Abstract:
BACKGROUND:Thrombopoietin (TPO), the major hormone controlling platelet production, has been measured in thrombocytopenias with discordant results. The aim of our work was to assess the value of the TPO assay for differential diagnosis of thrombocytopenias in a large cohort of patients classified according to the results of their platelet isotopic study. METHODS:We measured TPO (R&D Systems) in serum of 160 thrombocytopenic patients referred to our department for platelet life span isotopic studies. We classified patients as follows: (a) idiopathic or autoimmune thrombocytopenia group (ITP; patients with increased platelet destruction and shortened platelet life span; n = 67); (b) pure genetic thrombocytopenia group (patients with decreased platelet production, normal platelet life span, and without bone marrow aplasia; n = 55); (c) bone marrow aplasia group (BM; patients with decreased platelet production, normal platelet life span, and bone marrow aplasia; n = 13). RESULTS:In patients with pure genetic thrombocytopenia, TPO (median, 55 ng/L) was not different from TPO in patients with ITP (median, 58 ng/L) or controls (n = 54; median, 51 ng/L). Only in patients with bone marrow aplasia was TPO significantly higher (median, 155 ng/L) and negatively correlated to the platelet count (r(2) = 0.5014). CONCLUSIONS:Although the median serum TPO is increased in thrombocytopenia with decreased platelet production from bone marrow aplasia, it does not differentiate patients with pure genetic thrombocytopenia from those with ITP.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Gouin-Thibault I,Cassinat B,Chomienne C,Rain JD,Najean Y,Schlageter MHsubject
Has Abstractpub_date
2001-09-01 00:00:00pages
1660-5issue
9eissn
0009-9147issn
1530-8561journal_volume
47pub_type
杂志文章abstract::For normal and above-normal concentrations of angiotensin-converting enzyme (ACE; EC 3.4.15.1) activity in plasma, results of a manual fluorometric method [with hippuryl-histidyl-leucine (HHL), 5 mmol/L, as substrate] correlated well with those of an automated spectrophotometric method [with 3-(2-furylacryloyl)-L-phen...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Based on the quantitative determination of creatinine, total protein, albumin, alpha 1-microglobulin, IgG, alpha 2-macroglobulin, and N-acetyl-beta, D-glucosaminidase in urine in combination with a test strip screening, the findings of hematuria, leukocyturia, and proteinuria can be assigned to prerenal, renal, or pos...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:10.1373/clinchem.2008.112151
更新日期:2009-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::A simple computer-based screening technique has been developed for classifying human expired air components into 16 chemical classes, based on empirical formulas. The sort procedure was developed to simplify the screening of the composition of expired air samples by sorting all components into chemical classes and cla...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章,评审
doi:10.1373/clinchem.2011.165704
更新日期:2012-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1993-09-01 00:00:00
abstract::We evaluated superoxide dismutase (EC 1.15.1.1) and glutathione peroxidase (EC 1.11.1.9) activities in the erythrocytes of lead-exposed and nonexposed workers from distinct industries localized in the State of São Paulo, Brazil. Concentrations of either lead or protoporphyrin IX, or both, in blood were used to indicat...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract:BACKGROUND:We investigated agreement between self-reported prenatal alcohol exposure (PAE) and objective meconium alcohol markers to determine the optimal meconium marker and threshold for identifying PAE. METHODS:Meconium fatty acid ethyl esters (FAEE), ethyl glucuronide (EtG), and ethyl sulfate (EtS) were quantified...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2014.233718
更新日期:2015-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.079012
更新日期:2007-04-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.1.51
更新日期:2003-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-02-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1979-04-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1987-04-01 00:00:00
abstract::Cardiac troponin I (cTnI) is a regulatory protein unique to myocardium. We used a cardiospecific 30-min ELISA to measure cTnI in EDTA-plasma samples serially drawn from 28 patients before and after coronary artery bypass grafting (CABG)--26 elective and 2 salvage cases. The cTnI increase in 22 of the elective CABG pat...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::Circulating prostate cells can be detected in cancer patients by using reverse transcriptase-PCR (RT-PCR) assay for prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) mRNA. A quality-control study involving a conventional RT-PCR assay was performed and, surprisingly, detected both transcripts...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2007.089102
更新日期:2007-10-01 00:00:00