Abstract:
BACKGROUND:The B-type natriuretic peptides (BNP and N-terminal pro-BNP) are secreted by the heart and, in the case of BNP, serve to maintain circulatory homeostasis through renal and vascular actions and oppose many effects of the renin-angiotensin system. Recent evidence suggests that in patients with severe heart failure, circulating immunoreactive BNP is made up mainly of metabolites that may have reduced bioactivity. We hypothesized that BNP may be degraded before it even leaves the heart. METHODS:Peripheral venous plasma plus atrial and ventricular tissue, obtained from explanted hearts at the time of transplantation, were collected from 3 patients with end-stage heart failure. In a separate study, plasma was collected from the coronary sinus and femoral artery of 3 separate patients undergoing cardiac catheterization. Plasma C18 reverse-phase extracts were separated on reverse-phase HPLC, and the collected fractions were subjected to RIAs with highly specific antisera directed to the amino- and carboxy-terminal ends of BNP(1-32). RESULTS:ProBNP, BNP(1-32), and 2 major BNP metabolites were present in atrial and ventricular tissue, where BNP(1-32) represented 45% and 70% of total processed BNP, respectively. Neither BNP(1-32) nor the 2 metabolites were detected in peripheral venous plasma. Nor was BNP(1-32) detected in matching coronary sinus and femoral artery plasma from the 3 patients undergoing cardiac catheterization. CONCLUSIONS:BNP(1-32) is partly degraded within the hearts of patients with end-stage heart failure, and even in patients with relatively well-preserved left ventricular systolic function, only BNP metabolites enter the systemic circulation.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Mahagamasekera PG,Ruygrok PN,Palmer SC,Richards AM,Ansell GS,Nicholls MG,Pemberton CJ,Lewis LK,Yandle TGdoi
10.1373/clinchem.2013.210435subject
Has Abstractpub_date
2014-03-01 00:00:00pages
549-58issue
3eissn
0009-9147issn
1530-8561pii
clinchem.2013.210435journal_volume
60pub_type
杂志文章abstract::We have modified an automated measurement system of urinary iodine (UI) and established a sensitive UI assay system by using ultraviolet (UV) digestion. The automated system is sensitive enough to detect concentrations of UI < 0.78 mumol/L (< 10 micrograms/dL) in a small volume of urine (500 microL). Sample throughput...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:2002-09-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1995-07-01 00:00:00
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
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doi:
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