Increases of cardiac troponin in conditions other than acute coronary syndrome and heart failure.

abstract：:Circadian variations of alanine aminopeptidase (EC 3.4.11.2), gamma-glutamyltransferase (EC 2.3.2.2), and N-acetyl-beta-glucosaminidase (EC 3.2.1.30) in urine were studied in 10 healthy children, ages six to 15 years. Urine specimens were collected during 24 h, grouped into four time intervals. Enzymes were measured s...

journal_title：Clinical chemistry

authors： Feldmann D,Flandrois C,Jardel A,Phan TM,Aymard P

更新日期：1989-05-01 00:00:00

abstract：:We compared the analytical performance of the Kodak Ektachem XR700 assays of iron (Fe) and total iron-binding capacity (TIBC) with that of a conventional ferrozine assay (performed with a Cobas-Bio) and occasionally with that of atomic-absorption spectrometry (AAS). The correlation was modest between Kodak and Cobas-B...

journal_title：Clinical chemistry

authors： Vercammen M,Goedhuys W,Boeyckens A,De Roy R,Sennesael J,Sevens C,Gorus F

更新日期：1990-10-01 00:00:00

abstract：:This communication describes and evaluates an improved routine methodology for quantitating clinical proteinuria. Based on investigations of Piscator and of Savory et al., a modified Tsuchiya's reagent (ethanolic HCI-phosphotungstic acid) is used to precipitate proteins at 56 degrees C, followed by biuret spectrophoto...

journal_title：Clinical chemistry

authors： Rice EW

更新日期：1975-03-01 00:00:00

abstract：:Determination of zinc in blood plasma by flameless atomic absorption spectrometry is discussed, with particular reference to the protocol required for the successful use of the Varian-Techtron Carbon Rod Atomizer. Cup aging is shown to be an important factor in limiting the precision of this analytical technique and ...

journal_title：Clinical chemistry

authors： Chooi MK,Todd JK,Boyd ND

更新日期：1975-04-01 00:00:00

abstract：BACKGROUND:KLK5 is a newly discovered human kallikrein gene. Many kallikrein genes have been found to be differentially expressed in various malignancies, and prostate-specific antigen (PSA; encoded by the KLK3 gene) is the best tumor marker for prostate cancer. Like the genes that encode PSA and other kallikreins, the...

journal_title：Clinical chemistry

authors： Yousef GM,Scorilas A,Kyriakopoulou LG,Rendl L,Diamandis M,Ponzone R,Biglia N,Giai M,Roagna R,Sismondi P,Diamandis EP

更新日期：2002-08-01 00:00:00

abstract：BACKGROUND:Adiponectin is an adipocyte-derived hormone circulating in different multimer complexes. The high-molecular-weight (HMW) complex is likely the active form of this protein and has been recognized as a risk marker for type 2 diabetes and coronary artery disease (CAD). Because quantification of HMW adiponectin ...

journal_title：Clinical chemistry

authors： Liu D,Schuster T,Baumann M,Roos M,Sollinger D,Lutz J,Heemann U,von Eynatten M

更新日期：2009-03-01 00:00:00

abstract：BACKGROUND:Test repetition could be a readily modifiable component of laboratory utilization. Laboratory test repetition has not been rigorously studied at a population-based level. Our objective was to determine the prevalence of, and charges associated with, repetition of eight common laboratory tests. METHODS:We pe...

journal_title：Clinical chemistry

authors： van Walraven C,Raymond M

更新日期：2003-12-01 00:00:00

abstract：BACKGROUND:5α-Reductase 2 deficiency (5ARD) is a known cause of 46,XY disorders of sex development (DSD). Traditionally, the diagnosis relies on dihydrotestosterone (DHT) measurement, but the results are often equivocal, potentially leading to misdiagnosis. We reviewed alternative approaches for diagnosis of 5ARD. MET...

journal_title：Clinical chemistry

authors： Chan AO,But BW,Lee CY,Lam YY,Ng KL,Tung JY,Kwan EY,Chan YK,Tsui TK,Lam AL,Tse WY,Cheung PT,Shek CC

更新日期：2013-05-01 00:00:00

abstract：:A competitive solid-phase fluoroenzymeimmunoassay has been developed for rapidly measuring 5 beta-pregnane-3 alpha, 20 alpha-diol-3 alpha-glucuronide (Pd-3G) directly in diluted specimens of pre-breakfast urine. The assay involves use of an antiserum to Pd-3G and enzyme-labeled antigen prepared by chemically linking g...

journal_title：Clinical chemistry

authors： Shah H,Saranko AM,Härkönen M,Adlercreutz H

更新日期：1984-02-01 00:00:00

abstract：BACKGROUND:FMR1 full mutations (FMs) (CGG expansion >200) in males mosaic for a normal (<45 CGG) or gray-zone (GZ) (45-54 CGG) allele can be missed with the standard 2-step fragile X syndrome (FXS) testing protocols, largely because the first-line PCR tests showing a normal or GZ allele are not reflexed to the second-l...

journal_title：Clinical chemistry

authors： Aliaga SM,Slater HR,Francis D,Du Sart D,Li X,Amor DJ,Alliende AM,Santa Maria L,Faundes V,Morales P,Trigo C,Salas I,Curotto B,Godler DE

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:The prognostic value of changes in paraprotein markers after stem cell transplantation is unknown. We evaluated disease response using serum immunofixation (s-IFIX), total kappa and lambda ratio (KLR), and free light chain (FLC) ratio in myeloma patients who underwent autologous or autologous plus allogeneic...

journal_title：Clinical chemistry

authors： Giarin MM,Giaccone L,Sorasio R,Sfiligoi C,Amoroso B,Cavallo F,Cipriani A,Palumbo A,Boccadoro M

更新日期：2009-08-01 00:00:00

abstract：:We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this me...

journal_title：Clinical chemistry

authors： Shihabi ZK,Oles KS

更新日期：1994-10-01 00:00:00

abstract：:We measured changes in concentrations of magnesium, zinc, calcium, potassium, cholesterol [total and high-density lipoproteins (HDL)], total creatine kinase (CK), and CK isoenzyme-MB in plasma (PI) and/or erythrocytes (Erc) from apparently healthy subjects and from patients with either pre-infarction syndrome (PIS) or...

journal_title：Clinical chemistry

authors： Speich M,Gelot S,Arnaud P,Nicolas G

更新日期：1988-10-01 00:00:00

abstract：BACKGROUND:Tacrolimus is metabolized predominantly to 13-O-demethyltacrolimus in the liver and intestine by cytochrome P450 3A (CYP3A). Patients with high concentrations of CYP3A5, a CYP3A isoenzyme polymorphically produced in these organs, require higher doses of tacrolimus, but the exact mechanism of this association...

journal_title：Clinical chemistry

authors： Kamdem LK,Streit F,Zanger UM,Brockmöller J,Oellerich M,Armstrong VW,Wojnowski L

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Growth differentiation factor 15 (GDF15), a stress-responsive cytokine produced in cardiovascular cells under conditions of inflammation and oxidative stress, is emerging as an important prognostic marker in individuals with and without existing cardiovascular disease (CVD). We therefore examined the clinica...

journal_title：Clinical chemistry

authors： Ho JE,Mahajan A,Chen MH,Larson MG,McCabe EL,Ghorbani A,Cheng S,Johnson AD,Lindgren CM,Kempf T,Lind L,Ingelsson E,Vasan RS,Januzzi J,Wollert KC,Morris AP,Wang TJ

更新日期：2012-11-01 00:00:00

abstract：:Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to form active beta-galactosidase. Using recombinant D...

journal_title：Clinical chemistry

authors： Henderson DR,Friedman SB,Harris JD,Manning WB,Zoccoli MA

更新日期：1986-09-01 00:00:00

abstract：BACKGROUND:Formalin-fixed paraffin-embedded (FFPE) tumor material represents a valuable resource for the analysis of RNA-based biomarkers, both in research laboratories and in routine clinical testing. A robust and automated RNA-extraction method with a high sample throughput is required. METHODS:We evaluated extracti...

journal_title：Clinical chemistry

authors： Bohmann K,Hennig G,Rogel U,Poremba C,Mueller BM,Fritz P,Stoerkel S,Schaefer KL

更新日期：2009-09-01 00:00:00

abstract：:We present a simple method for subclass typing of IgG paraproteins, with which we have demonstrated a large number of paraproteins that were undetected by conventional immunofixation techniques. The types and distribution of IgG subclass paraproteins were analyzed in 92 human sera in which IgG paraproteins had been de...

journal_title：Clinical chemistry

authors： Klouche M,Bradwell AR,Wilhelm D,Kirchner H

更新日期：1995-10-01 00:00:00

abstract：BACKGROUND:Evidence suggests that systemic inflammation may adversely impact HDL function. In this study we sought to evaluate the independent and incremental predictive performance of GlycA-a novel serum inflammatory biomarker that is an aggregate measure of enzymatically glycosylated acute phase proteins-and HDL subc...

journal_title：Clinical chemistry

authors： McGarrah RW,Kelly JP,Craig DM,Haynes C,Jessee RC,Huffman KM,Kraus WE,Shah SH

更新日期：2017-01-01 00:00:00

abstract：:For 51 liver allograft recipients, we evaluated whether serum profiles of delta (Bd) and conjugated bilirubins (Bc) could be used to diagnose rejection during the first 30-50 postoperative days, in comparison with histology as the "gold standard." Daily measurements of aspartate aminotransferase, alkaline phosphatase,...

journal_title：Clinical chemistry

authors： Wu TW,Levy GA,Yiu S,Au JX,Greig PD,Strasberg SM,Ettles M,Abecassis M,Superina RA,Langer B

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS:After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleo...

journal_title：Clinical chemistry

authors： Bullock GC,Bruns DE,Haverstick DM

更新日期：2002-12-01 00:00:00

abstract：BACKGROUND:External quality assurance (EQA) programs for diagnostic tests based on nucleic acid amplification have not been widely implemented in clinical laboratories and remain limited to few tests. Development of specific EQA programs based on application-based proficiency testing for any diagnostic molecular target...

journal_title：Clinical chemistry

authors： Raggi CC,Pinzani P,Paradiso A,Pazzagli M,Orlando C

更新日期：2003-05-01 00:00:00

abstract：:Ornithine carbamoyltransferase (EC 2.1.3.3) activity is a sensitive, specific indicator of hepatocellular injury. This paper describes development of an improved automated procedure for measurement of this activity. Triethanolamine-ethylenediaminetetraacetate is used as a buffer, and activity is determined by measurin...

journal_title：Clinical chemistry

authors： Clayson KJ,Fine JS,Strandjord PE

更新日期：1975-05-01 00:00:00

abstract：:The most popular pretreatment method of plasma samples for the measurement of ascorbate (AsA) and dehydroascorbate (DHA) has been an acidic deproteinization via metaphosphoric acid or trichloroacetic acid. In general, DHA is absent in plasma samples prepared from human blood in a conventional manner. However, when the...

journal_title：Clinical chemistry

authors： Koshiishi I,Mamura Y,Liu J,Imanari T

更新日期：1998-04-01 00:00:00

abstract：BACKGROUND:The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes. METHODS:Re...

journal_title：Clinical chemistry

authors： Ståhlberg A,Thomsen C,Ruff D,Åman P

更新日期：2012-12-01 00:00:00

abstract：:We measured the concentration of erythrocyte protoporphyrin in 43 blood samples by two fluorometric methods. In the indirect method, protoporphyrin was extracted from blood and the fluorescence of the free acid in the extract was determined with a filter-fluorometer. These results were compared with results of direct ...

journal_title：Clinical chemistry

authors： Peter F,Growcock G,Strunc G

更新日期：1978-09-01 00:00:00

abstract：:o-Phthaldialdehdye reagent was used in assaying urea with a miniature centrifugal analyzer. Seventeen samples can be assayed kinetically in 2 min at a current cost of less than a penny per test. The reaction is initiated in the instrument upon simultaneous mixing of the o-phthaldialdehyde reagent containing 10 microli...

journal_title：Clinical chemistry

authors： Levinson SS

更新日期：1978-12-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：:In this new method for determining urinary protein, the reaction is complete within 10 min at 37 degrees C. This method is applicable to automated as well as manual measurements. Protein concentration and absorbance at 600 nm are linearly related throughout a wide range of concentrations, 10 to 16 000 mg/L. However, t...

journal_title：Clinical chemistry

authors： Watanabe N,Kamei S,Ohkubo A,Yamanaka M,Ohsawa S,Makino K,Tokuda K

更新日期：1986-08-01 00:00:00

abstract：BACKGROUND:Little is known about trace metal alterations in the bones of dialysis patients or whether particular types of renal osteodystrophy are associated with either increased or decreased skeletal concentrations of trace elements. Because these patients are at risk for alterations of trace elements as well as for ...

journal_title：Clinical chemistry

authors： D'Haese PC,Couttenye MM,Lamberts LV,Elseviers MM,Goodman WG,Schrooten I,Cabrera WE,De Broe ME

更新日期：1999-09-01 00:00:00