Abstract：
BACKGROUND:Remnant lipoprotein-cholesterol (RLP-C) concentrations in sera of healthy individuals are very low (0.080-0.437 mmol/L), making conventional cholesterol methods poorly suited to this purpose. We have developed a highly sensitive cholesterol assay (CD method) and applied it to the RLP-C assay. METHODS:The CD shuttled cholesterol reversibly between reduced and oxidized forms in the presence of thio-NAD and NADH. The production rate of thio-NADH correlated with the cholesterol concentration and was measured by the absorbance at 404/500 nm. This CD method was combined with an immunoaffinity separation procedure with specific monoclonal antibodies to apolipoprotein (apo) A1 and apo B-100 and used for RLP-C assay. Results were compared with a RLP-C method that uses cholesterol oxidase, peroxidase, and chromogenic substrate. RESULTS:The CD method could detect 0.10 x 10(-3) mmol/L cholesterol and was at least 5 times more sensitive than the conventional enzymatic method. Within- and between-day imprecision (as CVs) of the RLP-C assay with the CD method was <4%. Regression analysis of RLP-C assays with the new (y) and conventional (x) cholesterol methods yielded: y = 1.02x - 0.008 mmol/L (S(y/x) = 0.0065 mmol/L; r = 0.997; n = 297). CONCLUSIONS:Serum RLP-C can be measured by the CD method. The CD method may be useful for other assays that require sensitive cholesterol measurements in biological materials.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Kishi K,Ochiai K,Ohta Y,Uemura Y,Kanatani K,Nakajima K,Nakamura Msubject
Has Abstractpub_date
2002-05-01 00:00:00pages
737-41issue
5eissn
0009-9147issn
1530-8561journal_volume
48pub_type
杂志文章abstract：:Use of immunoassays that do not detect endogenous digoxin-like immunoreactive factors (DLIF) in serum significantly improves the between-assay correlation of digoxin results for patients. We investigated five different immunoassay methods (Abbott, Clinical Assays, Corning, Du Pont, and Syva), measuring digoxin by all ...
journal_title：Clinical chemistry
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doi：
更新日期：1987-06-01 00:00:00
abstract：:Applying the principles of chemometrics can lead to development of a powerful, simple, comprehensive system for characterizing measurement reliability of analytical processes. The system described here consists of a format for specifications, definitions, and decision rules for evaluating and comparing analytical proc...
journal_title：Clinical chemistry
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更新日期：1988-12-01 00:00:00
abstract：:Single-spin density-gradient ultracentrifugation (DUC) has proven to be a reproducible method for detection of low-density-lipoprotein (LDL) heterogeneity. Recently another method has been described for this: gradient gel electrophoresis (GGE) of serum, a method that might be more suitable for screening. To gain insig...
journal_title：Clinical chemistry
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更新日期：1991-06-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1993-08-01 00:00:00
abstract：:We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for ...
journal_title：Clinical chemistry
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doi：
更新日期：1989-05-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1988-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1985-03-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1995-03-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1976-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-08-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1991-02-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.061267
更新日期：2006-04-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O-carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and a...
journal_title：Clinical chemistry
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doi：
更新日期：1979-05-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2005.065417
更新日期：2006-08-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1982-04-01 00:00:00
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journal_title：Clinical chemistry
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doi：10.1373/clinchem.2011.175570
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journal_title：Clinical chemistry
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1992-09-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1985-06-01 00:00:00
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journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1975-10-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1988-01-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1979-11-01 00:00:00
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journal_title：Clinical chemistry
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doi：
更新日期：1978-09-01 00:00:00
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journal_title：Clinical chemistry
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更新日期：2019-12-01 00:00:00
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journal_title：Clinical chemistry
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