Abstract:
:A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint. Additional insight concerning the nature of the fluorescent pigments of these species is gained by analysis with a high-performance liquid chromatograph/video fluorometer combination. While providing information about the analytical variables contributing to the analysis, this combination also added the variable of chromatographic retention time for unambiguous fingerprinting.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Shelly DC,Warner IM,Quarles JMsubject
Has Abstractpub_date
1980-09-01 00:00:00pages
1419-24issue
10eissn
0009-9147issn
1530-8561journal_volume
26pub_type
历史文章,杂志文章abstract::We describe an automated enzymatic procedure for quantifying creatine in erythrocytes. In this assay, after sample clean-up, creatine kinase (CK; EC 2.7.3.2) and pyruvate kinase (EC 2.1.7.40) are used as auxiliary enzymes; lactate dehydrogenase (EC 1.1.1.27) is used in the indicator reaction. CK is also used as the st...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilita...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::Alanine aminotransferase activity in serum increases significantly when serum is incubated with pyridoxal phosphate. The increase depends on the L-alanine concentration in the final assay mixture, being greatest at 800 mmol/liter. Preincubation of 22 normal sera, in a 10:1 ratio with an 8.09 mmol/liter pyridoxal phosp...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-02-01 00:00:00
abstract::To secure a successful product in today's era of cost containment, manufacturers and developers of new in vitro diagnostic technologies must consider payment issues--coverage and reimbursement by healthcare insurers--as critical market forces that must be addressed in the business plan. Similar to the regulatory hurdl...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:The coexistence of hypertension and dyslipidemia synergistically increases the risk of cardiovascular events. We investigated the effect of the lipid-lowering agent micronized fenofibrate on inhibition of carotid atherosclerosis in patients with essential hypertension and mild hyperlipidemia. METHODS:We mea...
journal_title:Clinical chemistry
pub_type: 杂志文章,随机对照试验
doi:10.1373/clinchem.2006.074724
更新日期:2006-11-01 00:00:00
abstract::In photometry, where both the product formed and one or several reactants absorb light at the same wavelength, the absorbance of the "blank" of the sample at the end of the reaction may be less than that measured at the beginning of the reaction, because of consumption of reactant(s). The blank correction factor for t...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-03-01 00:00:00
abstract::We describe a simple method in which a water-soluble mixture of triocatanoin and a surfactant, "Triton X-114," are used in preparing solutions of triglycerides (triacylglycerols) in either human serum, solutions of albumin, or water. Analytical recovery added triglyceride was quantitative by two methods. The addition ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract:BACKGROUND:Technological advances have resulted in a renaissance of proteomic studies directed at finding markers of disease progression, diagnosis, or responsiveness to therapy. Renal diseases are ideally suited for such research, given that urine is an easily accessible biofluid and its protein content is derived mai...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2011.165969
更新日期:2012-02-01 00:00:00
abstract::The Dutch Foundation for Quality Assessment in Clinical Chemistry (SKZL) is the professional organization that conducts external quality-assessment schemes in The Netherlands. However, such schemes in fact assess the performance of the internal quality-control systems of the participating laboratories. In this paper w...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:The use of nonstandardized N-terminal pro-B-type natriuretic peptide (NT-proBNP) assays can contribute to the misdiagnosis of heart failure (HF). Moreover, there is yet to be established a common consensus regarding the circulating forms of NT-proBNP being used in current assays. We aimed to characterize and...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2012.200204
更新日期:2013-10-01 00:00:00
abstract::We have developed an interactive statistical quality-control system for the small- to medium-sized radioimmunoassay laboratory, which can be used in a programmable desk-top calculator instead of the medium- or large-scale computer systems usually required. The design of this quality-control system is modeled after the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract:BACKGROUND:The sex differential in coronary heart disease (CHD) risk, which is not explained by male/female differences in lipid and lipoprotein concentrations, narrows with age. We examined whether this differential CHD risk might, in part, be attributable to the sizes of lipoprotein particles or concentrations of lip...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.032763
更新日期:2004-07-01 00:00:00
abstract::Consecutive values for serum osmolality measured in a patient exceeded (548 mOsm) the previously reported pathological range and, indeed, exceeded that generally considered compatible with life. The patient was receiving large amounts of intravenous dimethyl sulfoxide as part of an experimental protocol to control int...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::Hemoglobinometry according to the International Committee of Standardization in Hematology (ICSH) suffers from imprecision related to high sample dilution and from potential errors owing to sample turbidity. We have evaluated a new instrument, "HemoCue," that measures hemoglobin at two wavelengths as azide methemoglob...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract::Applying the principles of chemometrics can lead to development of a powerful, simple, comprehensive system for characterizing measurement reliability of analytical processes. The system described here consists of a format for specifications, definitions, and decision rules for evaluating and comparing analytical proc...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
abstract::We examined the changes in sensitivity and specificity that would occur with alterations in the sample-collection schedule and (or) cutoff criterion for the increase in hydrogen concentration in breath after administration of doses of lactose in the dietary range. In a breath-analysis test to classify individuals as l...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
abstract::We evaluated a dual-precipitation method for determining cholesterol in high-density lipoprotein (HDL) and its subfractions HDL2 and HDL3. After total HDL was isolated by precipitation of very-low-density (VLDL) and low-density (LDL) lipoproteins with polyethylene glycol (Mr 8000), HDL2 was isolated from total HDL by ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::An oligonucleotide "melting" procedure was developed whereby we can monitor for a sequence heterogeneity in the gene for apolipoprotein E production. Two oligonucleotides were synthesized, one recognizing the common epsilon 3 allele but not the common epsilon 2 allele, the other recognizing the epsilon 2 allele but no...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract::This two-site immunoradiometric assay for human parathyrin-related protein 1-86 (PTHRP1-86) in plasma uses a mouse monoclonal antibody to PTHRP1-34 coupled to cellulose particles for immunoextraction of N-terminal immunoreactivity, and a rabbit antiserum to PTHRP37-67 that is indirectly labeled with 125I-labeled PTHRP...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
abstract::We determined estrogen receptor protein and carcinoembryonic antigen in cytosols prepared from 189 human breast carcinoma tissues, 85 benign or normal breast biopsies, and 101 tissue specimens metastatic from breast carcinoma. Carcinoembryonic antigen was observed in 70% of the primary carcinomatous tissues, 15% of th...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::Liquid scintillation counting of 3H-labeled whole-blood samples is severely impaired owing to quenching by blood pigments. In this study, dry oxidation and chemical solubilization followed by decolorization were the two general methods used to eliminate color quenching. Three blood volumes were examined: 0.25, 0.50, a...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-04-01 00:00:00
abstract::The basic theory of thyroxin-analog-based radioimmunoassays for free thyroxin has been extended to evaluate definitively the effects arising from residual binding of the tracer analog to serum proteins. Using experimentally determined binding constants and computer simulation techniques, we studied the effects of thyr...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract:BACKGROUND:We sought to develop a tandem mass spectrometry assay in which the enzymatic activities of 3 lysosomal enzymes (α-glucosidase, α-galactosidase A, and α-l-iduronidase) could be quantified in dried blood spots by using a single assay buffer. METHODS:A 3-mm dried blood spot punch was incubated in a single assa...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2010.152009
更新日期:2010-12-01 00:00:00
abstract::We have measured by reversed-phase HPLC concentrations of amino acids in plasma in groups of 80 normal appropriate-weight term babies fed from birth either a casein formula (WhiteCap SMA, n = 26), a whey formula (Gold Cap SMA, n = 26), or breast milk (n = 28). They were studied from day 11 to week 15 postpartum. The t...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:High-sensitivity C-reactive protein (hsCRP) testing is relatively inexpensive and has been shown to predict the risk of cardiovascular disease (CVD) and diabetes in multiple patient groups, including those treated with statin therapy. JUPITER (Justification for the Use of Statins in Primary Prevention: An In...
journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2008.109728
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND:Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. METHODS:We used layer-by-layer technology ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2005.065789
更新日期:2006-08-01 00:00:00
abstract::A gas-chromatographic method for urinary organic acid analysis is described, designed to be used routinely for the diagnosis of organic aciduria. It involves extraction of urine with ethyl acetate, dehydration of extract residues, and trimethylsilylation. Organic acids are identified by using an extensive list of rete...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::In this report on trace-element concentrations (As, Ca, Cd, Cl, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Rb, Sb, Se, Zn) in human heart, liver, kidney, aorta, and rib obtained from 200 autopsied patients, we give special attention to sampling procedure, analysis technique, and various sources of error (autolysis...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::The formal definition of sensitivity associates the term with the change in the response of a system for a small change of the stimulus causing the response, i.e., the ratio of the response of a system to the stimulus causing it. One interpretation of sensitivity associates the rate of change of the response for a sma...
journal_title:Clinical chemistry
pub_type: 评论,杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract:BACKGROUND:The gene that causes most cases of hereditary hemochromatosis is designated HFE. Individuals with mutations in the HFE gene may have increased serum iron, transferrin saturation, and ferritin concentrations relative to individuals with the wild-type genotype. METHODS:We generated reference centiles for perc...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2001-10-01 00:00:00