Comparison of urinary albumin quantification by immunoturbidimetry, competitive immunoassay, and protein-cleavage liquid chromatography-tandem mass spectrometry.

Abstract:

BACKGROUND:Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. METHODS:We quantified urinary albumin in patient samples by using 3 commercially available reagent systems: DiaSorin SPQ and Beckman Coulter LX 20 (immunoturbidimetric), and Siemens Immulite (competitive immunoassay). Results were compared to values obtained by protein-cleavage liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS:In general, results from the 3 immunoassays agreed with results from LC-MS/MS. However, the SPQ results showed a negative bias across all ranges of albuminuria [(0-200 mg/L, y = 0.91x - 3.74 (CI 0.86-0.96); > 200 mg/L, y = 0.88x - 40.30 (CI 0.76-1.00)], whereas the LX 20 showed minimal bias in the 0-200 mg/L range [y = 0.97x - 88 (CI 0.92-1.02)] and the Immulite assay showed positive bias in the 0-200 mg/L range [y = 1.15x - 4.38 (CI 1.09-1.20)]. CONCLUSIONS:These results showed a reasonable quantification of urinary albumin by representative polyclonal and monoclonal immunoassays compared to an LC-MS/MS assay. In addition, the results do not suggest the presence of nonimmunoreactive albumin in urine. However, differences in analytic performance between assays support the need for a reference calibration material and reference method to standardize clinical laboratory measurements of urinary albumin.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Seegmiller JC,Sviridov D,Larson TS,Borland TM,Hortin GL,Lieske JC

doi

10.1373/clinchem.2009.129833

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

1991-4

issue

11

eissn

0009-9147

issn

1530-8561

pii

clinchem.2009.129833

journal_volume

55

pub_type

杂志文章
  • Distinguishing Non-Small Cell Lung Cancer Subtypes in Fine Needle Aspiration Biopsies by Desorption Electrospray Ionization Mass Spectrometry Imaging.

    abstract:BACKGROUND:Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small cell lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for lung cancer diagnosis and subtyping. Yet, histologic distinction of...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1093/clinchem/hvaa207

    authors: Bensussan AV,Lin J,Guo C,Katz R,Krishnamurthy S,Cressman E,Eberlin LS

    更新日期:2020-11-01 00:00:00

  • Hemoglobin Görwihl [alpha2beta(2)5(A2)Pro-->Ala], an electrophoretically silent variant with impaired glycation.

    abstract:BACKGROUND:Some of the genetic variants of hemoglobin (Hb) and their chemically modified species are known to affect the measurement of Hb A(1c). The purpose of this study was to characterize Hb species in the blood sample of a 74-year-old German male with an exceptionally low Hb A(1c) value. METHODS:Hemolysates from ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/49.1.137

    authors: Bissé E,Schauber C,Zorn N,Epting T,Eigel A,Van Dorsselaer A,Wieland H,Kister J,Kiger L

    更新日期:2003-01-01 00:00:00

  • Transferability studies for the AACC reference method and the IFCC method for measurement of alkaline phosphatase activity.

    abstract::We present the results of measurements of alkaline phosphatase activity from interlaboratory transferability studies conducted by 12 laboratories in five countries. The variability, as demonstrated by within-day precision (CV less than or equal to 2.6%), between-day precision (CV less than or equal to 3.6%), and betwe...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Tietz NW,Rinker AD,Burtis C,Duncan P,Ervin K,Ewen L,Hørder M,Mathieu M,Petitclerc C,Grisley D

    更新日期:1984-05-01 00:00:00

  • Immunochemical detection of group I and group II phospholipases A2 in human serum.

    abstract::Time-resolved fluoroimmunoassay was developed for the detection of synovial-type phospholipase A2 (s-PLA2) in human serum. This solid-phase, sandwich assay uses a polyclonal rabbit antibody raised against synovial-type group II PLA2 produced in Escherichia coli. No cross-reactions were detected between s-PLA2 and PLA2...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Nevalainen TJ,Kortesuo PT,Rintala E,Märki F

    更新日期:1992-09-01 00:00:00

  • Evidence-based laboratory medicine in oncology drug development: from biomarkers to diagnostics.

    abstract:BACKGROUND:The promise of targeted therapies in molecularly defined subsets of cancer has led to a transformation of the process of drug development in oncology. To target cancer successfully and precisely requires high-quality translational data. Such data can be generated by the use of biomarkers that answer key ques...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2012.191072

    authors: Modur V,Hailman E,Barrett JC

    更新日期:2013-01-01 00:00:00

  • Novel technique for scanning of codon 634 of the RET protooncogene with fluorescence resonance energy transfer and real-time PCR in patients with medullary thyroid carcinoma.

    abstract:BACKGROUND:The multiple endocrine neoplasia 2 (MEN 2) syndromes [MEN 2A, MEN 2B, and familial medullary thyroid carcinoma (FMTC)] are caused by germline mutations of the RET protooncogene. Because 85% of MEN 2A patients and 30% of FMTC patients have mutations at codon 634, the recommended molecular analyses begin at ex...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Ruiz A,Antiñolo G,Marcos I,Borrego S

    更新日期:2001-11-01 00:00:00

  • Dried plasma spot measurements of ferritin and transferrin receptor for assessing iron status.

    abstract:BACKGROUND:Efforts to reduce the high global prevalence of nutritional anemia require the use of both reliable laboratory assays to distinguish iron deficiency from other causes of anemia and cost-effective methods for collection of blood specimens under field conditions. The suitability of using small plasma samples s...

    journal_title:Clinical chemistry

    pub_type: 临床试验,杂志文章

    doi:

    authors: Flowers CH,Cook JD

    更新日期:1999-10-01 00:00:00

  • Nanofluidic digital PCR for KRAS mutation detection and quantification in gastrointestinal cancer.

    abstract:BACKGROUND:Concomitant quantification of multiple mutant KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) alleles may provide information in addition to that provided by standard mutation-detection procedures. We assessed the feasibility of a nanofluidic digital PCR array platform to detect and quantify KRAS...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2012.186577

    authors: Azuara D,Ginesta MM,Gausachs M,Rodriguez-Moranta F,Fabregat J,Busquets J,Pelaez N,Boadas J,Galter S,Moreno V,Costa J,de Oca J,Capellá G

    更新日期:2012-09-01 00:00:00

  • Microassay for nuclear binding of steroid receptors with use of intact cells from small samples of avian and human tissue.

    abstract::A micro version of a nuclear binding assay to assess the biological activity of receptors for steroid hormones was developed for application to small (needle) biopsies of human tumors for the purpose of predicting responses to steroid therapy. This easier assay requires 10-fold less tissue than the original nuclear bi...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Colvard DS,Jankus WR,Berg NJ,Graham ML 2nd,Jiang NS,Ingle JN,Spelsberg TC

    更新日期:1988-02-01 00:00:00

  • Four methods for glucose assay compared for various glucose concentrations and under different clinical conditions.

    abstract::Glucose was measured by the ferricyanide, the Beckman glucose oxidase, and the hexokinase procedures in 228 plasma samples taken during standard oral glucose-tolerance tests in 17 normal subjects and in 21 chemical diabetics. The neocuproine method was also used to measure glucose concentration in 156 samples (78 befo...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Giampietro O,Pilo A,Buzzigoli G,Boni C,Navalesi R

    更新日期:1982-12-01 00:00:00

  • Monoclonal antibody inhibiting creatine kinase MM3 but not isoform MM1.

    abstract::Monoclonal antibody CKM-G01 inhibited greater than 99% of the activity of porcine and human creatine kinase(CK)-MM isoenzyme purified from muscle. However, it inhibited only 54% of CK-MM in human serum. Chromatofocusing of serum CK-MM showed that CKM-G01 inhibited 100% of MM3 but not isoform MM1. CKM-G01 inhibited CK-...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Suzuki T,Shiraishi T,Tomita K,Totani M,Murachi T

    更新日期:1990-01-01 00:00:00

  • Detection of hemoglobin-based oxygen carriers in human serum for doping analysis: confirmation by size-exclusion HPLC.

    abstract:BACKGROUND:Hemoglobin-based oxygen carriers (HBOCs) are being developed as potential substitutes for the oxygen-carrying functions of erythrocytes, but athletes may obtain and experiment with HBOCs as an illicit means of enhancing oxygen transport. An electrophoretic technique has been developed to screen for the prese...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:10.1373/clinchem.2003.026591

    authors: Varlet-Marie E,Ashenden M,Lasne F,Sicart MT,Marion B,de Ceaurriz J,Audran M

    更新日期:2004-04-01 00:00:00

  • Polyol concentrations in serum during hemodialysis.

    abstract::Polyol concentrations were determined by selected-ion monitoring in 10 sample sets taken before dialysis and hourly for 4 h during dialysis. Predialysis concentrations (mumol/L) exceeding the upper limit of normal (mean + 2 SD; n = 33) were found for erythritol (25.4-59.0), threitol (11.5-46.7), and arabinitol (21.0-4...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Roboz J,Kappatos D,Holland JF

    更新日期:1990-12-01 00:00:00

  • [13C]Nandrolone excretion in trained athletes: interindividual variability in metabolism.

    abstract:BACKGROUND:Nandrolone is one of the most abused anabolic steroids, and its use in doping is increasing, as revealed by numerous positive cases during recent years in various sports. Different authors have reported the possible natural production of nandrolone metabolites in humans, and some of these authors argued that...

    journal_title:Clinical chemistry

    pub_type: 临床试验,杂志文章

    doi:10.1373/clinchem.2003.022848

    authors: Baume N,Avois L,Schweizer C,Cardis C,Dvorak J,Cauderay M,Mangin P,Saugy M

    更新日期:2004-02-01 00:00:00

  • Strand displacement amplification (SDA) and transient-state fluorescence polarization detection of Mycobacterium tuberculosis DNA.

    abstract::Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oligodeoxynucleotide detector probe hybridizes to the amplification prod...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Walker GT,Nadeau JG,Linn CP,Devlin RF,Dandliker WB

    更新日期:1996-01-01 00:00:00

  • For goodness' sake: expected therapeutic benefit as a basis for healthcare delivery.

    abstract::The current debate on our healthcare system has focused primarily on the cost of care. Because of drastically rising costs and their burden on our economy, government and the private sector have developed many approaches to reduce these costs. Managed care, special contracting arrangements, and government fiat have al...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:

    authors: Denton TA,Diamond GA

    更新日期:1995-05-01 00:00:00

  • Development and validation of an immunoradiometric assay of parathyrin-related protein in unextracted plasma.

    abstract::This two-site immunoradiometric assay for human parathyrin-related protein 1-86 (PTHRP1-86) in plasma uses a mouse monoclonal antibody to PTHRP1-34 coupled to cellulose particles for immunoextraction of N-terminal immunoreactivity, and a rabbit antiserum to PTHRP37-67 that is indirectly labeled with 125I-labeled PTHRP...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Ratcliffe WA,Norbury S,Heath DA,Ratcliffe JG

    更新日期:1991-05-01 00:00:00

  • Biochemical and genetic markers of iron status and the risk of coronary artery disease: an angiography-based study.

    abstract:BACKGROUND:Iron may promote coronary atherosclerotic disease (CAD) by increasing lipid peroxidation. Studies on biochemical or genetic markers of body iron stores as risk factors for CAD have yielded conflicting results. METHODS:We studied 849 individuals with a clear-cut definition of the CAD phenotype, i.e., with (C...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Bozzini C,Girelli D,Tinazzi E,Olivieri O,Stranieri C,Bassi A,Trabetti E,Faccini G,Pignatti PF,Corrocher R

    更新日期:2002-01-01 00:00:00

  • Liquid-chromatographic determination of free and total 3,4-dihydroxyphenylglycol and 3-methoxy-4-hydroxyphenylglycol in urine.

    abstract::We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Julien C,Rodriguez C,Sacquet J,Cuisinaud G,Sassard J

    更新日期:1988-05-01 00:00:00

  • Determination of quinidine by high-performance liquid chromatography.

    abstract::Quinidine in serum was measured at 330 nm, after protein precipitation, with an alkyl phenyl reversed-phase chromatographic column by peak-height determination (external standards.) Sensitivity was 0.3 mg/liter, with linear response to at least 10 mg/liter serum. Interassay precision, measured on 20 consecutive days, ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Powers JL,Sadee W

    更新日期:1978-02-01 00:00:00

  • Radial-immunodiffusion assay of human apolipoprotein A-I with use of two monoclonal antibodies combined.

    abstract::We produced and characterized several monoclonal antibodies directed toward human plasma apolipoprotein A-I. Two of them, A-I-12 and A-I-57, individually precipitated purified or native high-density lipoprotein in agarose gel by double immunodiffusion. Because radial immunodiffusion performed with a single monoclonal ...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Marcovina S,Di Cola G,Catapano AL

    更新日期:1986-12-01 00:00:00

  • Is diabetes mellitus a continuous spectrum?

    abstract:BACKGROUND:Diabetes mellitus has been historically divided into type 1 and type 2 diabetes, with type 1 being an autoimmune disease and type 2 being primarily a metabolic disease. CONTENT:The current diabetes classification scheme needs to be reevaluated because of the accumulating evidence of immune system involvemen...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2010.148270

    authors: Brooks-Worrell B,Palmer JP

    更新日期:2011-02-01 00:00:00

  • Use of natriuretic peptides to guide and monitor heart failure therapy.

    abstract:BACKGROUND:Plasma B-type cardiac natriuretic peptides reflect cardiac structure and function and have proven roles in assisting in the diagnosis of acute heart failure. They are independent prognostic indicators across the full spectrum of cardiovascular disease. Serial changes in plasma B-type cardiac natriuretic pept...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2011.165704

    authors: Richards AM,Troughton RW

    更新日期:2012-01-01 00:00:00

  • Semiautomated spectrophotometry of total phospholipids in plasma.

    abstract::A one-stage semiautomated method for estimating total phospholipids in plasma is reported. The phospholipids are extracted into aqueous ethanol, combined with a Lewis acid, and the change in pH is monitored colorimetrically. The method, compared with the acid digestion method of Gomori [J. Lab. Clin. Med. 27, 955 (194...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Wootton DG

    更新日期:1977-09-01 00:00:00

  • A step forward in enzymatic measurement of creatinine.

    abstract::We describe an improved enzymatic ultraviolet absorbance method for assaying creatinine in serum, plasma, and urine. Creatinine is hydrolyzed by creatinine iminohydrolase (EC 3.5.4.21) to ammonia and N-methylhydantoin. The ammonia produced combines with 2-oxoglutarate and NADPH in the presence of glutamate dehydrogena...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Fossati P,Ponti M,Passoni G,Tarenghi G,Melzi d'Eril GV,Prencipe L

    更新日期:1994-01-01 00:00:00

  • Magnetizable solid-phase fluoroimmunoassay of thyroxine by a sequential addition technique.

    abstract::We describe a simple fluoroimmunoassay for the determination of thyroxine concentrations in serum. The method, "sequential addition, separation fluoroimmunoassay," involves both thyroxine labeled with fluorescein and magnetizable cellulose/iron oxide particles to which antibodies to thyroxine have been covalently link...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Nargessi RD,Ackland J,Hassan M,Forrest GC,Smith DS,Landon J

    更新日期:1980-11-01 00:00:00

  • Advances in the Treatment of Hemophilia: Implications for Laboratory Testing.

    abstract:BACKGROUND:Until recently, clinical laboratories have monitored hemophilia treatment by measuring coagulation factors before/after infusion of human-derived or recombinant factors. Substantial changes are expected in the near future based on new therapeutic approaches that have been or are being developed. CONTENT:Hem...

    journal_title:Clinical chemistry

    pub_type: 杂志文章,评审

    doi:10.1373/clinchem.2017.284356

    authors: Tripodi A,Chantarangkul V,Novembrino C,Peyvandi F

    更新日期:2019-02-01 00:00:00

  • Use of the caffeine reagent in direct spectrophotometry of bilirubin.

    abstract::The molar absorptivity of unconjugated bilirubin in caffeine reagent is independent of the protein matrix. This finding, together with the simplicity of a dilution step for direct spectrophotometry, will improve the calibration methods of bilirubin and make them more nearly accurate. This is encouraging for the develo...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Vink KL,Schuurman W,van Gansewinkel R

    更新日期:1986-07-01 00:00:00

  • Thyroxine, thyrotropin, and age in a euthyroid hospital patient population.

    abstract::The diagnosis of thyroid disease now often can be achieved reliably by measuring thyrotropin (TSH) alone. Thyroxine (T4), triiodothyronine, and other analytes are only needed if TSH and the accompanying clinical condition are discordant. We describe here work that confirms the age independence of TSH in both inpatient...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Davey R

    更新日期:1997-11-01 00:00:00

  • Molar absorptivity and the blank correction factor.

    abstract::In photometry, where both the product formed and one or several reactants absorb light at the same wavelength, the absorbance of the "blank" of the sample at the end of the reaction may be less than that measured at the beginning of the reaction, because of consumption of reactant(s). The blank correction factor for t...

    journal_title:Clinical chemistry

    pub_type: 杂志文章

    doi:

    authors: Kroll MH,Elin RJ

    更新日期:1985-03-01 00:00:00