Aluminum, iron, lead, cadmium, copper, zinc, chromium, magnesium, strontium, and calcium content in bone of end-stage renal failure patients.

abstract：:I describe a simple, single-tube batch fractionation procedure for separating MM and MB isoenzymes of creatine kinase on a macroporous strong anion exchanger (AG MP-1, Bio-Rad Laboratories). The isoenzymes can be separated in less than 3 min, with a resulting dilution of the serum with no more than an equal volume of ...

journal_title：Clinical chemistry

authors： Morin LG

更新日期：1976-01-01 00:00:00

abstract：BACKGROUND:Reduced renal function is an established risk factor for cardiovascular events. We compared 3 measures of renal function--serum cystatin C, serum creatinine, and calculated creatinine clearance--as predictors of subsequent cardiovascular events in a community-based population of elderly individuals. METHODS...

journal_title：Clinical chemistry

authors： Beilby J,Divitini ML,Knuiman MW,Rossi E,Hung J

更新日期：2010-05-01 00:00:00

abstract：:We modified a one-step, two-site, double monoclonal immunoenzymometric assay (Abbott Laboratories) for serum alpha1-fetoprotein (AFP) to increase its sensitivity and improve test precision at the low end. We increased sample size, incubation interval, and reaction time and temperature, and decreased the final reaction...

journal_title：Clinical chemistry

authors： Shahangian S,Fritsche HA Jr,Hughes JI

更新日期：1987-04-01 00:00:00

abstract：:This relatively simple, rapid method for quantification of homovanillic acid (HVA) and vanillylmandelic acid (VMA) involves solvent extraction and capillary gas chromatography. With use of this extraction method, the overall analytical recovery from an aqueous solution is 92.5% for HVA and 79.2% for VMA. 3,4-Dihydroxy...

journal_title：Clinical chemistry

authors： Tuchman M,Crippin PJ,Krivit W

更新日期：1983-05-01 00:00:00

abstract：:To understand the regulation of immunity one must understand the processes and the consequences of the activation of the lymphoid cell, its clonal proliferation, and the ultimate differentiation of the progeny into the effector elements that constitute the immune system. By use of polyclonal mitogens, individual T-cel...

journal_title：Clinical chemistry

authors： Kettman J,Lefkovits I

更新日期：1984-12-01 00:00:00

abstract：:We combined the amplification refractory mutation system (ARMS) and fluorescence polarization (FP) to give a homogeneous genomic DNA genotype analysis method. Oligonucleotide probes labeled with the fluorescein dyes fluorescein isothiocyanate and 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein and the rhodamine dye 6-c...

journal_title：Clinical chemistry

authors： Gibson NJ,Gillard HL,Whitcombe D,Ferrie RM,Newton CR,Little S

更新日期：1997-08-01 00:00:00

abstract：BACKGROUND:A simple point-of-care method for measuring leukocyte counts in a doctor's office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils ...

journal_title：Clinical chemistry

authors： Rundström G,Jonsson A,Mårtensson O,Mendel-Hartvig I,Venge P

更新日期：2007-02-01 00:00:00

abstract：BACKGROUND:Plant-derived cardenolides reportedly possess anticancer properties in human leukemic cells via selective induction of apoptosis, cell cycle arrest, and differentiation. Selective induction of apoptosis with mammalian-derived digoxin-like immunoreactive factor (DLIF) could provide new strategies for anticanc...

journal_title：Clinical chemistry

authors： Ihenetu K,Qazzaz HM,Crespo F,Fernandez-Botran R,Valdes R Jr

更新日期：2007-07-01 00:00:00

abstract：:A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint...

journal_title：Clinical chemistry

authors： Shelly DC,Warner IM,Quarles JM

更新日期：1980-09-01 00:00:00

abstract：:We review recently developed reference methods based on mass fragmentography (specific ion monitoring) with use of isotope-labeled internal standard, for determination of cholesterol, triglycerides, urea, glucose, cortisol, progesterone, and testosterone. With an optimal ratio between standard and material to be deter...

journal_title：Clinical chemistry

authors： Björkhem I,Blomstrand R,Lantto O,Svensson L,Ohman G

更新日期：1976-11-01 00:00:00

abstract：:Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detec...

journal_title：Clinical chemistry

authors： Flick JA,Schnaar RL,Perman JA

更新日期：1987-07-01 00:00:00

abstract：BACKGROUND:The time required for bloodstream pathogen detection, identification (ID), and antimicrobial susceptibility testing (AST) does not satisfy the acute needs of disease management. Conventional methods take up to 3 days for ID and AST. Molecular diagnostics have reduced times for ID, but their promise to suppla...

journal_title：Clinical chemistry

authors： Andini N,Hu A,Zhou L,Cogill S,Wang TH,Wittwer CT,Yang S

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

journal_title：Clinical chemistry

authors： Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

更新日期：2004-02-01 00:00:00

abstract：BACKGROUND:Cardiac troponins are associated with cardiovascular risk in the general population, but whether temporal changes in cardiac troponin I provide independent prognostic information remains uncertain. Using a large community-based cohort with follow-up close to the present day, we aimed to investigate the assoc...

journal_title：Clinical chemistry

authors： Lyngbakken MN,Røsjø H,Holmen OL,Dalen H,Hveem K,Omland T

更新日期：2019-07-01 00:00:00

abstract：BACKGROUND:Low concentrations of cardiac troponin (cTn) have been recommended for rapid rule-out of acute myocardial infarction (AMI). We examined the Beckman Coulter Access high-sensitivity cardiac troponin I (hs-cTnI) assay to identify a single test threshold that can safely rule out AMI. METHODS:This analysis used ...

journal_title：Clinical chemistry

authors： Greenslade J,Cho E,Van Hise C,Hawkins T,Parsonage W,Ungerer J,Tate J,Pretorius C,Than M,Cullen L

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:The genotype of hepatitis C virus (HCV) is a predictor of antiviral therapeutic response. We describe an approach for HCV genotype determination by real-time PCR and melting curve analysis. METHODS:After automated nucleic acid extraction, we used reverse transcription-PCR in a block cycler to amplify nucleo...

journal_title：Clinical chemistry

authors： Bullock GC,Bruns DE,Haverstick DM

更新日期：2002-12-01 00:00:00

abstract：:A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after ...

journal_title：Clinical chemistry

authors： Egberts J,Soederhuizen W,Gebhardt DO

更新日期：1983-02-01 00:00:00

abstract：:The clinical utilities of the emerging biochemical markers for mammary adenocarcinoma CA 15-3, CA 549, CA M26, CA M29, and MCA (mucin-like carcinoma-associated antigen) were assessed by a formal rating according to six desirable marker characteristics. All five indicators similarly have good specificities but limited ...

journal_title：Clinical chemistry

authors： Werner M,Faser C,Silverberg M

更新日期：1993-11-01 00:00:00

abstract：:We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly sim...

journal_title：Clinical chemistry

authors： Magnotti RA Jr,Stephens GW,Rogers RK,Pesce AJ

更新日期：1989-07-01 00:00:00

abstract：BACKGROUND:An accelerated stability model based on the Arrhenius Equation can be used to estimate stability of diagnostic reagents. Here we review 3 examples in which the model does not accurately predict the stability of diagnostic reagents. METHODS:We prepared several pilot lots of quality controls materials contain...

journal_title：Clinical chemistry

authors： Ebrahim A,DeVore K,Fischer T

更新日期：2020-12-04 00:00:00

abstract：:Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to form active beta-galactosidase. Using recombinant D...

journal_title：Clinical chemistry

authors： Henderson DR,Friedman SB,Harris JD,Manning WB,Zoccoli MA

更新日期：1986-09-01 00:00:00

abstract：BACKGROUND:An assay using a mouse antisialyl Lewis X (sLeX) antibody (CSLEX-1) is used clinically for screening and monitoring patients with breast cancer in Japan. However, the IgM isoform of CSLEX-1 is not preferred for the assay because the bulkiness of IgM generally causes poor accessibility to the antigen. To solv...

journal_title：Clinical chemistry

authors： Yamashita J,Kobayashi I,Tatematsu K,Sezutsu H,Noda K,Ishihara H

更新日期：2016-11-01 00:00:00

abstract：:Four routine assays commonly used for monitoring plasma methotrexate (MTX) during high-dose therapy were validated by HPLC as the comparison method. MTX and its main metabolite, 7-hydroxymethotrexate (7-OHMTX), were analyzed by HPLC with postcolumn derivatization and fluorometric detection. About 200 clinical plasma s...

journal_title：Clinical chemistry

authors： Albertioni F,Rask C,Eksborg S,Poulsen JH,Pettersson B,Beck O,Schroeder H,Peterson C

更新日期：1996-01-01 00:00:00

abstract：:On-line, simultaneous measurement of blood K+, Ca2+, Na+, and pH has been achieved by using a quadruple-function ChemFET (chemical-sensitive field-effect transistor) integrated-circuit sensor. Blood is withdrawn from the subject through an actively heparinized, dual-lumen cannula and passed into a flow-cell containing...

journal_title：Clinical chemistry

authors： Sibbald A,Covington AK,Carter RF

更新日期：1984-01-01 00:00:00

abstract：:Energy-dispersive x-ray spectroscopy and inductively coupled plasma emission spectrometry were evaluated as methods for routine multielement analysis of biological material. Standard samples included Standard Reference Materials (National Bureau of Standards), compounded mixtures, and supplements that provided a wide ...

journal_title：Clinical chemistry

authors： Irons RD,Schenk EA,Giauque RD

更新日期：1976-12-01 00:00:00

abstract：BACKGROUND:C-reactive protein (CRP) increases in response to inflammation and is purported to be a risk factor for atherogenesis. We recently demonstrated that a scavenger receptor, lectin-like oxidized LDL receptor (LOX-1), is a receptor for CRP. In light of the overlapping ligand spectrum of scavenger receptors such ...

journal_title：Clinical chemistry

authors： Fujita Y,Kakino A,Harada-Shiba M,Sato Y,Otsui K,Yoshimoto R,Sawamura T

更新日期：2010-03-01 00:00:00

abstract：BACKGROUND:One of the aims of cardiovascular genetics is to test the efficacy of the use of genetic information to predict cardiovascular risk. We therefore investigated whether inclusion of a set of common variants in candidate genes along with conventional risk factor (CRF) assessment enhanced coronary heart disease ...

journal_title：Clinical chemistry

authors： Humphries SE,Cooper JA,Talmud PJ,Miller GJ

更新日期：2007-01-01 00:00:00

abstract：:We evaluated the Kallestad "Quanticoat" cortisol RIA for direct (no extraction) measurement of urinary free cortisol, which requires no solvent extraction. An analytical-recovery study showed a linear regression of y (measured) = 0.65x (added) + 37.5 micrograms/L (Sy.x = 21.4 micrograms/L, r = 0.978, n = 48). Recovery...

journal_title：Clinical chemistry

authors： Huang CM,Zweig M

更新日期：1989-01-01 00:00:00

abstract：:We describe a direct radioimmunoassay for serotonin (5-hydroxytryptamine) in cerebrospinal fluid, platelet-poor plasma, and serum. We raised antisera in rabbits against serotonin diazotized to a conjugate of bovine albumin and D,L-p-aminophenylalanine. Polyethylene glycol, alone or in combination with anti-rabbit immu...

journal_title：Clinical chemistry

authors： Engbaek F,Voldby B

更新日期：1982-04-01 00:00:00

abstract：:We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wid...

journal_title：Clinical chemistry

authors： Wolf BA,Daft MC,Koenig JW,Flye MW,Turk JW,Scott MG

更新日期：1989-01-01 00:00:00