Determination of 5-hydroxytryptophan in plasma by high-performance liquid chromatography and fluorometric detection after phthaldialdehyde reaction.

Abstract:

:We describe the sensitive and precise determination 5-hydroxytryptophan in plasma from patients treated with this compound. The method is based on solvent extraction of plasma, high-performance liquid chromatography and continuous fluorometric detection after reaction with phthaldialdehyde in strong acid. The combined time for chromatography and reaction is 25 min. The sensitivity of the method is adequate to quantitate 0.05 mumol of 5-hydroxytryptophan per liter, in 750 microliter of plasma. No interference by other hydroxyindole compounds was found.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Enbaek F,Magnussen I

subject

Has Abstract

pub_date

1978-02-01 00:00:00

pages

376-8

issue

2

eissn

0009-9147

issn

1530-8561

journal_volume

24

pub_type

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