Abstract:
:We used ion-exchange column chromatography and electrophoresis on polyacrylamide gel to compare the acid phosphatase isoenzymes of prostate and leukocytes. The major isoenzyme of the prostate is band 2A; only a trace of band 2B was observed. However, the major isoenzymes of leukocytes are band 4 and band 2B, and only a small amount of band 2A was observed. The three isoenzymes isolated from leukocytes or prostate gland react to the antiserum prepared against the aicd phosphatase isoenzyme of seminal fluid. Acid phosphatases of leukocytes other than the three isoenzymes mentioned above did not interact with the antiserum.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Lam WK,Yam LT,Wilbur HJ,Taft E,Li CYsubject
Has Abstractpub_date
1979-07-01 00:00:00pages
1285-9issue
7eissn
0009-9147issn
1530-8561journal_volume
25pub_type
杂志文章abstract::We report the presence of complexes between aspartate aminotransferase (AST, EC 2.6.1.1) and immunoglobulin (Ig) in the serum of a patient suffering from lung cancer with metastasis to the liver. After fractionation of the serum by gel filtration, AST-Ig complexes (AST-IgA, AST-IgG) were demonstrated by counterimmunoe...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the feasibility of infrared (IR) spectroscopy for the simultaneous quantification of serum LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) concentrations. METHODS:Serum samples (n = 90) were obtained. Duplicate aliquots (5 microL) of the serum specimens were dried...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::We examined the comparative behavior of subject-specific multivariate and univariate reference regions, using both computer-generated data and serial (semi-annual) measurements of selected analytes in subjects from a large health-maintenance program. Univariate studies under both homeostatic and random-walk time-serie...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::We describe a case of sulfhemoglobinemia associated with toxic paint ingestion. Blood gases, oxygen content, and fractional hemoglobin derivatives were assayed with Radiometer 520 and OSM3 instruments. Although the CO-oximeters indicated the presence of sulfhemoglobin (SulfHb), the results were not quantitative. An OS...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:An accelerated stability model based on the Arrhenius Equation can be used to estimate stability of diagnostic reagents. Here we review 3 examples in which the model does not accurately predict the stability of diagnostic reagents. METHODS:We prepared several pilot lots of quality controls materials contain...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1093/clinchem/hvaa282
更新日期:2020-12-04 00:00:00
abstract::To understand the regulation of immunity one must understand the processes and the consequences of the activation of the lymphoid cell, its clonal proliferation, and the ultimate differentiation of the progeny into the effector elements that constitute the immune system. By use of polyclonal mitogens, individual T-cel...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::An enzymatic, fluorometric method is described for determination of serum urea on silicone-rubber pads. In this method, the reagents are lyophilized on the surface of the pads, NADH on one side and a mixture of urease, glutamate dehydrogenase, and alpha-ketoglutarate on the other. The rate of disappearance of NADH flu...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract:BACKGROUND:The recent development of nonradioactive automated assays for serum parathyroid hormone (PTH) and 25-hydroxyvitamin D (25OHD) has made measurement of these two hormones possible in many laboratories. In this study, we compared two new assays for PTH and 25OHD adapted on an automated analyzer, the LIAISON, wi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.037606
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND:A previously developed monoclonal/polyclonal ELISA (Mono/Poly) to detect plasma concentrations of osteopontin (OPN) was shown to provide prognostic information in breast, prostate, and other cancers. Here we describe the clinical validation of a new dual monoclonal (Dual Mono) assay. We compared both assays ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2008.117465
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Low concentrations of cardiac troponin (cTn) have been recommended for rapid rule-out of acute myocardial infarction (AMI). We examined the Beckman Coulter Access high-sensitivity cardiac troponin I (hs-cTnI) assay to identify a single test threshold that can safely rule out AMI. METHODS:This analysis used ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2017.283887
更新日期:2018-05-01 00:00:00
abstract::The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on t...
journal_title:Clinical chemistry
pub_type: 历史文章,杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::The IFCC-recommended procedure for aspartate aminotransferase (EC 2.6.1.1) was adapted to the centrifugal analyzer and evaluated during five years. The main hindrance to widespread use of the recommended method is the need for pre-incubation with pyridoxal phosphate. The present method includes a 10-min pre-incubation...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Concentrations of triglycerides are increased and concentrations of high-density lipoprotein (HDL) cholesterol are low in women with hyperandrogenism. These alterations could be related to excessive androgen or estrogen, to hyperinsulinism, or to a combination of these abnormalities. We examined their independent infl...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:The time required for bloodstream pathogen detection, identification (ID), and antimicrobial susceptibility testing (AST) does not satisfy the acute needs of disease management. Conventional methods take up to 3 days for ID and AST. Molecular diagnostics have reduced times for ID, but their promise to suppla...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2018.290189
更新日期:2018-10-01 00:00:00
abstract::In this enzyme-linked immunoassay for human thyrotropin (TSH) in unextracted serum we use 96-well immunoenzymometric assay plates, first coated with polyclonal antibody to TSH, then incubated with the serum samples and reacted with mouse monoclonal antibody to human TSH. After incubation with alkaline phosphatase-labe...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-07-01 00:00:00
abstract::The commonly used methods of assessing the precision of the automated leukocyte differential have certain drawbacks that affect the validity and comparability of results. In the present report, we introduce a procedure based on building precision profiles from a large number of within-run imprecision experiments. The ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::This enzyme immunoassay detects adenosine deaminase binding protein (ABP), a glycoprotein that is shed from the brush border of the proximal tubule in kidney damage. Two monoclonal antibodies, URO-4 and URO-4a, each react with different epitopes on ABP and are used as the "sandwich" pair of antibodies. A linear standa...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract:BACKGROUND:Plasma concentrations of small dense (sd)-LDL are associated with the prevalence of cardiovascular events. However, the special equipment and long assay times required for sd-LDL measurement have hindered its clinical application. Herein, we report development of a simple homogeneous assay for sd-LDL-cholest...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2010.149559
更新日期:2011-01-01 00:00:00
abstract::A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of rete...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract:BACKGROUND:Mitochondrial diseases are clinically and genetically heterogeneous, with variable penetrance, expressivity, and differing age of onset. Disease-causing point mutations and large deletions in the mitochondrial genome often exist in a heteroplasmic state. Current molecular analyses require multiple different ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2011.181438
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND:A reduced response to aspirin and clopidogrel predicts ischemic events, but reliable tests are needed to identify low responders. We compared 3 platelet-function tests during long-term dual treatment with aspirin and clopidogrel. METHODS:Patients who underwent a percutaneous coronary intervention and were r...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2009.137471
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS:We evaluated the sensitivity and specificity of homogeneous scanning, using a saturatin...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.029751
更新日期:2004-10-01 00:00:00
abstract::A three-parameter model for directly estimating imprecision profiles from replicated immunoassay results is compared with a six-parameter indirect profile model obtained by the usual method of combining error in the raw response measurements with the slope of the standard curve. Direct estimation is likely to be less ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-06-01 00:00:00
abstract::We describe a sensitive, simple, and rapid two-site immunoenzymometric assay for thyrotropin in dried blood samples on filter paper, for use in screening for neonatal primary hypothyroidism. In this method, two dried-blood spots of 3 mm diameter (equivalent to about 5.4 microliter of blood) are incubated overnight wit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::The human ras gene plays a fundamental role in the transduction of extracellular signals to the nucleus, thereby regulating cell growth and differentiation. Point mutations in the ras gene convert it into a transforming oncogene that has been found in many solid and hematologic malignancies. We describe a rapid and se...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::The metabolism of the synthetic substrate 7-ethoxyresorufin is a selective measure of the activity of cytochrome P-448 monooxygenase, the subset of cytochrome P-450-mediated enzymes preferentially induced by polycyclic aromatic hydrocarbons and related compounds. 7-Ethoxycoumarin metabolism, on the other hand, reflect...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract:BACKGROUND:Variability among assays used to measure intact parathyroid hormone (iPTH) is of particular concern because of the routine use of iPTH assay results to guide management of osteodystrophy and calcium metabolism in patients with end-stage renal disease (ESRD). The aim of this study was to determine the extent ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.071589
更新日期:2006-09-01 00:00:00
abstract::We used a previously reported agarose-gel electrophoresis system to study the lipoprotein patterns in patients with type 3 hyperlipoproteinemia. All samples from 20 subjects tested revealed the presence of two bands with beta-lipoprotein mobility, irrespective of quantitative changes in plasma lipids induced by diet o...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::We have developed a series of photochemical silver stains that are more than 100 times as sensitive as the Coomassie Blue stains. These stains facilitate the quantitative estimation of proteins in two-dimensional gel patterns. Recently, we discovered that by using light (photodevelopment) to catalyze the reduction of ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-12-01 00:00:00
abstract::We describe optimal conditions for immobilization of two monoclonal antibodies to progesterone for solid-phase assays. Polystyrene surfaces are refined with Protein A to achieve uniform, reproducible, stable, and sterically accessible immobilization of immunoglobulins (IgG). To this end, we optimized the amount of imm...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-08-01 00:00:00