Photodiode array detection for liquid chromatographic profiling of carboxylic acids in physiological fluids: 3-hydroxy-3-methylglutaric aciduria.

abstract：:We present evidence for the utility of an improved assay for the activity of lactate dehydrogenase (EC 1.1.1.27) isoenzymes 1 and 2 in serum, involving inhibition of the H-subunit of LD by pyruvate at pH 7.1. Results correlate well with the LD-1/total LD ratio as evaluated by immunological assay and, as an index to in...

journal_title：Clinical chemistry

authors： Bernstein LH,Scinto P

更新日期：1986-05-01 00:00:00

abstract：:We compared a chemiluminescent assay and a colorimetric endpoint assay for measuring an alkaline phosphatase (EC 3.1.3.1) label in an enzyme immunoassay of thyrotropin (TSH). The substrate in the chemiluminescent assay is a derivative of adamantyl 1,2-dioxetane phosphate. On dephosphorylation, catalyzed by alkaline ph...

journal_title：Clinical chemistry

authors： Bronstein I,Voyta JC,Thorpe GH,Kricka LJ,Armstrong G

更新日期：1989-07-01 00:00:00

abstract：:A continuous-monitoring method for measuring acid phosphatase activity with alpha-naphthyl phosphate as the substrate was critically evaluated and modified. Using partially purified prostatic acid phosphatase, we show that certain conditions for the assay must be satisfied to ensure linearity. These conditions include...

journal_title：Clinical chemistry

authors： Bais R,Edwards JB

更新日期：1976-12-01 00:00:00

abstract：:As the clinical utility of glycated hemoglobin (gHb) measurement increases, so does the need for standardization of values between different methods and different laboratories. Using three different methods, we examined the feasibility of interlaboratory standardization of gHb measurement. A liquid-chromatographic (HP...

journal_title：Clinical chemistry

authors： Little RR,England JD,Wiedmeyer HM,McKenzie EM,Mitra R,Erhart PM,Durham JB,Goldstein DE

更新日期：1986-02-01 00:00:00

abstract：:To demonstrate oligoclonal IgG bands (I) in unconcentrated cerebrospinal fluid, we used isoelectric focusing in agarose followed by protein transfer to cellulose nitrate membrane, double-antibody peroxidase labeling, and avidin-biotin amplification. I can be reliably seen after isoelectric focusing of 5-microL specime...

journal_title：Clinical chemistry

authors： Olsson T,Kostulas V,Link H

更新日期：1984-07-01 00:00:00

abstract：BACKGROUND:Clinical diagnoses were correlated with results of a Co(II)-albumin binding assay in 167 patients treated at an emergency department of a health maintenance organization. METHODS:Patients were evaluated as being nonischemic or potentially ischemic through standard coronary disease indicators [creatine kinas...

journal_title：Clinical chemistry

authors： Bhagavan NV,Lai EM,Rios PA,Yang J,Ortega-Lopez AM,Shinoda H,Honda SA,Rios CN,Sugiyama CE,Ha CE

更新日期：2003-04-01 00:00:00

abstract：:We describe an enzyme-linked two-site immunoassay for quantitation of intact insulin in human serum and plasma. The method uses two murine monoclonal antibodies that bind to two different epitopes on the insulin molecule. The immunoassay is specific. Human proinsulin is not bound by the antibodies, and the binding of ...

journal_title：Clinical chemistry

authors： Andersen L,Dinesen B,Jørgensen PN,Poulsen F,Røder ME

更新日期：1993-04-01 00:00:00

abstract：:We tested the diagnostic sensitivity of various urinary analytes for detecting cadmium-induced nephropathy at an early stage. We investigated 73 healthy persons (control group 1) and individuals exposed to cadmium, either environmentally (n = 36, risk group 2) or occupationally (n = 62, exposed group 3). All data were...

journal_title：Clinical chemistry

authors： Jung K,Pergande M,Graubaum HJ,Fels LM,Endl U,Stolte H

更新日期：1993-05-01 00:00:00

abstract：BACKGROUND:Recent studies have suggested that single-stranded DNA (ssDNA) library preparation can enrich short DNA species from the plasma of healthy individuals, cancer patients, and transplant recipients. Based on previous observations that fetal DNA molecules in the maternal plasma are shorter than maternal DNA mole...

journal_title：Clinical chemistry

authors： Vong JSL,Tsang JCH,Jiang P,Lee WS,Leung TY,Chan KCA,Chiu RWK,Lo YMD

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:Concerns exist regarding how the 99th percentile upper reference limit (URL) of cardiac troponin (cTn) is determined and whether it should be derived from normal healthy individuals. CONTENT:The 99th percentile URL of cTn is an important criterion to standardize the diagnosis of myocardial infarction (MI) f...

journal_title：Clinical chemistry

authors： Sandoval Y,Apple FS,Saenger AK,Collinson PO,Wu AHB,Jaffe AS

更新日期：2020-09-01 00:00:00

abstract：:We report the presence of complexes between aspartate aminotransferase (AST, EC 2.6.1.1) and immunoglobulin (Ig) in the serum of a patient suffering from lung cancer with metastasis to the liver. After fractionation of the serum by gel filtration, AST-Ig complexes (AST-IgA, AST-IgG) were demonstrated by counterimmunoe...

journal_title：Clinical chemistry

authors： Nagamine M,Okochi K

更新日期：1983-02-01 00:00:00

abstract：:Hepatitis A virus (HAV) infection occurs worldwide and is an important cause of acute viral hepatitis in the US. In this review, I cover the epidemiology, course of infection, clinical manifestations, serological responses, and prevention of this infection. Although most patients completely recover from this disease, ...

journal_title：Clinical chemistry

authors： Lemon SM

更新日期：1997-08-01 00:00:00

abstract：BACKGROUND:In this study we demonstrate the technical application of flow cytometry and cell sorting combined with gene-rearrangement clonality profiling to detect and confirm minimal disease in 2 leukemia and 2 lymphoma cases. METHODS:Specimens with low percentages (0.05%-5%) of abnormal lymphoid populations were ide...

journal_title：Clinical chemistry

authors： Zehentner BK,Fritschle W,Stelzer T,Ghirardelli KM,Hunter K,Wentzel C,Bennington R,Hansen CL,Myerson D,Kalnoski M,Wells DA,Loken MR

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:Until recently, clinical laboratories have monitored hemophilia treatment by measuring coagulation factors before/after infusion of human-derived or recombinant factors. Substantial changes are expected in the near future based on new therapeutic approaches that have been or are being developed. CONTENT:Hem...

journal_title：Clinical chemistry

authors： Tripodi A,Chantarangkul V,Novembrino C,Peyvandi F

更新日期：2019-02-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Increasing numbers of patients are presenting worldwide to emergency departments with suspected myocardial infarction. The use of point-of-care troponin assays might enable faster decision-making in this high-risk population and reduce the burden on emergency facilities. Here, we evaluate the diagnostic perf...

journal_title：Clinical chemistry

authors： Sörensen NA,Neumann JT,Ojeda F,Giannitsis E,Spanuth E,Blankenberg S,Westermann D,Zeller T

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:The recent discovery of the presence of circulating cell-free fetal DNA in maternal plasma opens up new prenatal diagnostic applications and provides new avenues for clinical investigation. It is of research and potential diagnostic interest to determine whether fetal trisomy 21 may be associated with quanti...

journal_title：Clinical chemistry

authors： Lo YM,Lau TK,Zhang J,Leung TN,Chang AM,Hjelm NM,Elmes RS,Bianchi DW

更新日期：1999-10-01 00:00:00

abstract：BACKGROUND:Because reference values for vitamin B(12) concentrations and vitamin B(12)-binding capacities for pregnant women have not been established, the reference values for nonpregnant women are often applied to assess vitamin B(12) status. The aim of the present study was to describe ranges of biochemical indices ...

journal_title：Clinical chemistry

authors： Koebnick C,Heins UA,Dagnelie PC,Wickramasinghe SN,Ratnayaka ID,Hothorn T,Pfahlberg AB,Hoffmann I,Lindemans J,Leitzmann C

更新日期：2002-06-01 00:00:00

abstract：:Even though significant progress has been made in the development of electrodes for the in vivo detection of catecholamines, oxygen, and various cations, there has been little research on the feasibility of continuously monitoring drugs in whole blood by electrochemical sensing devices. Electroanalytical problems asso...

journal_title：Clinical chemistry

authors： Pinkerton TC,Lawson BL

更新日期：1982-09-01 00:00:00

abstract：:The tricyclic antidepressants amitriptyline, nortriptyline, imipramine, and desipramine in serum of patients taking one of the drugs were quantified in two laboratories by high-performance liquid chromatography (HPLC) and enzyme-multiplied immunoassay (EMIT; Syva). Results for split samples were highly correlated, but...

journal_title：Clinical chemistry

authors： Dorey RC,Preskorn SH,Widener PK

更新日期：1988-11-01 00:00:00

abstract：BACKGROUND:Lectin-like oxidized LDL receptor 1 (LOX-1) is implicated in atherothrombotic diseases. Activation of LOX-1 in humans can be evaluated by use of the LOX index, obtained by multiplying the circulating concentration of LOX-1 ligands containing apolipoprotein B (LAB) times that of the soluble form of LOX-1 (sLO...

journal_title：Clinical chemistry

authors： Inoue N,Okamura T,Kokubo Y,Fujita Y,Sato Y,Nakanishi M,Yanagida K,Kakino A,Iwamoto S,Watanabe M,Ogura S,Otsui K,Matsuda H,Uchida K,Yoshimoto R,Sawamura T

更新日期：2010-04-01 00:00:00

abstract：:A potential genetic marker for hypertriglyceridemia and coronary disease, an apolipoprotein-CIII gene allele displaying a single nucleotide substitution, was found in 71% of Arabs with primary hypertriglyceridemia. Over 96% of the unrelated adults possessing this allele showed hypertriglyceridemia. The same allele was...

journal_title：Clinical chemistry

authors： Taş S

更新日期：1989-02-01 00:00:00

abstract：BACKGROUND:Near-infrared spectroscopy is proposed as a method for providing real-time urea concentrations during hemodialysis treatments. The feasibility of such noninvasive urea measurements is evaluated in undiluted dialysate fluid. METHODS:Near-infrared spectra were collected from calibration solutions of urea prep...

journal_title：Clinical chemistry

authors： Eddy CV,Arnold MA

更新日期：2001-01-01 00:00:00

abstract：:We studied several risk factors in relation to parental cardiovascular heart disease: total cholesterol, triglycerides, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, apolipoprotein (apo) A-I, apo B, and lipoprotein(a) [Lp(a)] were determined in 322 serum samples (43 from subjec...

journal_title：Clinical chemistry

authors： Vella JC,Jover E

更新日期：1993-03-01 00:00:00

abstract：BACKGROUND:Extracellular vesicles (EVs) contain a rich cargo of different RNA species with specialized functions and clinical applications. However, the landscape and characteristics of extracellular vesicle long RNA (exLR) in human blood remain largely unknown. METHODS:We presented an optimized strategy for exLR sequ...

journal_title：Clinical chemistry

authors： Li Y,Zhao J,Yu S,Wang Z,He X,Su Y,Guo T,Sheng H,Chen J,Zheng Q,Li Y,Guo W,Cai X,Shi G,Wu J,Wang L,Wang P,He X,Huang S

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:The multiple endocrine neoplasia 2 (MEN 2) syndromes [MEN 2A, MEN 2B, and familial medullary thyroid carcinoma (FMTC)] are caused by germline mutations of the RET protooncogene. Because 85% of MEN 2A patients and 30% of FMTC patients have mutations at codon 634, the recommended molecular analyses begin at ex...

journal_title：Clinical chemistry

authors： Ruiz A,Antiñolo G,Marcos I,Borrego S

更新日期：2001-11-01 00:00:00

abstract：BACKGROUND:Vascular age is an emerging health indicator and predictor of end-organ damage to the heart, brain, and kidney. Although there have been many review publications concerning risk factors for vascular aging, most include cross-sectional epidemiological studies, limiting inferences about temporality. There is a...

journal_title：Clinical chemistry

authors： Kucharska-Newton AM,Stoner L,Meyer ML

更新日期：2019-01-01 00:00:00

abstract：:A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after ...

journal_title：Clinical chemistry

authors： Egberts J,Soederhuizen W,Gebhardt DO

更新日期：1983-02-01 00:00:00

abstract：BACKGROUND:C-peptide is a marker of insulin secretion in diabetic patients. We assessed within- and between-laboratory imprecision of C-peptide assays and determined whether serum calibrators with values assigned by mass spectrometry could be used to harmonize C-peptide results. METHODS:We sent 40 different serum samp...

journal_title：Clinical chemistry

authors： Little RR,Rohlfing CL,Tennill AL,Madsen RW,Polonsky KS,Myers GL,Greenbaum CJ,Palmer JP,Rogatsky E,Stein DT

更新日期：2008-06-01 00:00:00

abstract：:Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin ...

journal_title：Clinical chemistry

authors： Jortani SA,Helm RA,Valdes R Jr

更新日期：1996-10-01 00:00:00