Abstract：
:This HPLC/immunoassay procedure measures digoxin in serum with no interference from digoxin metabolites or digoxin-like factors. We used solid-phase (C18 and Diol) extraction, a C18 column with a tetrahydrofuran/water mobile phase, and final quantification by fluorescence polarization immunoassay. Deslanoside and gitoxigenin were used as the internal standard and the retention-time marker, respectively. The average CV for 300-microL samples at digoxin concentrations between 0.9 and 3.9 nmol/L was 9.3%. Minimum column lifetime with daily use was three months. We also compared results, for 49 samples from patients taking digoxin, obtained with the Abbott "TDx FPIA digoxin I" and the present procedure. Discrepancies between the two methods were substantial for 20% of the samples.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Stone JA,Soldin SJsubject
Has Abstractpub_date
1988-12-01 00:00:00pages
2547-51issue
12eissn
0009-9147issn
1530-8561journal_volume
34pub_type
杂志文章abstract：:We compared three current methods (immunoinhibition, "Isomune-CK" immunoprecipitation, and the Tandem-E CKMB II immunoenzymometric assay) for determination of creatine kinase (CK; EC 2.7.3.2) isoenzyme MB in serum. Although results inter-correlated well, the immunoinhibition assay gave higher activity values. Atypical...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:The TDx Fetal Lung Maturity II (FLM II) assay uses amniotic fluid to assess lung maturity of the unborn infant. We investigated common preanalytical factors that influence FLM results, including centrifugation, sample storage, and contamination by whole blood. METHODS:We tested 18 specimens after centrifuga...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/49.6.935
更新日期：2003-06-01 00:00:00
abstract：:We describe the sensitive and precise determination 5-hydroxytryptophan in plasma from patients treated with this compound. The method is based on solvent extraction of plasma, high-performance liquid chromatography and continuous fluorometric detection after reaction with phthaldialdehyde in strong acid. The combined...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Red blood cell (RBC) replacement solutions are being developed as alternatives to allogeneic RBC use in blood transfusions in the treatment of massive trauma, to achieve hemodynamic stability during elective surgery, and to increase oxygen-carrying capacity in anemia. Hemoglobin-based oxygen carrier (HBOC)-201 (Biopur...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
abstract：BACKGROUND:The purpose of this study was to assess the feasibility of infrared (IR) spectroscopy for the simultaneous quantification of serum LDL-cholesterol (LDL-C) and HDL-cholesterol (HDL-C) concentrations. METHODS:Serum samples (n = 90) were obtained. Duplicate aliquots (5 microL) of the serum specimens were dried...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:We present evidence for the utility of an improved assay for the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes 1 and 2 in serum, involving inhibition of the H-subunit of LD by pyruvate at pH 7.1. Results correlate well with the LD-1/total LD ratio as evaluated by immunological assay and, as an index to in...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:Noninvasive monitoring of blood/tissue glucose concentrations has been successfully accomplished in individual diabetic subjects by using near-infrared (NIR) spectroscopy coupled with chemometric methods. Three different spectrometer configurations were tested: a) a Fourier-transform infrared spectrometer with an indi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：BACKGROUND:Reduced renal function is an established risk factor for cardiovascular events. We compared 3 measures of renal function--serum cystatin C, serum creatinine, and calculated creatinine clearance--as predictors of subsequent cardiovascular events in a community-based population of elderly individuals. METHODS...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.135962
更新日期：2010-05-01 00:00:00
abstract：:Consecutive values for serum osmolality measured in a patient exceeded (548 mOsm) the previously reported pathological range and, indeed, exceeded that generally considered compatible with life. The patient was receiving large amounts of intravenous dimethyl sulfoxide as part of an experimental protocol to control int...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：BACKGROUND:Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. ME...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2009.129833
更新日期：2009-11-01 00:00:00
abstract：BACKGROUND:alpha(1)-Antitrypsin is the major plasma serine protease inhibitor. Its deficiency is mainly associated with the alleles PI*S and PI*Z and can lead to obstructive lung disease in adults and to liver cirrhosis during childhood. METHODS:A multiplex PCR method has been established that uses two sets of primers...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：2000-02-01 00:00:00
abstract：:In "flow-injection analysis," the sample is inserted into a stream of reagent by use of a sample-injection valve. Mixing occurs downstream from the valve in a coil of tubing. With chemiluminescence detection this coil is positioned in front of a photomultiplier. We have evaluated the system for detection of hydrogen p...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1979-09-01 00:00:00
abstract：:Four routine assays commonly used for monitoring plasma methotrexate (MTX) during high-dose therapy were validated by HPLC as the comparison method. MTX and its main metabolite, 7-hydroxymethotrexate (7-OHMTX), were analyzed by HPLC with postcolumn derivatization and fluorometric detection. About 200 clinical plasma s...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanol-borate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detec...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1987-07-01 00:00:00
abstract：:A profile (chromatographic pattern) of the neutral and acidic metabolites present in cerebrospinal fluid from newborns and infants was obtained by gas-liquid chromatography. The metabolites are those extracted with ethyl acetate and diethyl ether. They are converted to trimethylsilyl ether derivatives, and chromatogra...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1976-05-01 00:00:00
abstract：:Two families with benign hyperphosphatasemia of intestinal origin were studied and compared with six other cases reported in the literature. No evidence of clinical abnormalities or explanations for the unusual enzyme concentrations were found. Agarose gel electrophoresis of alkaline phosphatase (ALP, EC 3.1.3.1) isoe...
journal_title：Clinical chemistry
pub_type： 杂志文章,评审
doi：
更新日期：1991-08-01 00:00:00
abstract：:We have developed a series of photochemical silver stains that are more than 100 times as sensitive as the Coomassie Blue stains. These stains facilitate the quantitative estimation of proteins in two-dimensional gel patterns. Recently, we discovered that by using light (photodevelopment) to catalyze the reduction of ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
abstract：:We review atomic flame spectrometry of metals based on the selective laser-enhancement of the rate of atomic ionization. A discussion of basic principles, prior literature, and instrumentation is followed by an overview of analytical figures of merit for the technique, and a look at its present status and areas for fu...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1983-09-01 00:00:00
abstract：BACKGROUND:Multiplexed amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, asymmetric PCR, or allele-specific PCR; however, correct differentiation of homozygous mutant and wild-type samples by melting temperature (T(m)) analysis requires high-resolution melting ana...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2007.097121
更新日期：2008-01-01 00:00:00
abstract：BACKGROUND:PCR-based detection of minimal residual disease (MRD) in neuroblastoma (NB) patients can be used for initial staging and monitoring therapy response in bone marrow (BM) and peripheral blood (PB). PHOX2B has been identified as a sensitive and specific MRD marker; however, its expression varies between tumors....
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2008.117945
更新日期：2009-07-01 00:00:00
abstract：:Problems encountered in the uroporphyrinogen I synthase assay were found to be due to the Triton reagent, slit-width and excitation wavelength settings of the spectrophotofluorometer, and leg-phase in the enzyme reaction. Of Triton X-100 from four suppliers, only three were found suitable. Differences in enzyme activi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:I evaluated use of the fluorescence polarization technique to measure neocarzinostatin, a proteinaceous antitumor antibiotic, and its antibody, in serum. The antigen (neocarzinostatin), labeled with fluorescein isothiocyanate, was allowed to interact with its antibody in a cuvet, in the instrument, yielding an increas...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-12-01 00:00:00
abstract：BACKGROUND:Recent studies have suggested that glial fibrillary acidic protein (GFAP) serum concentrations distinguish between intracerebral hemorrhage (ICH) and ischemic stroke (IS) shortly after symptom onset. In this prospective multicenter trial we validated GFAP in an independent patient cohort and assessed the qua...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2016.263335
更新日期：2017-01-01 00:00:00
abstract：:We evaluated a new EMIT II monoclonal amphetamine/methamphetamine assay for screening human urine by comparing it with the EMIT d.a.u. monoclonal amphetamine/methamphetamine assay and a fluorescence polarization assay. The EMIT II assay has a cutoff of 1 mg/L d-methamphetamine. The EMIT II and EMIT d.a.u. assays were ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Liquid scintillation counting of 3H-labeled whole-blood samples is severely impaired owing to quenching by blood pigments. In this study, dry oxidation and chemical solubilization followed by decolorization were the two general methods used to eliminate color quenching. Three blood volumes were examined: 0.25, 0.50, a...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1981-04-01 00:00:00
abstract：:Histograms of the results of quantitative clinical laboratory analyses are usually broadly skewed because some of the patients have normal values with respect to the analyte being measured while values for others are below or above normal. We describe a technique for calculating the maximum possible number of individu...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1978-04-01 00:00:00
abstract：BACKGROUND:Atherosclerosis is a complex, chronic disease that usually arises from the converging action of several pathogenic processes, including hypertension, hyperlipidemia, obesity, and the accumulation of oxidized LDL. Platelet-activating factor acetylhydrolase (PAF-AH) is a LDL- and HDL-bound enzyme that hydrolyz...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2004.036863
更新日期：2004-11-01 00:00:00
abstract：:We isolated myoglobin from sheep heart by homogenizing cardiac muscle in 70%-saturated ammonium sulfate, followed by chromatography on a column containing carboxymethyl(CM)-Sephadex gel. Two major isoforms of myoglobin, designated Mb 7.9 and Mb 8.1, were separated by chromatofocusing and were distinguished by their di...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:The immunoreactivity of anti-triiodothyronine (anti-T3) IgG, pretreated at acidic pH and adsorbed onto polystyrene beads, was significantly greater than that determined for native anti-T3 IgG immobilized at optimum pH 3.5. Acidic-pH-pretreated IgG, adsorbed at pH 7.0 onto ethanol-treated beads, had less immunoreactivi...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：BACKGROUND:ADB-PINACA and its 5-fluoropentyl analog 5F-ADB-PINACA are among the most potent synthetic cannabinoids tested to date, with several severe intoxication cases. ADB-PINACA and 5F-ADB-PINACA have a different legal status, depending on the country. Synthetic cannabinoid metabolites predominate in urine, making ...
journal_title：Clinical chemistry
pub_type： 杂志文章
doi：10.1373/clinchem.2016.267575
更新日期：2017-05-01 00:00:00
