. Various groups have studied the in vitro oxidizability of isolated LDL and examined the effects of conventional antioxidants. The drawbacks associated with the isolation of LDL for evaluation of in vi...

journal_title：Clinical chemistry

authors： Atkin MA,Gasper A,Ullegaddi R,Powers HJ

更新日期：2005-11-01 00:00:00

abstract：:We evaluated a new kinetic assay for alpha-amylase (Phadebas IsoAmylase Test), using modified starch as the substrate and a CentrifiChem 400 centrifugal analyzer system. We determined isoamylase activities by using a selective inhibitor. Results were compared with those obtained with the chromogenic Phadebas dyed-star...

journal_title：Clinical chemistry

authors： de Rijke D,Kreutzer HJ

更新日期：1983-06-01 00:00:00

abstract：:Using the Selected Method of McKenzie and Henderson (Selected Methods Clin Chem 1983;10:59-67) as a reference method, we compared the performance of 10 commercially available methods for determination of lactate dehydrogenase (LD, EC 1.1.1.27) isoenzymes. Results were expressed as percentage of total LD activity, as d...

journal_title：Clinical chemistry

authors： Moses GC,Ross ML,Henderson AR

更新日期：1988-09-01 00:00:00

abstract：:We report the accuracy, imprecision, total analytical errors, and patient misclassification errors for cholesterol measured from capillary whole blood, venous whole blood, and venous plasma samples by five devices used in public cholesterol screening environments: Reflotron, Vision, Ektachem DT-60, QuickRead, and Lipo...

journal_title：Clinical chemistry

authors： Miller WG,McKenney JM,Conner MR,Chinchilli VM

更新日期：1993-02-01 00:00:00

abstract：:Imferon (Merrell-National), an iron-dextran complex, is widely used in patients with iron deficiency. It is present in the circulation in appreciable amounts for two to three weeks after administration and interferes with all tested colorimetric iron assays, both with and without deproteinization. The amount of the pl...

journal_title：Clinical chemistry

authors： Huisman W

更新日期：1980-04-01 00:00:00

abstract：:Solid-phase-based immunoassays have traditionally been plagued by nonspecific binding to the solid phase and by slow reaction kinetics relative to reactants that are free to diffuse in solution. We have developed two novel immunoassays in which the solid phase is generated in situ after the specific binding reaction h...

journal_title：Clinical chemistry

authors： Auditore-Hargreaves K,Houghton RL,Monji N,Priest JH,Hoffman AS,Nowinski RC

更新日期：1987-09-01 00:00:00

abstract：:It has been hypothesized over many years that the autoimmune endocrine diseases are each due to antigen-specific defects in suppressor (regulatory) T lymphocyte function. There is now increasing evidence in studies of autoimmune thyroid disease and insulin-dependent diabetes mellitus that suppressor cells are activate...

journal_title：Clinical chemistry

authors： Volpé R

更新日期：1994-11-01 00:00:00

abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...

journal_title：Clinical chemistry

authors： Gellekink H,van Oppenraaij-Emmerzaal D,van Rooij A,Struys EA,den Heijer M,Blom HJ

更新日期：2005-08-01 00:00:00

abstract：:We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tra...

journal_title：Clinical chemistry

authors： Duncan P,Gochman N,Bayse D,Burnett R,Carey N,Carter R,Fellows WD,Garber C,Kessler G,McComb R,Miller W,Nast P,Ryan W,Schaffer R,Tejeda B,Vanderlinde R,Widdowson G

更新日期：1982-02-01 00:00:00

abstract：:A "sandwich" enzyme immunoassay for ferritin in human serum is described, in which horseradish peroxidase-labeled antibody and a very sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazolin-6-sulfonate), are used. The solid phase is polypropylene test tubes treated with glutaraldehyde; immunologic reactions are devel...

journal_title：Clinical chemistry

authors： Revenant MC

更新日期：1983-04-01 00:00:00

abstract：:Quantification of ethanol by a radiative energy attenuation (REA) technique was evaluated and compared with results by gas-liquid chromatography (GLC) and by the Du Pont aca. Within-assay CVs were less than 5.5%. Between-assay CVs ranged from 1.9% to 6.0% for serum and blood controls at concentrations of 0.5, 1.0, and...

journal_title：Clinical chemistry

authors： Cary PL,Whitter PD,Johnson CA

更新日期：1984-11-01 00:00:00

abstract：:Six different procedures for radioiodination of mouse epidermal growth factor (EGF) all resulted in a heterogeneous 125I-labeled EGF preparation, as analyzed by reversed-phase HPLC. EGF preparations that had been iodinated with Chloramine T, lodogen, or lodo-beads were found mainly to consist of oxidized 125I-labeled ...

journal_title：Clinical chemistry

authors： Kienhuis CB,Heuvel JJ,Ross HA,Foekens JA,Benraad TJ

更新日期：1992-05-01 00:00:00

abstract：BACKGROUND:Aberrant DNA hypermethylation of CpG islands occurs frequently throughout the genome in human colorectal cancer (CRC). A genome-wide DNA hypermethylation analysis technique using circulating cell-free DNA (cfDNA) is attractive for the noninvasive early detection of CRC and discrimination between CRC and othe...

journal_title：Clinical chemistry

authors： Li J,Zhou X,Liu X,Ren J,Wang J,Wang W,Zheng Y,Shi X,Sun T,Li Z,Kang A,Tang F,Wen L,Fu W

更新日期：2019-07-01 00:00:00

abstract：:We adapted an inhibition assay for methotrexate, involving dihydrofolate reductase from bovine liver, for use with a discrete analyzer (the ABA-100). The analyzer was used both for dilution and a 5-min pre-incubation of the sample with NADPH--enzyme reagent, and for the assay itself. The standard curve was linear betw...

journal_title：Clinical chemistry

authors： Brown LF,Johnson GF,Witte DL,Feld RD

更新日期：1980-02-01 00:00:00