Medicare's demonstration of competitive bidding for clinical laboratory services: what it means for clinical laboratories.

abstract：BACKGROUND:The short half-life of the alpha-class glutathione S-transferases (GSTAs) in plasma combined with their even distribution throughout the liver lobule suggests that they may be useful complements to the more traditionally used liver markers. However, the currently available assays for measuring GSTAs in biolo...

journal_title：Clinical chemistry

authors： Dajani LK,Paus E,Warren DJ

更新日期：2001-05-01 00:00:00

abstract：BACKGROUND:We evaluated the completeness of reporting of diagnostic test accuracy (DTA) systematic reviews using the recently developed Preferred Reporting Items for Systematic Reviews and MetaAnalyses (PRISMA)-DTA guidelines. METHODS:MEDLINE® was searched for DTA systematic reviews published October 2017 to January 2...

journal_title：Clinical chemistry

authors： Salameh JP,McInnes MDF,Moher D,Thombs BD,McGrath TA,Frank R,Dehmoobad Sharifabadi A,Kraaijpoel N,Levis B,Bossuyt PM

更新日期：2019-02-01 00:00:00

abstract：:We measured total serum bile acids on a fluorescence-light-scattering micro centrifugal analyzer by the direct enzymatic method with 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) and with resazurin as a fluorogenic electron acceptor. We found that serum protein has an inhibitory effect on the measurement of bile ...

journal_title：Clinical chemistry

authors： Hanson NQ,Freier EF

更新日期：1983-01-01 00:00:00

abstract：:We present a rate-determination method for analyzing glucose. A glucose enzyme electrode serves as the sensor and is made by placing a gel-immobilized layer of glucose oxidase over the tip of a Clark-type O2 electrode. The electrode membrane is made of Teflon and is derivatized by etching with a suspension of colloida...

journal_title：Clinical chemistry

authors： Sokol L,Garber C,Shults M,Updike S

更新日期：1980-01-01 00:00:00

abstract：:We review the following labels used as substitutes for radioisotopes in immunoassay systems: bacteriophages, chemiluminescence precursors, fluorochromes, fluorogens, fluorescence quenchers, enzymes, coenzymes, inhibitors, substrates, various particulates, metal atoms, and stable free radicals. New methods for performi...

journal_title：Clinical chemistry

authors： Schall RF Jr,Tenoso HJ

更新日期：1981-07-01 00:00:00

abstract：BACKGROUND:Increased urinary albumin excretion is a well-documented diagnostic and prognostic biomarker for renal disease. Urinary albumin is typically measured in clinical settings by immunoassay methods. However, neither a reference method nor a urine albumin calibration reference material is currently available. ME...

journal_title：Clinical chemistry

authors： Seegmiller JC,Sviridov D,Larson TS,Borland TM,Hortin GL,Lieske JC

更新日期：2009-11-01 00:00:00

abstract：:In the present clinical evaluation of Triage immunoassay for detection of drugs of abuse, we examined a possible connection between the laboratory skills of the personnel using it and the quality of analytical results; we also evaluated the degree of concordance between the Triage results and those obtained by an inst...

journal_title：Clinical chemistry

authors： de la Torre R,Domingo-Salvany A,Badia R,Gonzàlez G,McFarlane D,San L,Torrens M

更新日期：1996-09-01 00:00:00

abstract：BACKGROUND:The accurate and precise measurement of urinary albumin is critical, since even minor increases are diagnostically sensitive indicators of renal disease, cardiovascular events, and risk for death. To gain insights into potential measurement biases, we systematically compared urine albumin measurements perfor...

journal_title：Clinical chemistry

authors： Shaikh A,Seegmiller JC,Borland TM,Burns BE,Ladwig PM,Singh RJ,Kumar R,Larson TS,Lieske JC

更新日期：2008-09-01 00:00:00

abstract：:We describe a simple fluoroimmunoassay for the determination of thyroxine concentrations in serum. The method, "sequential addition, separation fluoroimmunoassay," involves both thyroxine labeled with fluorescein and magnetizable cellulose/iron oxide particles to which antibodies to thyroxine have been covalently link...

journal_title：Clinical chemistry

authors： Nargessi RD,Ackland J,Hassan M,Forrest GC,Smith DS,Landon J

更新日期：1980-11-01 00:00:00

abstract：:3-Hydroxy-3-methylglutaric aciduria was detected in a newborn. The progress of the dietary therapy for the disorder was monitored by dual-column "high-performance" liquid chromatography with a computer-controlled photodiode array spectrophotometric detector. This procedure is a quick way to detect and monitor the prog...

journal_title：Clinical chemistry

authors： Buchanan DN,Thoene JG

更新日期：1986-01-01 00:00:00

abstract：BACKGROUND:Although numerous lateral flow immunoassays (LFIAs) have been developed and widely used, inadequate analytical sensitivity and the lack of multiple protein detection applications have limited their clinical utility. We developed a new LFIA device for the simultaneous detection of high-sensitivity cardiac tro...

journal_title：Clinical chemistry

authors： Zhu J,Zou N,Zhu D,Wang J,Jin Q,Zhao J,Mao H

更新日期：2011-12-01 00:00:00

abstract：:We have developed a succinyldicholine-based assay for serum cholinesterase (EC 3.1.1.8) to help establish whether patients with suspected sensitivity to drugs of this type have enzyme abnormalities that cannot be detected by conventional laboratory techniques. Although the method discriminates between cholinesterase a...

journal_title：Clinical chemistry

authors： Faye S,Evans RT

更新日期：1986-08-01 00:00:00

abstract：:Seeking to minimize degradation of NADPH during storage, reagent preparation, and assays, we investigated the effects of pH, temperature, and ionic strength as well as the effects of phosphate and acetate. NADH was also included for comparison. Our results indicate that the rate of degradation of NADPH is proportional...

journal_title：Clinical chemistry

authors： Wu JT,Wu LH,Knight JA

更新日期：1986-02-01 00:00:00

abstract：:To see if saliva is a valid substitute for plasma in assay of progesterone even when concentrations of hormone and binding proteins are fluctuating, we determined the concentrations of total and free progesterone in plasma and salivary progesterone in specimens from 36 women volunteers during the course of pregnancy a...

journal_title：Clinical chemistry

authors： Meulenberg PM,Hofman JA

更新日期：1989-01-01 00:00:00

abstract：BACKGROUND:High circulating concentrations of N-terminal fragments of A- and B-type natriuretic peptides (NT-proANP and NT-proBNP) identify patients with impaired cardiac function. ProANP-derived peptides are particularly sensitive to increased preload of the heart and proBNP-derived peptides to increased afterload; th...

journal_title：Clinical chemistry

authors： Ala-Kopsala M,Ruskoaho H,Leppäluoto J,Seres L,Skoumal R,Toth M,Horkay F,Vuolteenaho O

更新日期：2005-04-01 00:00:00

abstract：:We describe the conjoint measurement in urine of the alcoholic catecholamine metabolites 3,4-dihydroxyphenylglycol (DHPG) and 3-methoxy-4-hydroxyphenylglycol (MHPG). The method involves extraction with ethyl acetate and back-extraction into acetic acid (MHPG), followed by adsorption onto alumina (DHPG). The separation...

journal_title：Clinical chemistry

authors： Julien C,Rodriguez C,Sacquet J,Cuisinaud G,Sassard J

更新日期：1988-05-01 00:00:00

abstract：BACKGROUND:The alteration of the bone microenvironment as a consequence of skeletal metastases is poorly understood. The aim of this study was to search for patterns of bone markers in relation to primary tumor type, bone pain, and number of sites involved in patients with bone metastases. METHODS:We studied 323 patie...

journal_title：Clinical chemistry

authors： Berruti A,Dogliotti L,Gorzegno G,Torta M,Tampellini M,Tucci M,Cerutti S,Frezet MM,Stivanello M,Sacchetto G,Angeli A

更新日期：1999-08-01 00:00:00

abstract：BACKGROUND:It is important that cardiac troponin be measured accurately with a robust method to limit false results with potentially adverse clinical outcomes. In this study, we characterized the robustness of 4 analytical platforms by measuring the outlier rate between duplicate results. METHODS:We measured cardiac t...

journal_title：Clinical chemistry

authors： Pretorius CJ,Dimeski G,O'Rourke PK,Marquart L,Tyack SA,Wilgen U,Ungerer JP

更新日期：2011-05-01 00:00:00

abstract：:Creatinine and specific gravity (relative density) measurements both allow differences in urine concentration to be taken into account in determining urine cotinine concentrations. In this study we demonstrate that the variance of urine cotinine measurements is reduced comparably when either creatinine or specific gra...

journal_title：Clinical chemistry

authors： Haddow JE,Knight GJ,Palomaki GE,Neveux LM,Chilmonczyk BA

更新日期：1994-04-01 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) was measured in plasma by a new immunoradiometric assay (IRMA) from Nichols Institute. The assay is specific for PTHrP and shows excellent parallelism when measuring keratinocyte fluid, samples with high PTHrP content, and PTHrP-supplemented plasma. A precision profile estab...

journal_title：Clinical chemistry

authors： Fraser WD,Robinson J,Lawton R,Durham B,Gallacher SJ,Boyle IT,Beastall GH,Logue FC

更新日期：1993-03-01 00:00:00

abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...

journal_title：Clinical chemistry

authors： Ritter D,Lee PA,Taylor JF,Hsu L,Cohen JD,Chung HD,Virgo KS

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Heparinized plasma samples allow more rapid analysis than serum samples, but preliminary studies showed lower cardiac troponin T (cTnT) results in plasma. We undertook a multicenter study to characterize this effect for cTnT and cardiac troponin I (cTnI). METHODS:Blood samples were collected with and withou...

journal_title：Clinical chemistry

authors： Gerhardt W,Nordin G,Herbert AK,Burzell BL,Isaksson A,Gustavsson E,Haglund S,Müller-Bardorff M,Katus HA

更新日期：2000-06-01 00:00:00

abstract：BACKGROUND:Eph receptors and their ligands, the ephrins, represent a large class of cell-cell communication molecules with well-defined developmental functions. Their role in healthy adult tissues and in human disease is still largely unknown, although diverse roles in carcinogenesis have been postulated. METHODS:We e...

journal_title：Clinical chemistry

authors： Hafner C,Schmitz G,Meyer S,Bataille F,Hau P,Langmann T,Dietmaier W,Landthaler M,Vogt T

更新日期：2004-03-01 00:00:00

abstract：:We describe a case of sulfhemoglobinemia associated with toxic paint ingestion. Blood gases, oxygen content, and fractional hemoglobin derivatives were assayed with Radiometer 520 and OSM3 instruments. Although the CO-oximeters indicated the presence of sulfhemoglobin (SulfHb), the results were not quantitative. An OS...

journal_title：Clinical chemistry

authors： Wu C,Kenny MA

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:It has been postulated that changes in S-adenosylhomocysteine (AdoHcy), a potent inhibitor of transmethylation, provide a mechanism by which increased homocysteine causes its detrimental effects. We aimed to develop a rapid and sensitive method to measure AdoHcy and its precursor S-adenosylmethionine (AdoMet...

journal_title：Clinical chemistry

authors： Gellekink H,van Oppenraaij-Emmerzaal D,van Rooij A,Struys EA,den Heijer M,Blom HJ

更新日期：2005-08-01 00:00:00

abstract：BACKGROUND:Atherosclerosis is a systemic disease with focal rupture of vulnerable plaque responsible for major clinical events. Several population-based studies indicate an association between lipoprotein-associated phospholipase A2 (Lp-PLA2) and cardiovascular events. Lp-PLA2 is emerging as a biomarker that may be a p...

journal_title：Clinical chemistry

authors： Zalewski A,Nelson JJ,Hegg L,Macphee C

更新日期：2006-09-01 00:00:00

abstract：:We have evaluated a new immunoenzymatic assay for human thyrotropin involving three monoclonal antibodies (Abbott HTSH EIA) and compared the results with those of two conventional nonequilibrium double-antibody radioimmunoassay (RIA) methods: Clinical Assays' RIA and a research RIA (J Clin Endocrinol Metab 1975; 41:67...

journal_title：Clinical chemistry

authors： Pekary AE,Turner LF Jr,Hershman JM

更新日期：1986-03-01 00:00:00

abstract：:Radioimmunoassay of triiodothyronine was performed with a homogeneous incubation system. Bound and free ligand were separated by partitioning in an aqueous two-phase system. The agreement between the results obtained with this technique and conventional heterogeneous radioimmunoassay was very good (r = 0.981). ...

journal_title：Clinical chemistry

authors： Mattiasson B,Eriksson H

更新日期：1982-04-01 00:00:00

abstract：BACKGROUND:Since its beginnings, newborn screening for cystic fibrosis (CF) using an assay for immunoreactive trypsinogen (IRT) has been plagued by a high rate of false-positive results (screen positive, diagnosis negative), despite attempts to reduce this rate by use of altered cutoffs and second-tier DNA testing. IRT...

journal_title：Clinical chemistry

authors： Lindau-Shepard BA,Pass KA

更新日期：2010-03-01 00:00:00