Abstract:
BACKGROUND:Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. METHODS:We quantified urinary albumin in patient samples by using 3 commercially available reagent systems: DiaSorin SPQ and Beckman Coulter LX 20 (immunoturbidimetric), and Siemens Immulite (competitive immunoassay). Results were compared to values obtained by protein-cleavage liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS:In general, results from the 3 immunoassays agreed with results from LC-MS/MS. However, the SPQ results showed a negative bias across all ranges of albuminuria [(0-200 mg/L, y = 0.91x - 3.74 (CI 0.86-0.96); > 200 mg/L, y = 0.88x - 40.30 (CI 0.76-1.00)], whereas the LX 20 showed minimal bias in the 0-200 mg/L range [y = 0.97x - 88 (CI 0.92-1.02)] and the Immulite assay showed positive bias in the 0-200 mg/L range [y = 1.15x - 4.38 (CI 1.09-1.20)]. CONCLUSIONS:These results showed a reasonable quantification of urinary albumin by representative polyclonal and monoclonal immunoassays compared to an LC-MS/MS assay. In addition, the results do not suggest the presence of nonimmunoreactive albumin in urine. However, differences in analytic performance between assays support the need for a reference calibration material and reference method to standardize clinical laboratory measurements of urinary albumin.
journal_name
Clin Chemjournal_title
Clinical chemistryauthors
Seegmiller JC,Sviridov D,Larson TS,Borland TM,Hortin GL,Lieske JCdoi
10.1373/clinchem.2009.129833subject
Has Abstractpub_date
2009-11-01 00:00:00pages
1991-4issue
11eissn
0009-9147issn
1530-8561pii
clinchem.2009.129833journal_volume
55pub_type
杂志文章abstract:BACKGROUND:Distinguishing adenocarcinoma and squamous cell carcinoma subtypes of non-small cell lung cancers is critical to patient care. Preoperative minimally-invasive biopsy techniques, such as fine needle aspiration (FNA), are increasingly used for lung cancer diagnosis and subtyping. Yet, histologic distinction of...
journal_title:Clinical chemistry
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doi:10.1373/clinchem.2012.186577
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journal_title:Clinical chemistry
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1982-12-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1990-01-01 00:00:00
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更新日期:2004-04-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1990-12-01 00:00:00
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pub_type: 临床试验,杂志文章
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journal_title:Clinical chemistry
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doi:
更新日期:1996-01-01 00:00:00
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doi:
更新日期:1995-05-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1991-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:2002-01-01 00:00:00
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journal_title:Clinical chemistry
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1978-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-12-01 00:00:00
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doi:
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journal_title:Clinical chemistry
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doi:
更新日期:1994-01-01 00:00:00
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doi:
更新日期:1980-11-01 00:00:00
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更新日期:2019-02-01 00:00:00
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journal_title:Clinical chemistry
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doi:
更新日期:1986-07-01 00:00:00
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journal_title:Clinical chemistry
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更新日期:1997-11-01 00:00:00
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doi:
更新日期:1985-03-01 00:00:00