Abstract:
BACKGROUND:Gas6 is a vitamin K-dependent antiapoptotic protein that has been implicated in cardiovascular pathophysiology. We report the development and validation of an ELISA for Gas6, and the variation of plasma Gas6 with hormonal status in a study designed to evaluate the effect of oral contraception on plasma markers. METHODS:After validation of the main stages of the ELISA assay, we measured plasma Gas6 concentrations in 94 male and 88 female healthy volunteers ages 18 to 38 years. Forty-five of the women then received an oral contraceptive, which contained ethinylestradiol and levonorgestrel, for 3 months before a new measurement was performed at the same time point in their menstrual cycles. RESULTS:Interassay imprecision was 5.8%-11.8%, and the detection limit was 5.9 microg/L. Mean Gas6 plasma concentrations were significantly lower in men (52.0 microg/L) than in women not receiving oral contraceptives (63.8 microg/L, P <0.001). In the women who received oral contraceptives, Gas6 concentrations decreased after 3 months of therapy from 63.6 microg/L to 51.9 microg/L (P <0.001). CONCLUSIONS:We have developed a simple and reproducible ELISA assay for measuring plasma Gas6 concentrations, which vary with sex and are decreased by oral contraceptive use. These results suggest regulation of plasma Gas6 concentrations by sex hormones. Future clinical studies may require participants to be stratified by sex.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Clauser S,Peyrard S,Gaussem P,Crespin M,Emmerich J,Aiach M,Borgel Ddoi
10.1373/clinchem.2007.089102subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1808-13issue
10eissn
0009-9147issn
1530-8561pii
clinchem.2007.089102journal_volume
53pub_type
杂志文章abstract::We report microchromatographic measurement of fetal hemoglobin (HbF) proportions in a 36-year-old African-American multigravida woman. At 34 weeks she delivered a 630-g male infant who subsequently did well. Hemoglobin electrophoresis of the hemolysate revealed nearly 100% HbF without HbA, an extremely unusual natural...
journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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更新日期:1995-02-01 00:00:00
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1976-10-01 00:00:00
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doi:
更新日期:2002-11-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1992-12-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1983-10-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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更新日期:1980-09-01 00:00:00
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更新日期:1980-11-01 00:00:00
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