Abstract:
:We describe a chromatographic procedure for sensitive (2.0 mumol/L), specific quantification of vanillylmandelic acid (VMA) in urine with iso-VMA as an internal standard. After rapid extraction from urine, the VMA is determined by isocratic reversed-phase ion-pair chromatography on a bonded C8 column and detection of the native fluorescence on excitation at 285 nm. The fluorescence signal is quite dependent on the pH of the mobile phase. Results by the method vary linearly with VMA concentration up to 320 mumol/L and correlate well (r = 0.9880) with those obtained by conventional ultraviolet spectrophotometry. The mean 24-h excretion of VMA from 29 healthy volunteers was 21.4 (SD 5.4) mumol.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Lindgren K,Rodopoulos Nsubject
Has Abstractpub_date
1986-04-01 00:00:00pages
693-6issue
4eissn
0009-9147issn
1530-8561journal_volume
32pub_type
杂志文章abstract::This simple polarization fluoroimmunoassay for detection of amphetamine in urine involves the use of a single reagent: suitably diluted antiserum plus fluorescein-labeled amphetamine as tracer. To this, one adds 10 microL of urine, incubates for a few minutes at room temperature, and measures the fluorescence polariza...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
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更新日期:1976-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
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更新日期:1988-01-01 00:00:00
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doi:
更新日期:1990-11-01 00:00:00
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更新日期:1981-06-01 00:00:00
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更新日期:1990-06-01 00:00:00
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