Dried plasma spot measurements of ferritin and transferrin receptor for assessing iron status.

abstract：:We describe a solid-phase radioimmunometric sandwich assay for a new tumor marker defined by a monoclonal antibody (19-9). This antibody reacts with a carbohydrate antigenic determinant (CA 19-9) found at low concentrations in sera from healthy individuals but frequently increased in sera from patients with adenocarci...

journal_title：Clinical chemistry

authors： Del Villano BC,Brennan S,Brock P,Bucher C,Liu V,McClure M,Rake B,Space S,Westrick B,Schoemaker H,Zurawski VR Jr

更新日期：1983-03-01 00:00:00

abstract：BACKGROUND:Coffee consumption has been associated with several risk factors for coronary heart disease, including increased cholesterol, increased blood pressure, and increased plasma total homocysteine (tHcy). tHcy is determined by several B-vitamins. However, reports about the association between coffee intake and B-...

journal_title：Clinical chemistry

authors： Ulvik A,Vollset SE,Hoff G,Ueland PM

更新日期：2008-09-01 00:00:00

abstract：:Various laboratories have reported endogenous digoxin-like immunoreactive factor(s) (DLIF) in blood from patients in renal failure or liver failure, from newborn infants, and from third-trimester pregnant women. Similar immunoreactivity has been detected in amniotic fluid, in cord blood, and in urine and serum from no...

journal_title：Clinical chemistry

authors： Valdes R Jr

更新日期：1985-09-01 00:00:00

abstract：:A quantitative "high-performance" liquid-chromatographic method is described for determining porphyrins in human urine. Porphyrinogens in urine are first converted to the corresponding porphyrins by oxidation with iodine. Uroporphyrin, hepatacarboxylic acid porphyrin, hexacarboxylic acid porphyrin, pentacarboxylic aci...

journal_title：Clinical chemistry

authors： Abe K,Konaka R

更新日期：1989-08-01 00:00:00

abstract：:The potential errors of the one-point calibration technique are described mathematically. I examine the theoretical causes and effects of analytical errors in absorbance and of errors in determining calibration concentration, and discuss techniques for minimizing their impact. The dependence of these errors on the cal...

journal_title：Clinical chemistry

authors： Kemp GJ

更新日期：1984-07-01 00:00:00

abstract：:Measurement of the mass concentration of serum enzymes by radioimmunoassay provides direct quantitation of specific isoenzymes and may be less subject to some of the limitations of traditional assay procedures for enzymes. We describe the development of a sensitive and specific radioimmunoassay for the muscle isoenzym...

journal_title：Clinical chemistry

authors： Van Steirteghem AC,Zweig MH,Schechter AN

更新日期：1978-03-01 00:00:00

abstract：:We describe the interlaboratory testing of a candidate Reference Method (Part I) for uric acid in serum. The method is based on the ultrasound spectrophotometric quantitation of uric acid before and after incubation with uricase. A comprehensive investigation involving 12 laboratories was organized to document the tra...

journal_title：Clinical chemistry

authors： Duncan P,Gochman N,Bayse D,Burnett R,Carey N,Carter R,Fellows WD,Garber C,Kessler G,McComb R,Miller W,Nast P,Ryan W,Schaffer R,Tejeda B,Vanderlinde R,Widdowson G

更新日期：1982-02-01 00:00:00

abstract：:This ultrafiltration technique allows determination of free drug in 50 microL of serum. We ultrafiltered sera containing the following drugs--valproic acid (and its major metabolites), phenobarbital, diazepam, indomethacin, phenytoin, furosemide, and chloramphenicol--using both the commercially available micropartitio...

journal_title：Clinical chemistry

authors： Wittfoht W,Duwe K,Kuhnz W,Nau H

更新日期：1984-06-01 00:00:00

abstract：:We describe an automated enzymatic procedure for quantifying creatine in erythrocytes. In this assay, after sample clean-up, creatine kinase (CK; EC 2.7.3.2) and pyruvate kinase (EC 2.1.7.40) are used as auxiliary enzymes; lactate dehydrogenase (EC 1.1.1.27) is used in the indicator reaction. CK is also used as the st...

journal_title：Clinical chemistry

authors： Beyer C,Alting IH

更新日期：1996-02-01 00:00:00

abstract：BACKGROUND:The B-type natriuretic peptides (BNP and N-terminal pro-BNP) are secreted by the heart and, in the case of BNP, serve to maintain circulatory homeostasis through renal and vascular actions and oppose many effects of the renin-angiotensin system. Recent evidence suggests that in patients with severe heart fai...

journal_title：Clinical chemistry

authors： Mahagamasekera PG,Ruygrok PN,Palmer SC,Richards AM,Ansell GS,Nicholls MG,Pemberton CJ,Lewis LK,Yandle TG

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:The time required for bloodstream pathogen detection, identification (ID), and antimicrobial susceptibility testing (AST) does not satisfy the acute needs of disease management. Conventional methods take up to 3 days for ID and AST. Molecular diagnostics have reduced times for ID, but their promise to suppla...

journal_title：Clinical chemistry

authors： Andini N,Hu A,Zhou L,Cogill S,Wang TH,Wittwer CT,Yang S

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND:Quantification of integrated proviral HIV DNA by repetitive-sampling Alu-HIV PCR is a candidate virological tool to monitor the HIV reservoir in patients. However, the experimental procedures and data analysis of the assay are complex and hinder its widespread use. Here, we provide an improved and simplified...

journal_title：Clinical chemistry

authors： De Spiegelaere W,Malatinkova E,Lynch L,Van Nieuwerburgh F,Messiaen P,O'Doherty U,Vandekerckhove L

更新日期：2014-06-01 00:00:00

abstract：BACKGROUND:Calcitonin (CT) is a sensitive marker for evaluation of medullary thyroid cancer (MTC). However, CT measurement can vary with assay- and nonassay-dependent factors, and procalcitonin (PCT) measurement has been proposed for evaluating questionable increases in CT. METHODS:We tested 2 fully automated CT assay...

journal_title：Clinical chemistry

authors： Kratzsch J,Petzold A,Raue F,Reinhardt W,Bröcker-Preuss M,Görges R,Mann K,Karges W,Morgenthaler N,Luster M,Reiners C,Thiery J,Dralle H,Fuhrer D

更新日期：2011-03-01 00:00:00

abstract：:We describe a gas-liquid chromatographic method for determining mephenytoin and its active metabolite, desmethylmephenytoin, in human serum. 5-Methyl-5-phenylhydantoin is used as the internal standard. The method involves extraction of the drugs by adsorption onto charcoal and off-column derivatization to their pentyl...

journal_title：Clinical chemistry

authors： Raisys VA,Zebelman AM,MacMillan SF

更新日期：1979-01-01 00:00:00

abstract：:We find lag phases exceeding 20 min in measuring creatine kinase activity, by using the kinetic creatin phosphate leads to creatine assay, in sera of some patients with carcinoma metastatic to the liver. Such long lag phases are accompanied by a decreased apparent enzyme activity. These problems are eliminated by addi...

journal_title：Clinical chemistry

authors： Bruns DE,Morgan WS,Davis JE,Ladenson JH

更新日期：1976-11-01 00:00:00

abstract：:This case demonstrates the importance of monitoring theophylline concentrations in serum during therapy with this drug. The commonly used body-weight-derived guidelines for therapeutic dose are not adequate and do not preclude the necessity for such monitoring. The ultraviolet spectrophotometric methods available for ...

journal_title：Clinical chemistry

authors：

更新日期：1978-09-01 00:00:00

abstract：:We present a unified approach to the use of linear models and matrix least squares with the intention of providing a better understanding of the techniques themselves and of the statistics that arise from these techniques as they are used in clinical chemistry. Emphasis is placed on the importance of appropriate exper...

journal_title：Clinical chemistry

authors： Deming SN,Morgan SL

更新日期：1979-06-01 00:00:00

abstract：:This two-site immunoradiometric assay for human parathyrin-related protein 1-86 (PTHRP1-86) in plasma uses a mouse monoclonal antibody to PTHRP1-34 coupled to cellulose particles for immunoextraction of N-terminal immunoreactivity, and a rabbit antiserum to PTHRP37-67 that is indirectly labeled with 125I-labeled PTHRP...

journal_title：Clinical chemistry

authors： Ratcliffe WA,Norbury S,Heath DA,Ratcliffe JG

更新日期：1991-05-01 00:00:00

abstract：BACKGROUND:Direct equilibrium dialysis and direct ultrafiltration free thyroxine (T(4)) assays rely on semipermeable membranes to exclude T(4)-binding serum proteins from dialysates and ultrafiltrates. The presence of these proteins in dialysates or ultrafiltrates will yield spuriously high free T(4) values when free T...

journal_title：Clinical chemistry

authors： Fritz KS,Wilcox RB,Nelson JC

更新日期：2007-05-01 00:00:00

abstract：:We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamina...

journal_title：Clinical chemistry

authors： Nicholson JR,Savory MG,Savory J,Wills MR

更新日期：1989-03-01 00:00:00

abstract：:We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%. The assay is simple, rapid, and inexpensive. Albumin excretion was 6.2 (SD 4.1) mg/...

journal_title：Clinical chemistry

authors： Krämer BK,Jesse U,Ress KM,Schmülling RM,Risler T

更新日期：1987-04-01 00:00:00

abstract：:In this method, leukocytes were isolated from 6 mL of EDTA-blood by density-gradient centrifugation and subsequently incubated with rhodamine isothiocyanate (RITC)-conjugated high-density lipoproteins (HDL). The receptor-bound conjugate particles were determined by fluorescent flow cytometry and compared with 125I-lab...

journal_title：Clinical chemistry

authors： Schmitz G,Wulf G,Brüning T,Assmann G

更新日期：1987-12-01 00:00:00

abstract：:A competitive solid-phase fluoroenzymeimmunoassay has been developed for rapidly measuring 5 beta-pregnane-3 alpha, 20 alpha-diol-3 alpha-glucuronide (Pd-3G) directly in diluted specimens of pre-breakfast urine. The assay involves use of an antiserum to Pd-3G and enzyme-labeled antigen prepared by chemically linking g...

journal_title：Clinical chemistry

authors： Shah H,Saranko AM,Härkönen M,Adlercreutz H

更新日期：1984-02-01 00:00:00

abstract：:We describe a mass-fragmentographic method for determination in urine of the following metabolites of L-3,4-dihydroxyphenylalanine and dopamine: vanillactic acid, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylethanol, and 3,4-dihydroxyphenylethanol. Deuterated analogs were used as internal standards. The me...

journal_title：Clinical chemistry

authors： Muskiet FA,Fremouw-Ottevangers DC,van der Meulen J,Wolthers BG,de Vries JA

更新日期：1978-01-01 00:00:00

abstract：BACKGROUND:Hyperhomocysteinemia (HHcy) has been linked to impaired left ventricular function and clinical class in patients with chronic heart failure. We hypothesized that HHcy stimulates myocardial brain natriuretic peptide (BNP) expression and induces adverse left ventricular remodeling. METHODS:We randomized 50 ra...

journal_title：Clinical chemistry

authors： Herrmann M,Taban-Shoma O,Hübner U,Pexa A,Kilter H,Umanskaya N,Straub RH,Böhm M,Herrmann W

更新日期：2007-04-01 00:00:00

abstract：:The molar absorptivity of unconjugated bilirubin in caffeine reagent is independent of the protein matrix. This finding, together with the simplicity of a dilution step for direct spectrophotometry, will improve the calibration methods of bilirubin and make them more nearly accurate. This is encouraging for the develo...

journal_title：Clinical chemistry

authors： Vink KL,Schuurman W,van Gansewinkel R

更新日期：1986-07-01 00:00:00

abstract：BACKGROUND:The discovery of cell-free fetal DNA in maternal plasma has opened up new possibilities for noninvasive prenatal diagnosis and monitoring. Among the fetal markers that have been described, methylation markers are sex and polymorphism independent. Methylation-sensitive restriction endonucleases are commonly u...

journal_title：Clinical chemistry

authors： Tong YK,Chiu RW,Leung TY,Ding C,Lau TK,Leung TN,Lo YM

更新日期：2007-11-01 00:00:00

abstract：BACKGROUND:Tacrolimus is metabolized predominantly to 13-O-demethyltacrolimus in the liver and intestine by cytochrome P450 3A (CYP3A). Patients with high concentrations of CYP3A5, a CYP3A isoenzyme polymorphically produced in these organs, require higher doses of tacrolimus, but the exact mechanism of this association...

journal_title：Clinical chemistry

authors： Kamdem LK,Streit F,Zanger UM,Brockmöller J,Oellerich M,Armstrong VW,Wojnowski L

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Fecal calprotectin (FC) has been proposed as a marker of inflammatory bowel disease (IBD), but few studies have evaluated its usefulness in patients with chronic diarrhea of various causes. We evaluated the diagnostic accuracy of a FC assay in identifying "organic" causes of chronic diarrhea in consecutive a...

journal_title：Clinical chemistry

authors： Carroccio A,Iacono G,Cottone M,Di Prima L,Cartabellotta F,Cavataio F,Scalici C,Montalto G,Di Fede G,Rini G,Notarbartolo A,Averna MR

更新日期：2003-06-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal testing (NIPT) uses cell-free DNA (cfDNA) as an analyte to detect copy-number alterations in the fetal genome. Because maternal and fetal cfDNA contributions are comingled, changes in the maternal genome can manifest as abnormal NIPT results. Circulating tumor DNA (ctDNA) present in case...

journal_title：Clinical chemistry

authors： Dharajiya NG,Grosu DS,Farkas DH,McCullough RM,Almasri E,Sun Y,Kim SK,Jensen TJ,Saldivar JS,Topol EJ,van den Boom D,Ehrich M

更新日期：2018-02-01 00:00:00