Abstract：
BACKGROUND:The sex differential in coronary heart disease (CHD) risk, which is not explained by male/female differences in lipid and lipoprotein concentrations, narrows with age. We examined whether this differential CHD risk might, in part, be attributable to the sizes of lipoprotein particles or concentrations of lipoprotein subclasses. METHODS:We analyzed frozen plasma samples from 1574 men and 1692 women from exam cycle 4 (1988-1990) of the Framingham Offspring Study. Nuclear magnetic resonance (NMR) spectroscopy was used to determine the subclass concentrations and mean sizes of VLDL, LDL, and HDL particles. Concentrations of lipids and apolipoproteins were measured by standard chemical methods. RESULTS:In addition to the expected sex differences in concentrations of triglycerides, LDL-cholesterol, and HDL-cholesterol, women also had a lower-risk subclass profile consisting of larger LDL (0.4 nm) and HDL (0.5 nm) particles. The sex difference was most pronounced for HDL, with women having a twofold higher (8 vs 4 micromol/L) concentration of large HDL particles than men. Furthermore, similar to the narrowing of the sex difference in CHD risk with age, the observed male/female difference in HDL particle size also decreased with age. Although lipoprotein particle sizes were highly correlated with lipid and lipoprotein concentrations, the sex differences in the mean sizes of lipoprotein particles persisted (P <0.001) even after adjustment for lipid and lipoprotein concentrations. CONCLUSIONS:Women have a less atherogenic subclass profile than men, even after accounting for differences in lipid concentrations.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Freedman DS,Otvos JD,Jeyarajah EJ,Shalaurova I,Cupples LA,Parise H,D'Agostino RB,Wilson PW,Schaefer EJdoi
10.1373/clinchem.2004.032763subject
Has Abstractpub_date
2004-07-01 00:00:00pages
1189-200issue
7eissn
0009-9147issn
1530-8561pii
clinchem.2004.032763journal_volume
50pub_type
杂志文章abstract：BACKGROUND:To determine the contribution of laboratory costs to the overall costs of managing hospital patients with different diseases, we studied the costs of laboratory testing overall and in relation to the other costs incurred during hospitalization. METHODS:We used a database developed by the University HealthSy...
journal_title：Clinical chemistry
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abstract：:Polyacrylamide has been the matrix of choice for isoelectric focusing owing to the virtual absence of electroendosmosis in this medium. Certain inherent limitations associated with polyacrylamide have prompted some investigators to use low-electroendosmosis agarose for isoelectric focusing, but with limited success th...
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abstract：:We have developed an HPLC method for measuring carbamylated hemoglobin (CarHb), based on the quantification of valine hydantoin formed from the released NH2-terminal carbamyl valine residue after acid hydrolysis of hemoglobin. In uremia, CarHb is produced by nonenzymatic post-translational modification of the terminal...
journal_title：Clinical chemistry
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abstract：:In this rapid, simple enzyme immunoassay for carcinoembryonic antigen (CEA) in serum the "sandwich" system is used. The CEA is first extracted from the serum into acetate buffer by heating at 70 degrees C, then incubated in a polyvinyl microtitration plate previously coated with anti-CEA. The bound CEA is then reacted...
journal_title：Clinical chemistry
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abstract：:A new radiometric assay specific for creatine kinase isoenzyme MB was evaluated with respect to its precision and agreement with a conventional electrophoretic CK-MB assay for the diagnosis of myocardial infarction. The reference interval we find for serum CK-MB in healthy subjects is 0--30 micrograms/L. The coefficie...
journal_title：Clinical chemistry
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abstract：:In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No s...
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
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更新日期：1984-07-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1993-06-01 00:00:00
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journal_title：Clinical chemistry
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doi：
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journal_title：Clinical chemistry
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doi：
更新日期：1993-09-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1979-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1993-06-01 00:00:00
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doi：
更新日期：1986-06-01 00:00:00
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更新日期：2006-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1994-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
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doi：
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doi：
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更新日期：1987-09-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1988-12-01 00:00:00
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