Abstract:
:"TDx Fetal Lung Maturity," an automated assay that measures the relative concentrations of surfactant and albumin in amniotic fluid, was compared with the lecithin/sphingomyelin (L/S) ratio and phosphatidylglycerol determination at five clinical sites. A total of 695 amniotic fluid samples were analyzed, of which 312 were followed by delivery of the infant within three days of sample collection. Of these 312, 24 developed respiratory distress syndrome and seven developed transient tachypnea. With the cutoff for maturity set at a surfactant/albumin value of 50 mg/g, the assay showed a sensitivity of 0.96 and a specificity of 0.88 for all samples, compared with a sensitivity of 0.96 and a specificity of 0.83 for the L/S ratio. The combination of rapid assay (30 min), accurate results, and uniformity among testing centers makes the TDx assay a very promising method.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Russell JC,Cooper CM,Ketchum CH,Torday JS,Richardson DK,Holt JA,Kaplan LA,Swanson JR,Ivie WMsubject
Has Abstractpub_date
1989-06-01 00:00:00pages
1005-10issue
6eissn
0009-9147issn
1530-8561journal_volume
35pub_type
杂志文章abstract::Radiometric, colorimetric, and two immunochemical methods for measuring total iron-binding capacity are compared. We evaluated the procedures on the basis of precision, applicability to a pediatric population, and accuracy as assessed by analytical recovery of purified transferrin. The immunoephelometric assay for tra...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::We describe a completely automated enzymic system for measuring total cholesterol in serum. All reagents are contained in an analytical test pack and the test is performed on Du Pont's Automatic Clinical Analyzer (aca), which mixes the sample (20 microliter) and reagents and performs the necessary absorbance measureme...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::In "flow-injection analysis," the sample is inserted into a stream of reagent by use of a sample-injection valve. Mixing occurs downstream from the valve in a coil of tubing. With chemiluminescence detection this coil is positioned in front of a photomultiplier. We have evaluated the system for detection of hydrogen p...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract:BACKGROUND:Screening for heterozygous sequence changes in PCR products, also known as "mutation scanning", is an important tool for genetic research and clinical applications. Conventional methods require a separation step. METHODS:We evaluated the sensitivity and specificity of homogeneous scanning, using a saturatin...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2003.029751
更新日期:2004-10-01 00:00:00
abstract::Noninvasive monitoring of blood/tissue glucose concentrations has been successfully accomplished in individual diabetic subjects by using near-infrared (NIR) spectroscopy coupled with chemometric methods. Three different spectrometer configurations were tested: a) a Fourier-transform infrared spectrometer with an indi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-09-01 00:00:00
abstract::We report microchromatographic measurement of fetal hemoglobin (HbF) proportions in a 36-year-old African-American multigravida woman. At 34 weeks she delivered a 630-g male infant who subsequently did well. Hemoglobin electrophoresis of the hemolysate revealed nearly 100% HbF without HbA, an extremely unusual natural...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::We followed the "abbreviated precision protocol" of the National Committee for Clinical Laboratory Standards for the evaluation of precision, accuracy, and carryover in analyses for glucose with the "Ektachem." We analyzed 760 clinical samples by this technique, by the FDA Proposed Class Standard glucose reference met...
journal_title:Clinical chemistry
pub_type: 临床试验,杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::A two-site assay was developed by use of the "dissociation and enhancement lanthanide fluoroimmunoassay" (DELFIA) technique for determination of salmon calcitonin (SCT) in serum after administration to osteoporotic patients. Polyclonal antibodies were produced in rabbits immunized with SCT coupled to ovalbumin. After ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::We devised a method for assaying serum lactate dehydrogenase isoenzyme 1 (LD-1) activity specifically by preincubation with alpha-chymotrypsin and guanidine. Cleavage of phenylalanine bonds in the loop of A and B subunits of LD-3, LD-4, and LD-5 isoenzymes (residues 117-119) by incubation with alpha-chymotrypsin for a...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::We describe a gas--liquid-chromatographic method for determining the concentration of amantadine hydrochloride in urine with beta-phenylethylamine as internal standard. The urine sample is made alkaline and extracted with 0.5 mL of chloroform. After centrifugation the aqueous layer is aspirated, and an aliquot of the ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1980-02-01 00:00:00
abstract:BACKGROUND:Age-adjusted morbidity and mortality rates from coronary heart disease (CHD) are higher in men than in women. Androgens are suspected to be responsible for the male disadvantage. The genomic effect of androgens is mediated by the androgen receptor (AR), which has a polymorphic CAG repeat in exon 1. The numbe...
journal_title:Clinical chemistry
pub_type: 杂志文章,多中心研究
doi:10.1373/clinchem.2005.049262
更新日期:2005-07-01 00:00:00
abstract::We have developed an HPLC method for measuring carbamylated hemoglobin (CarHb), based on the quantification of valine hydantoin formed from the released NH2-terminal carbamyl valine residue after acid hydrolysis of hemoglobin. In uremia, CarHb is produced by nonenzymatic post-translational modification of the terminal...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract:BACKGROUND:The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO(2) particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). ME...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2006.071555
更新日期:2006-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::To investigate the use of streptavidin-hapten derivatives as potential protein-tracer conjugates for competitive-type immunoassays, we labeled streptavidin with cortisol and compared biotin-binding activity of the conjugates with that of unlabeled streptavidin. In this model system, streptavidin labeled with one to ap...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::I describe a micro-scale method for determining lead in whole blood by utilizing a graphite furnace. Sample pretreatment consists of fivefold dilution with a dilute surfactant. The method is directly calibrated with lead standards prepared in dilute HNO3. To eliminate a small, nonspecific absorption signal from the...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1975-04-01 00:00:00
abstract::In this automated apoenzyme reactivation immunoassay system (Ames Optimate) for thyroxin-binding globulin (TBG), the sample and N6-aminohexylflavin adenine dinucleotide-labeled TBG react sequentially with antiserum. Then apoglucose oxidase is added to combine with the free fraction and generate glucose oxidase activit...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2005.061838
更新日期:2006-04-01 00:00:00
abstract::We have developed two methods for determining serum concentrations of felbamate, a new anticonvulsant drug. The first method is based on protein precipitation with acetonitrile, followed by HPLC. The between-run CV for this method is 5.7% (mean 55 mg/L), and the linearity extends from 5 to 175 mg/L. Results by this me...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::We have reported (Clin Chem 29: 42-44, 1983) that prestaining with Remazol Brilliant Blue permits direct visualization of serum components on disc electrophoresis, and apparently purifies the proteins well. Here we have cut out the bands corresponding to the prestained albumin and transferrin after disc electrophoresi...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1984-07-01 00:00:00
abstract::Numerous papers have been written to show which combinations of Shewhart-type quality-control charts are optimal for detecting systematic shifts in the mean response of a process, increases in the random error of a process, and linear drift effects in the mean response across the assay batch. One paper by Westgard et ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract:BACKGROUND:Some of the genetic variants of hemoglobin (Hb) and their chemically modified species are known to affect the measurement of Hb A(1c). The purpose of this study was to characterize Hb species in the blood sample of a 74-year-old German male with an exceptionally low Hb A(1c) value. METHODS:Hemolysates from ...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/49.1.137
更新日期:2003-01-01 00:00:00
abstract::I determined the in vitro biological half-lives or decay constants for creatine kinase isoenzymes at various temperatures. Values at 37 degrees C are consistent with values reported by others in vivo, which suggests that in vivo irreversible inactivation is primarily thermal. Reversible inactivation appears to be an o...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00
abstract::Two-dimensional electroimmunodiffusion is rarely used in clinical chemistry, because its potentialities are not yet widely appreciated. Studying mouse anaphylaxis, we observed with this technique certain striking short- and long-term pathologic changes in several important serum antigens, which aptly demonstrate its u...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1979-05-01 00:00:00
abstract::We describe a solid-phase radioimmunometric sandwich assay for a new tumor marker defined by a monoclonal antibody (19-9). This antibody reacts with a carbohydrate antigenic determinant (CA 19-9) found at low concentrations in sera from healthy individuals but frequently increased in sera from patients with adenocarci...
journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1983-03-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:10.1373/clinchem.2004.037630
更新日期:2005-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00