Improved continuous-flow method for detemination of total serum hexosamines.

Abstract:

:We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilitate the detemination as compared to the 4-h hydrolysis used in the conventional manual method. Results correlated well (r = 0.906) with those obtained by the corresponding manual method.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Sobocinski PZ,Canterbury WJ,Jurgens KH

subject

Has Abstract

pub_date

1976-08-01 00:00:00

pages

1394-6

issue

8

eissn

0009-9147

issn

1530-8561

journal_volume

22

pub_type

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