Abstract:
BACKGROUND:Although numerous lateral flow immunoassays (LFIAs) have been developed and widely used, inadequate analytical sensitivity and the lack of multiple protein detection applications have limited their clinical utility. We developed a new LFIA device for the simultaneous detection of high-sensitivity cardiac troponin I (hs-cTnI) and myoglobin (Myo). METHODS:We used a gold nanoparticle (AuNP) doubly labeled complex, in which biotinylated single-stranded DNA was used as a linkage to integrate 2 AuNPs and streptavidin-labeled AuNP, as an amplifier to magnify extremely low signals. RESULTS:The detection limit of 1 ng/L achieved for hs-cTnI was 1000 times lower than that obtained in a conventional LFIA. The detection limit for simultaneously measured Myo was 1 μg/L. The linear measurement ranges for hs-cTnI and Myo were 1-10 000 ng/L and 1-10 000 μg/L, respectively. We observed concordant results between the LFIA and clinical assays in sera from 12 patients with acute myocardial infarction (hs-cTnI r = 0.96; Myo r = 0.98). Assay imprecision was <11% for both hs-TnI and myo. CONCLUSIONS:The described proof-of-principle LFIA method could be used as a point-of-care device in multiple protein quantification and semiquantitative analysis.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Zhu J,Zou N,Zhu D,Wang J,Jin Q,Zhao J,Mao Hdoi
10.1373/clinchem.2011.171694subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1732-8issue
12eissn
0009-9147issn
1530-8561pii
clinchem.2011.171694journal_volume
57pub_type
杂志文章abstract:BACKGROUND:A simple point-of-care method for measuring leukocyte counts in a doctor's office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils ...
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journal_title:Clinical chemistry
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